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Bacillus velezensis MES861, and application and product thereof

A bacillus and product technology, applied in the field of microorganisms, can solve problems such as no Bacillus velesi blight, and achieve the effects of reducing rice blight, improving yield and quality, and promoting stable production.

Active Publication Date: 2020-05-19
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the control of rice blight by Bacillus velezensis

Method used

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  • Bacillus velezensis MES861, and application and product thereof
  • Bacillus velezensis MES861, and application and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The isolation and identification of embodiment 1 bacterial strain

[0031] The soil of the pinellia planting base of Sinopharm Group was diluted, cultured by marking, and cultured at 28°C for 24 hours. After five times of purification, the slant strain was made, and 2 slants were picked and sent to the microbial quality inspection agency of the Ministry of Rural Agriculture for identification. Bacillus velezensis, stored in refrigerator at 4°C and -80°C.

[0032] After morphological observation, microscopic examination, enzymatic analysis and carbohydrate utilization experiment, combined with 16S rDNA sequencing comparison, it was identified as Bacillus Velesi and named as MES861. The 16S rDNA gene sequence is shown in SEQ ID NO.1. The strain is a Gram-positive bacterium with oval endospores belonging to the genus Bacillus.

[0033] The results of some physiological and biochemical reactions are: catalase positive, catalase positive, lipase positive, gelatin liquefacti...

Embodiment 2

[0037] The preparation of embodiment 2 microbial bacterial agents

[0038] (1) First-level seed culture: Bacillus Velez MES861 activated on the inclined surface of the test tube was cultured in an ampey bottle, and the ampey bottle medium was NB medium.

[0039] The formula of NB medium is: 8-13 grams of peptone, 2-5 grams of beef extract, 3-5 grams of sodium chloride, 19.5 grams of agar, 1000 ml of ultra-pure water, and the pH value is 7.0-7.5. The culture conditions are: temperature 22°C-30°C, culture for 24h.

[0040] (2) Fermentation: at 30m 3 The expanded fermentation tank is equipped with 20L liquid fermentation medium, sterilized by moist heat at 115°C-122°C for 35-40min, then cooled to 35°C, inoculating the primary seeds in the liquid fermentation medium, and controlling the pressure of the tank at 0.02Mpa-0.07Mpa , and cultured at 35°C for 26h.

[0041] The raw materials and dosage of the liquid fermentation medium are: 2% glucose, 0.5% yeast extract powder, 0.75% ...

Embodiment 3

[0045] Example 3 Antibacterial test of Bacillus velei MES861 on rice blight

[0046] (1) The source of the tested bacteria

[0047] R. solanum blight BYJ-03 was isolated from the diseased rice seeds of Erdaohe Farm in Heilongjiang. It was cultivated, isolated and purified by the R&D personnel of the company. It was finally identified as Fusarium asiaticum by 16S rDNA and identified as Fusarium asiaticum by pathogenicity test. Strong pathogenicity.

[0048] (2) Plate antagonism experiment

[0049] Prepare a PDA solid plate, use a sterile inoculation needle to pick a small amount of Fusarium graminearum and place it in the middle of the petri dish, burn the red inoculation needle, wait for the inoculation needle to cool down, dip a small amount of Bacillus Velez MES861 and inoculate it at a distance from the inoculation pathogenic bacteria Around the 2.0 cm spot, the treatment that was not inoculated with Bacillus Veles and only inoculated with Fusarium graminearum was set as ...

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Abstract

The invention provides bacillus velezensis MES861, and an application and product thereof, and relates to the technical field of microorganisms. The bacillus velezensis MES861 is preserved in China General Microbiological Culture Collection Center on Dec.3,2018, and the preservation number is CGMCC NO.16857. The strain has an obvious antagonistic effect on fusarium graminearum, can prevent or reduce occurrence of rice seedling blight, promote stable yield, increase income, and improve and increase the yield and quality of rice.

Description

technical field [0001] The invention relates to the technical field of microbes, and specifically provides Bacillus Velez MES861 and its application and products. Background technique [0002] Rice is the largest food crop in my country, but there are still problems in some areas where the growth of seedlings is poor, mainly manifested as sick and weak seedlings. Among them, under the conditions of dry seedling raising, blight has become the biggest obstacle to dry breeding and sparse planting technology. Under normal conditions, the incidence of blight is about 15%. Because of the causes of climate, management, etc., the devastating incidence is also frequent. have happened. The disease is due to the influence of various unfavorable environmental factors, which leads to the reduction of the disease resistance of the seedlings, so that they are easily invaded by Fusarium, Rhizoctonia solani and other pathogens to cause disease. [0003] Rice blight is a soil-borne disease,...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 顾欣燕肖培英赵晓琛刘金龙
Owner 天津开发区坤禾生物技术有限公司
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