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Method detecting harmful compounds in plastic toy based on surface plasma resonance technology

A surface plasmon and plastic toy technology, applied in measuring devices, analyzing materials, scattering characteristics measurement, etc., can solve the problems of high cost, large amount of consumables, poor antibody stability, etc., to prolong service life, reduce detection cost, regenerate easy effect

Inactive Publication Date: 2014-01-15
王利兵
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Problems solved by technology

[0005] In order to solve the problems of sensing specificity, poor antibody stability, large amount of consumables, and high cost existing in the existing harmful compound immunoassay technology, and at the same time greatly improve the detection limit of harmful compounds, the present invention provides a surface plasmon-based Resonance instrument detection method for harmful compounds in plastic toys

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  • Method detecting harmful compounds in plastic toy based on surface plasma resonance technology

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Embodiment

[0030] Example: Two typical harmful compounds bisphenol A and ethanolamine in plastic toys are selected as the detection objects, and the specific operation steps are as follows:

[0031] (1) Immobilization of aptamers on the surface of the sensor: select the sensor chip modified by streptavidin SA, and inject 20 μL of biotin-labeled thrombin aptamer with a concentration of 30 μg / L on the surface of the plasmon sensor chip. Immobilize the thrombin aptamer;

[0032] (2) Molecular probe label coupling: the analyte is coupled with thrombin by the glutaraldehyde method, and the labeling method is selected according to the group of the analyte, including EDC / NHS method, glutaraldehyde method, etc. , such as bisphenol A, can be linked with thrombin by EDC / NHS method, and set aside;

[0033] (3) Preparation of standard solution: Weigh 10 mg of bisphenol A standard substance, dissolve it in 0.02mol / L (pH=7.4) phosphate buffer solution and dilute to 10 mL, which is the standard stock ...

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Abstract

The invention discloses a method detecting harmful compounds in a plastic toy based on a surface plasma resonance technology, belonging to the technical field of residue detection of harmful compounds. The method comprises sensing surface aptamer fixing, sample marking, sample detecting and sensing element regenerating; the same sensing chip is used for detecting the residual contents of different harmful compounds; and the method is characterized by comprising the following steps of: connecting the surface of the sensing chip with a thrombin aptamer serving as a marking molecular probe; performing a series of pretreatment on a sample, adding thrombin markers for detecting harmful compounds, mixing, and competitively binding the aptamers which correspond to meal nanoparticle-modified harmful compounds; detecting the content of thrombin by using a surface plasma resonance detector, and indirectly detecting harmful compounds; and regenerating the sensing chip which is used for detecting other harmful compounds. A general detection method with high stability, high repeatability and high sensitivity is provided for detecting harmful compounds in plastic toys, detection of different harmful compounds with the same surface plasma resonance sensing chip is realized, and high generality is achieved.

Description

technical field [0001] The invention relates to a technology for detecting harmful compounds in plastic toys. In particular, based on surface plasmon resonance technology, through specific competitive recognition of nucleic acid aptamers and unlabeled and labeled samples, using thrombin aptamer-modified sensing elements to indirectly detect harmful compounds in plastic toys. Background technique [0002] Toys have always been intimate partners of infants and young children, but they can also cause health problems. Residues of harmful compounds are invisible killers hidden in plastic toys. There are many types of harmful chemicals involved in baby toys, such as resins and stabilizers, plasticizers, colorants and other additives. The bisphenol A incident that has occurred in recent years has aroused widespread concern about harmful chemicals in plastic toys. Bisphenol A is the main raw material for the manufacture of epoxy resins, polycarbonate resins, and polystyrene resins....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N21/552
Inventor 王利兵黄仁亮苏荣欣
Owner 王利兵
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