Rabdosia japonica glycoprotein XPS10-1, preparation method therefor and use of Rabdosia japonica glycoprotein XPS10-1
A kind of blue calyx tea vegetable and glycoprotein technology, which is applied in the fields of peptide preparation method, peptide/protein composition, chemical instruments and methods, etc., can solve the problems of tissue expression and substrate specificity differences, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation Embodiment 1
[0034]Materials: 5.0 kg of blue calyx fragrant tea (batch number: SPHTCMXCC01) was obtained from Fengyang, Anhui (collected on November 20, 2015), DEAE Sepharose Fast Flow anion exchange resin and Superdex series molecular sieve gel columns were purchased from General Electric GE Healthcare; Pullulan P-82 standard set: P-5, P-10, P-20, P-50, P-100, P-200, P-400, P-800, Shodex; water is Ultrapure water (self-made in the laboratory); ascorbic acid, tryptophan, methylene blue, Catalase, dimethylaminobenzaldehyde, trichloroacetic acid, monosaccharide standard (D-glucose, D-arabinose, L-fucose , L-rhamnose, D-mannose, D-xylose, D-galactose) and trifluoroacetic acid were purchased from SIGMA Company; IDO enzyme was purchased from BPS Company, and 384-well compound plates were purchased from Nunc Company; Hela cells were purchased from Provided by Professor Li Erguang of Nanjing University, it was purchased from ATCC (product number CCL-2); DMEM, sodium pyruvate, L-Glutamine, non-ess...
preparation Embodiment 2
[0041] Taking different batches of S. japonica (batch number: SPHTCMXCC02), the extraction and separation and purification methods of Preparation Example 1 were repeated to obtain the S. sativa polysaccharide XPS10-1 of Preparation Example 2.
preparation Embodiment 3
[0043] Taking different batches of S. japonica (batch number: SPHTCMXCC03), the extraction and separation and purification methods of Preparation Example 1 were repeated to obtain the S. sativa polysaccharide XPS10-1 of Preparation Example 3.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com