Method for detecting biotoxins in foods based on surface plasma resonance technology

A surface plasmon and biotoxin technology, which is applied in the direction of measuring devices, scattering characteristics measurement, and material analysis through optical means, can solve the problems of poor stability, high cost, and large amount of consumables, and achieve easy regeneration, extended service life, The effect of reducing inspection costs

Inactive Publication Date: 2013-12-18
王利兵
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  • Abstract
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  • Claims
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Problems solved by technology

[0007] In order to solve the problems of chip specificity, poor stability, large amount of consumables, and high cost in the detection of biotoxins in food by the existing surface plasmon technology, and at the same time greatly improve the detection limit of biotoxins, the present invention provides a food biotoxin Surface Plasmon Resonance Detection Method of Biotoxins

Method used

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  • Method for detecting biotoxins in foods based on surface plasma resonance technology
  • Method for detecting biotoxins in foods based on surface plasma resonance technology
  • Method for detecting biotoxins in foods based on surface plasma resonance technology

Examples

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Embodiment

[0032] Embodiment: specific example Molecular probe takes protein kinase MEKI as an example, and selects the biological toxins botulinum neurotoxin and aflatoxin in two kinds of food as detection objects, and the specific operation steps are as follows:

[0033] (1) Immobilization of aptamers on the sensor surface: The sensor chip modified with streptavidin SA was selected, and 20 μL of biotin-labeled protein kinase MEKI aptamer at a concentration of 30 μg / L was introduced into the surface plasmon sensing chip. The protein kinase MEKI aptamer is immobilized on the chip;

[0034] (2) Molecular marker coupling: The analyte is conjugated and labeled with the protein kinase MEKI by the glutaraldehyde method. The labeling method is selected according to the different groups of the analyte, including EDC / NHS method, glutaraldehyde method, etc. For example, botulinum neurotoxin can be connected with protein kinase MEKI by EDC / NHS method, and it is ready for use;

[0035](3) Standard...

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Abstract

The invention discloses a method for detecting biotoxins in foods based on a surface plasma resonance technology, which belongs to the technical field of food safety hazard factor detection. The method comprises sensing surface aptamer fixing, sample marking, sample detecting and sensing element regenerating; the same sensing chip is used for detecting the contents of different biotoxins by using the same sensing chip; and the method is characterized by comprising the following steps of: connecting aptamers which correspond marking molecular probes to the surface of the sensing chip; performing a series of treatment on a food sample, adding biotoxins marked by using molecular probes, mixing, and competitively binding the aptamers which are modified by metal nanoparticles and correspond to the biotoxins; detecting the quantity of the molecular probes by using a surface plasma resonance detector to indirectly detect biotoxins; and regenerating the sensing chip for detecting other biotoxins. Compared with the conventional antibody, the aptamer for identifying molecules on the sensing chip has the characteristics of high stability, quick response and easiness and convenience for operating; and the service life the chip is prolonged greatly, the detecting sensitivity is greatly improved, and detection of the various biotoxins in foods is realized by using plasma resonance sensing chips on the same surface.

Description

technical field [0001] The invention relates to a technology for detecting biological toxins in food. In particular, based on surface plasmon resonance technology, through specific competition recognition between nucleic acid aptamers and unlabeled and labeled samples, and the use of molecular probe aptamer-modified sensing elements to indirectly detect biological toxins in food. Background technique [0002] Biotoxins in food seriously endanger people's health, which has aroused widespread concern. There are many kinds of biological toxins. Common biological toxins include botulinum neurotoxin in canned food and sealed pickled food, aflatoxin in nuts and ochratoxin in grains, etc. They vary and can cause nerve paralysis, cancer, liver toxicity, kidney damage, and more. Biological toxins may be produced or infected during food production, processing, transportation and consumption, resulting in serious food safety incidents. Therefore, it is extremely important to realize...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N21/552
Inventor 王利兵苏荣欣韩伟
Owner 王利兵
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