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Porous nano-scale soft colloid and method for transporting cells or tissue culture by using porous nano-scale soft colloid

A technology of tissue culture and soft colloid, which is applied in the field of biomedical materials, can solve the problems that the physical environment cannot be well protected, and there is no cell transportation, so as to meet the requirements of long-distance transportation, reduction of fixation and physical protection, and maintenance of functionality. Effect

Pending Publication Date: 2020-06-16
DALIAN PRACTICAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the field of stem cell and tissue engineering treatment is an emerging treatment method within 5 years, there is no standard solution for specialized cell transportation. At present, there are only several corresponding cell transportation boxes to ensure and monitor the temperature changes during transportation, such as patent CN205150689U With the disclosed stem cell transport case such as CN206337257U
However, the physical environment of stem cells or tissue cells is often not well protected during transportation using such a transport box, which mainly means that stem cells or tissue cells are often mutually supported human tissues for protection. All of the transportation still uses liquid as the carrier for stem cells and tissue cells

Method used

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  • Porous nano-scale soft colloid and method for transporting cells or tissue culture by using porous nano-scale soft colloid
  • Porous nano-scale soft colloid and method for transporting cells or tissue culture by using porous nano-scale soft colloid
  • Porous nano-scale soft colloid and method for transporting cells or tissue culture by using porous nano-scale soft colloid

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Experimental program
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Effect test

Embodiment 1

[0034] Preparation of Porous Nanoscale Soft Colloids

[0035] The porous nano-scale soft colloid is synthesized by free radical emulsion polymerization multipolymer method, and the specific preparation process is: 9.9 millimoles of isopropylacrylamide NIPAM, 0.1 millimoles of negatively charged molecules (such as figure 1 shown), 0.2 mmol of 2-mercaptobenzoic acid MBA and 0.2 mmol of sodium dodecyl sulfate SDS were dissolved in 97 ml of deionized water. After thorough mixing, the mixed solution was transferred to a 250ml three-necked flask equipped with a condenser and a mechanical stirrer.

[0036] Before polymerization, the mixed solution was placed in a preheated 70°C oil bath. The mixed solution was then degassed under nitrogen for 30 minutes. After completion of the degassing, 3.0 ml of an aqueous solution containing 0.1 mmol of potassium persulfate KPS was injected into the mixture solution to initiate polymerization. The entire polymerization reaction was carried out...

Embodiment 2

[0037] The microstructure of embodiment 2 porous nanoscale soft colloids (acrylic acid grafted molecules)

[0038]The porous nano-scale honeycomb structure (acrylic acid grafted molecules) was observed with a scanning electron microscope (SEM) for multilayer cross-sections in the vertical and horizontal directions. Such as Figure 4 As shown, the porous nano-scale honeycomb structure has a nano-scale pore size and can support or accommodate cells or tissues. The porous nano-scale soft colloid has certain mechanical properties and can provide physical support to cells or tissues while reducing damage to cells or tissues caused by collisions and shear forces during transportation.

Embodiment 3

[0039] Example 3 The Process of Porous Nanoscale Colloid (Acrylic Acid Grafting Molecule) Carrying out Phase Change with Temperature

[0040] Use general stress rheometer to carry out dynamic oscillation experiment, temperature is controlled by Peltier system, detect elastic (storage) modulus G' and viscous (loss) modulus G" at different temperatures. Such as Figure 5 As shown, it is found that the embodiments of the present invention have solid and liquid phase transitions with temperature changes.

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Abstract

The invention belongs to the technical field of biomedical materials, and relates to a porous nano-scale soft colloid and a method for transporting cells or tissue cultures by using the porous nano-scale soft colloid. Isopropylacrylamide is used as a main body of the porous nano-scale soft colloid, and after molecule grafting, polymerization with 2-mercaptobenzoic acid and lauryl sodium sulfate iscarried out; grafted molecules comprise acrylic acid, methacrylic acid, methylene succinic acid, hydroxysuccinic acid, hydroxyethyl methacrylate, dimethyl methacrylate amino ethyl ester or methoxypolyethylene glycol; the porous nano-scale soft colloid is mixed with cells or tissue cultures to be transported, heating and inoculating of an obtained mixture to a cell culture plate, a culture bottleor other containers is carried out for transportation, cooling and centrifuging are carried out after transportation, and harvesting of complete cells or tissue cultures is realized again. Effective physical support, fixation and physical protection are provided in the transportation process of cells or tissue cultures, damage of collision and shearing force in the transportation process to the cells or tissue cultures is reduced, and the requirement for long-distance transportation of the cells or tissue cultures is met.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a porous nano-scale soft colloid used to maintain the biological activity and functionality of cells or tissue cultures during the transportation of cells or tissue cultures and the transport of cells or tissue cultures way of things. Background technique [0002] In the past 80 years, the development of drugs has undergone tremendous changes, from the era of small molecule drugs to the era of macromolecular drugs (antibody drugs), and then to the new wave that has emerged in the past 5 years - cell therapy, and to the current continuous Emerging tissue engineering treatments, etc. As of the end of 2018, there are currently 6,176 stem cell-related clinical projects underway around the world. There are 12 stem cell therapies on the market in the world, of which 11 are related to mesenchymal stem cells. At the same time, the application of tissue enginee...

Claims

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Application Information

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IPC IPC(8): C12N5/00C12N5/0775C12N5/095C08F220/54C08F220/06C08F222/02
CPCC12N5/0068C12N5/0663C12N5/0695C08F220/54C12N2533/30C12N2539/10C08F220/06C08F222/02
Inventor 崔小林刘汉石
Owner DALIAN PRACTICAL BIOTECH
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