Weak contact sample concentration and purification method and application

A sample concentration and purification method technology, which is applied in the field of weak contact sample concentration and purification, can solve the problems of active component damage, affecting the distribution of functional groups, and no pretreatment, etc., to achieve improved accuracy, small contact surface, and concentration high rate effect

Active Publication Date: 2020-06-02
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no concentration and purification technology for this type of sample, so most of them do not carry out corresponding pretreatment, but directly detect the extracted sample
[0004] However, conventional centrifugation and electrophoresis techniques are limited in scope
The centrifuge uses the density difference of the components to separate, and the separation effect is not obvious for the components with similar density or particle size to nanoscale, and the strong relative motion will cause damage to the active components
Electrophoresis can only drive and separate charged components, and the local electric field will affect the distribution of functional groups on the surface of components, and will also cause damage to active components

Method used

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  • Weak contact sample concentration and purification method and application
  • Weak contact sample concentration and purification method and application
  • Weak contact sample concentration and purification method and application

Examples

Experimental program
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Embodiment 1

[0035] The method for concentrating and purifying the liquid sample of the present embodiment comprises the following steps:

[0036] All the following steps are carried out in an airtight chamber to isolate external water vapor and floating dust. The interior of the airtight chamber is dry air, nitrogen or inert gas, the ambient temperature inside the chamber is room temperature, and the pressure is kept in balance with the outside atmosphere through the one-way regulating valve.

[0037] Step 1, processing the original solution, the medium of the original solution is water, organic solvent or liquid metal, in the embodiment, the original solution of the influenza virus nucleic acid detection sample is pretreated.

[0038] Such as figure 1 As shown, use a micro-injection pump to drop the stock solution to be processed to the surface of the supercooled bottom plate 30 through the capillary 10 to obtain a droplet 20 on the surface of the bottom plate. The droplet 20 contains a...

Embodiment 2

[0060] The stock solution in this embodiment is a mixed nanofluid, and its main components are water (matrix medium), polystyrene particles (interfering component), and gold particles (target component).

[0061] The separation of polystyrene nanoparticles and silver nanoparticles with the same particle size of 50nm in the stock solution comprises the following steps:

[0062] Step 1, such as Figure 5 As shown, the stock solution to be processed is dropped onto the surface of the supercooled base plate 30 through a capillary 100 using a micro-syringe pump to obtain a droplet 200 on the surface of the base plate. The droplet 200 contains a plurality of polystyrene nanoparticles with the same particle diameter of 50 nm. 202 and silver nanoparticles 201, the base plate 30 is provided with a residual night capillary channel 31 communicating with the surface of the base plate 30.

[0063] The mixed nanofluid is injected through the capillary channel of the stock solution. The out...

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Abstract

The invention discloses a weak contact sample concentration and purification method and an application. The weak contact sample concentration and purification method comprises the following steps: 1,collecting a sample stock solution with internal free or suspended components, and dripping the sample stock solution onto the surface of a supercooled bottom plate to obtain liquid drops on the surface of the bottom plate; 2, performing the solidification nucleation of liquid drops on a supercooling bottom plate, wherein solidification nucleation occurs on a solid-liquid contact surface, and target components in the liquid drops are gathered at the top areas of the liquid drops due to solidification segregation; 3, controlling the temperature of the supercooling bottom plate, and pausing themovement of a solidification interface; 4, extracting a concentrate at the top of the liquid drop. Compared with the prior art, the weak contact sample concentration and purification method provided by the invention has the advantages of synchronous concentration and purification, no need of chemical solvent extraction, small contact surface between the sample and the container, no pollution, no strong physical field and no relative motion, no damage to the activity of the target component due to low-temperature operation in the whole process, and is especially suitable for small-dose sampleswith biochemical activity.

Description

technical field [0001] The invention belongs to the technical field of concentration and purification, and in particular relates to a method for concentration and purification of a weak contact sample and its application. Background technique [0002] At present, in biochemical detection, due to the low concentration of target components in the sample, the reaction signal is weak, which prolongs the detection cycle and even affects the detection effectiveness. Taking the clinical mainstream immunity and nucleic acid testing as an example, the test samples are blood and saliva extracts respectively. In the early stage of the patient's onset, the concentration of antigens, antibodies and viral DNA is very low, and due to the difficulty of diagnosis, there will often be delays in causing the disease. [0003] At present, there is no concentration and purification technology for such samples, so most of them do not carry out corresponding pretreatment, but directly detect the e...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N1/40
CPCG01N1/34G01N1/4022G01N2001/4033Y02P10/20
Inventor 赵玉刚杨英英郑平杨纯
Owner UNIV OF SHANGHAI FOR SCI & TECH
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