Method for identifying honeysuckle and lonicerae flos by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry
A technology of high performance liquid chromatography and liquid mass spectrometry, which is applied in the field of identification of varieties of Chinese herbal medicines, can solve the problems of insufficient speed of inspection, complicated inspection methods, and many influencing factors, and achieve high sensitivity, strong specificity, and improved accuracy Effect
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Embodiment 1
[0039] Embodiment 1 establishes detection method
[0040] 1. Instruments and materials
[0041] 1.1 Instrument
[0042] TSQ-TUANTOM liquid mass spectrometry instrument (THERMO FISHER company), electrospray ionization source (ESI) mass spectrometer detector, waters C18 chromatographic column (2.1×10mm, 1.7μm);
[0043] Agilent 1260 Quaternary HPLC, Agilent G4212B Diode Array Detector, Agilent Eclipse XDB-C 18 Chromatographic column (250mm×4.6mm, 5μm);
[0044] KH500B ultrasonic cleaner (Kunshan GRAD), clear D24UV ultrapure water system (Millipore China).
[0045] 1.2 Materials and reagents
[0046] Acetonitrile (Fisher Scientific) was of chromatographic grade, water was of primary ultrapure water, absolute ethanol (COMIO) and other reagents were of analytical grade; formic acid and ammonium formate were of mass spectrometry grade.
[0047] Broken strychnic acid reference substance was purchased from Shanghai Shidande Standard Technology Service Co., Ltd., batch number: 600...
Embodiment 2
[0143] Embodiment 2 verification experiment
[0144] 1. The content of strychnic acid and chlorogenic acid in honeysuckle from different origins and batches
[0145] Each batch of honeysuckle from Hebei Province, Henan Province and Shandong Province was taken as the test sample and tested according to the detection conditions of the method described in Example 1, and the obtained detection results are shown in Table 13.
[0146] Table 13 Detection results of each batch of honeysuckle in Hebei Province, Henan Province and Shandong Province
[0147]
[0148]
[0149] 2. The content of strychnic acid and chlorogenic acid in different origins and batches of mountain silver flowers
[0150] Each batch of Sanyinhua in Guizhou Province, Hunan Province, Guangxi Province, Guangdong Province, and Sichuan Province was used as the test sample and tested according to the detection conditions of the method described in Example 1. The obtained detection results are shown in Table 14. ...
Embodiment 3
[0162] Example 3 mixed sample (honeysuckle and mountain silver flower mass ratio is 1:1 mixed) determination
[0163] Mixed sample preparation: according to Table 15, take equal weights of Flos Lonicerae and Flos Lonicerae respectively, pulverize, mix, as the test sample, refer to the determination of chlorogenic acid, cychnicic acid, and diapsus saponin B under the item of Example 1 The method was used to determine and calculate the content ratio of chlorogenic acid and stanchanic acid and the content of Dipsacus saponin B. The results are shown in Table 16.
[0164] Table 15 Composition table of mixed samples of honeysuckle and mountain silver flower (mass ratio is 1:1)
[0165] Mixed sample number Honeysuckle variety name Mountain silver flower variety name composition mass ratio 1 The first batch of first class in Henan 3 Guizhou gray felt wool-1 1:1 2 The first batch of first class in Henan 14 Hunan Longhui Shanyinhua 1:1 3 The firs...
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