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Group of markers for reflecting aspergillus flavus poisoning

A kind of Aspergillus flavus and marker technology, applied in the field of animal poisoning detection, can solve the problem of only a small number of samples can be analyzed accurately, and achieve the effect of reducing economic losses

Pending Publication Date: 2020-06-12
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a group of markers that reflect aflatoxin poisoning, and overcome the problems that the current methods for detecting aflatoxin poisoning can only analyze a small number of samples or have low accuracy

Method used

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  • Group of markers for reflecting aspergillus flavus poisoning
  • Group of markers for reflecting aspergillus flavus poisoning
  • Group of markers for reflecting aspergillus flavus poisoning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Select 24 four-week-old SD rats, and randomly divide them into three groups: control group, low-dose toxin group (0.025 μg / Kg AFB1), and high-dose toxin group (0.25 μg / Kg AFB1). One week later, the abdominal aortic blood of the rats was collected, and the blood was naturally coagulated at room temperature for 20 minutes, centrifuged for 20 minutes (3000 rpm), and the supernatant was collected, and the blood biochemical indicators were detected by an automatic biochemical analyzer.

[0033] data analysis:

[0034] The data were statistically analyzed using IBM SPSS22.0, MedCalc, and excel software, and the results were expressed in the form of mean ± standard deviation, as shown in Table 1:

[0035] Table 1 is the characteristics of the blood-related indicators of the experimental rats

[0036]

[0037]

[0038] * indicates that this group is significantly different from the control group at p2 , AFU, CHE, Fe and other indicators were significantly or extremely si...

Embodiment 2

[0050] Repeat the experiment in the specific embodiment, feed the 4-week-old SD rats again, and divide them into three groups, and randomly select the control group, low-dose toxin group, and high-dose toxin group for 8 weeks. Rat indicators are substituted into the radar chart middle. The result is as expected.

[0051] Such as Figure 16 Shown: put the extracted indicators of the control group into the radar chart, the area is smaller than the area of ​​mild aflatoxin poisoning, and the result is in line with expectations.

[0052] Such as Figure 17 Shown: put the extracted low-dose toxin group indicators into the radar chart, the area is larger than the area of ​​mild aflatoxin poisoning, and smaller than the area of ​​severe aflatoxin poisoning, the result is in line with expectations.

[0053] Such as Figure 18 Shown: Put the extracted high-dose toxin group indicators into the radar chart, the area is larger than the area of ​​severe aflatoxin poisoning, and the res...

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Abstract

The invention relates to a group of markers for reacting aspergillus flavus poisoning. Blood indexes are glutamic-pyruvic transaminase, glutamic oxalacetic transaminase, alkaline phosphatase, lactic dehydrogenase and cholinesterase. ALT, AST, LDH, ALP and CHE in the blood indexes are sensitive to animals poisoned by aflatoxin; a detection model is established by detecting contents of ALT, AST, LDH, ALP, CHE and the like in serum (plasma) and analyzing correlation with aspergillus flavus poisoning; the problems that the aspergillus flavus poisoning of animals cannot be found and treated early,the detection difficulty is high and the cost is high clinically at present are solved, the aspergillus flavus poisoning is found in time, treatment is conducted as soon as possible, and economic losses caused to animal husbandry are reduced.

Description

technical field [0001] The invention belongs to the field of animal poisoning detection and relates to a group of markers reflecting Aspergillus flavus poisoning. Background technique [0002] Aflatoxin (AFT) is a secondary metabolite produced by toxin-producing strains such as A. . Among them, aflatoxin B1 (AFB1) is the most important type of aflatoxin, and it is also the most toxic biological toxin found so far. It was listed as a class I carcinogen by the World Health Organization (WHO) in 1993. The detection rate of AFT in developed countries is low; in developing countries, the AFT pollution rate of crops and feed is relatively high; in my country, aflatoxins are widely distributed in central, southern and northern China, and the pollution is relatively serious, while the northeast and northwest regions are more polluted. light. Feed contaminated by mycotoxins will affect the production performance of livestock and poultry, cause various diseases, and limit the develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/52C12Q1/46C12Q1/42C12Q1/32
CPCC12Q1/52C12Q1/46C12Q1/42C12Q1/32G01N2333/904G01N2800/709
Inventor 陈媛媛张弘弢徐闯陈起良李福华李晶吴倩高颖余星潼于佳颖杨欣
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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