Method for detecting pesticide and veterinary medicament residues in foods based on surface plasma resonance technology
A surface plasmon and veterinary drug residue technology, which is applied in measuring devices, scattering characteristics measurement, and material analysis through optical means, can solve the problems of expensive counterfeiting, poor stability of antibodies, and large amount of consumables, etc., to reduce detection costs and regenerate Easy, long life effect
Inactive Publication Date: 2014-01-15
王利兵
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[0005] In order to solve the problems of chip specificity, poor antibody stability, large amount of consumables, and expensive counterfeiting in the detection of agricultural and veterinary drug residues in the existing surface plasmon technology, and to greatly improve the detection limit of agricultural and veterinary drug residues, the present invention provides a Method for detecting various pesticide and veterinary drug residues in food using surface plasmon resonance sensor
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[0030] Example: Two typical veterinary drug residue kanamycin and pesticide residue organophosphorus analogue PAPMP are selected for detection. The specific operation steps are as follows:
[0031] (1) Aptamer immobilization on the surface of the sensor: select the sensor chip modified by streptavidin SA, pass 20 μL of biotin-labeled lysozyme aptamer with a concentration of 30 μg / L, on the surface of the plasmon sensor chip Immobilize the lysozyme aptamer;
[0032] (2) Molecular probe label coupling: the analyte is coupled with lysozyme by the glutaraldehyde method, and the labeling method is selected according to the group of the analyte, including EDC / NHS method, glutaraldehyde method, etc. , such as kanamycin, can be linked to lysozyme by glutaraldehyde method, ready for use; or directly purchase kanamycin-lysozyme conjugate for use;
[0033] (3) Preparation of standard solution: Weigh 10 mg of kanamycin standard substance, dissolve it in 0.02mol / L (pH=7.4) phosphate buffe...
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The invention discloses a method for detecting pesticide and veterinary medicament residues in foods based on a surface plasma resonance technology, which belongs to the technical field of food safety hazard factor detection. The method comprises sensing surface aptamer fixing, sample marking, sample detecting and sensing element regenerating; the same sensing chip is used for detecting the contents of different pesticide and veterinary medicament residues by using the same sensing chip; and the method is characterized by comprising the following steps of: preparing lysozymes serving as marking molecular probes, and connecting lysozyme aptamers to the surface of the sensing chip; performing a series of treatment on a food sample, adding lysozyme markers for pesticide and veterinary medicament residual components, mixing, and competitively binding the aptamers which are modified by metal nanoparticles and correspond to the pesticide and veterinary medicament residual components; detecting the quantity of the lysozymes by using a surface plasma resonance detector to indirectly detect pesticide and veterinary medicament residues; and regenerating the sensing chip for detecting other pesticide and veterinary medicament residues. A general detection method with high stability, high repeatability and high sensitivity is provided for the detection of pesticide and veterinary medicament residues, and detection of various pesticide and veterinary medicament residues through plasma resonance sensing chips on the same surface is realized.
Description
technical field [0001] The invention relates to a technology for detecting pesticide and veterinary drug residues in food. In particular, based on surface plasmon resonance technology, through the specific competitive recognition of nucleic acid aptamers and unlabeled and labeled samples, the method of indirect detection of agricultural and veterinary drug residues in food using lysozyme aptamer-modified sensing elements. Background technique [0002] In recent years, food safety issues have become the focus of people's livelihood, and pesticide and veterinary drug residues are the primary factor restricting food safety. Organophosphorus pesticides, one of the traditional three types of pesticides, are used in large amounts and widely used, and the problem of residues has attracted much attention. Acetamiprid is unavoidably present in fruits and vegetables because it is a spectrum insecticide. The chemical functional group triphenylmethane in malachite green can cause canc...
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IPC IPC(8): G01N21/55G01N21/552
Inventor 王利兵黄仁亮于艳军
Owner 王利兵
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