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Salt-tolerant, heterotrophic and aerobic nitrifying bacterium strain, culture method, bacterial liquid and application

A nitrifying bacteria strain and heterotrophic nitrification technology, applied in the field of salt-tolerant heterotrophic aerobic nitrifying bacteria strains, can solve problems such as poisoning and achieve good market application prospects

Active Publication Date: 2020-05-08
北京翰祺环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ammonia nitrogen wastewater is also toxic to fish and some organisms

Method used

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  • Salt-tolerant, heterotrophic and aerobic nitrifying bacterium strain, culture method, bacterial liquid and application
  • Salt-tolerant, heterotrophic and aerobic nitrifying bacterium strain, culture method, bacterial liquid and application
  • Salt-tolerant, heterotrophic and aerobic nitrifying bacterium strain, culture method, bacterial liquid and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The isolation and enrichment screening steps of heterotrophic nitrifying strains are as follows:

[0040] (1) Activated sludge was collected from China Petroleum Dagang Petrochemical Company in Tianjin, and the sludge sample was inoculated into sterilized nitrification medium by dilution and plate coating method. The initial NH 4 + -N concentration is 100 mg / L, and the formula of nitrification medium is as follows (g / L): ammonium chloride 0.3, sodium acetate 1, disodium hydrogen phosphate 0.2, potassium chloride 0.1, sodium chloride 10, magnesium sulfate 0.05, and 1 mL Trace element solution, pH 7.0, cultivated on a shaker at 30°C to obtain isolated strains;

[0041] (2) Place the strain isolated in (1) in the culture medium for NH 4 + -N removal experiment, NH 4 + The strain with the highest removal rate of -N was the desired strain.

Embodiment 2

[0043] Molecular biological identification of strains

[0044] 30 strains were screened from the activated sludge, and the HQXH-21 strain with the best ammonia nitrogen removal effect was selected for 16S rRNA identification, and it was identified as Pseudomonas by using 16S rRNA ( Pseudomonas sp. ), and named Pseudomonas sp. HQXH-21, the single colony of this strain in LB medium is regular round, moist, yellow in color, opaque, and viscous. See the appendix for the sequence list in the 16S rRNA identification of the bacterial strain of the present invention.

Embodiment 3

[0046] Pseudomonas sp. The cultivation method of HQXH-21

[0047] The above-mentioned isolated bacterial strains are cultivated in a nitrifying medium, which consists of: 0.3g / L ammonium chloride, 1g / L sodium acetate, 0.2g / L disodium hydrogen phosphate, 0.1g / L potassium chloride, and 10g sodium chloride / L, magnesium sulfate 0.05g / L and 1mL trace element solution; culture temperature is 30°C; pH is 7;

[0048] Inoculate 5% of the inoculum in the nitrification medium, culture at 37°C and 150 r / min on a shaker; every 48 h, replace with fresh heterotrophic nitrification medium at a volume ratio of 10%; continuously culture for more than 30 h.

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Abstract

The invention discloses a salt-tolerant, heterotrophic and aerobic nitrifying bacterium strain, and provides a culture method of the strain, a bacterial liquid containing the strain as well as an application mode of the strain in removal of ammonia nitrogen in wastewater. The strain is separated from activated sludge. Through identification, the strain is Pseudomonas sp. In a liquid culture mediumtaking glucose as a carbon source and ammonium chloride as a unique nitrogen source, the strain has a removal rate of ammonia nitrogen up to 85% or above and a removal rate of total nitrogen up to 70%, and has an efficient nitrogen removal function. The strain provides a good microbial material for biological nitrogen removal and treatment of polluted water with high salt and high ammonia nitrogen, and has better market application prospects.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, more specifically, to a salt-tolerant heterotrophic aerobic nitrifying bacterial strain, a culture method, a bacterial solution and an application. Background technique [0002] With the development of industry and agriculture and the improvement of people's living standards, the discharge of nitrogen-containing compound wastewater has increased sharply, and it has become a major source of environmental pollution and has attracted much attention. In recent years, nitrogen pollution has become a major hidden danger to the safety of my country's surface water environment. The discharge of a large amount of ammonia nitrogen wastewater into the water not only causes eutrophication of the water body, but also causes the water body to be black and smelly, and the difficulty and cost of water supply treatment increase, and even harm the human population and organisms. produce toxic e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/16C12N1/20C12N1/205C12R2001/38
Inventor 王晓阳汪德罡谢晓朋
Owner 北京翰祺环境技术有限公司
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