A method for constructing uplc characteristic map of magnolia formula granules and its component content determination

A technology of formula granules and characteristic maps, which is applied in the directions of measuring devices, material separation, and analysis of materials, can solve the problems of poor water solubility, low polarity, and lack of specificity, and achieve the effect of stable method, high precision, and effective evaluation

Active Publication Date: 2022-04-12
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality control mode of magnolia formula granules still continues the traditional chemical control idea, and magnolia is mostly used as the quantitative index. However, the active ingredients of lignans represented by magnolia are less polar and have poor water solubility, so , the transfer rate of such chemical components from decoction pieces to formula granules is not high, and the separate determination of lignan components such as magnolia is not enough for the quality control of the production process of magnolia formula granules
In addition, a single quantitative analysis of chemical components is difficult to fully reflect the internal quality of formula granules, and it also lacks specificity. Chromatographic fingerprints / characteristics of traditional Chinese medicine can provide more comprehensive and rich quantitative and semi-quantitative information
Due to the shortcomings of low column efficiency, limited chromatographic separation ability and long analysis time, the traditional high performance liquid chromatography (HPLC) fingerprint / characteristic spectrum has been difficult to adapt to the research of complex systems of traditional Chinese medicine.

Method used

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  • A method for constructing uplc characteristic map of magnolia formula granules and its component content determination
  • A method for constructing uplc characteristic map of magnolia formula granules and its component content determination
  • A method for constructing uplc characteristic map of magnolia formula granules and its component content determination

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Construction of characteristic map of magnolia formula granules

[0043] 1. Main instruments, reagents and reagents

[0044] 1.1 Main instruments: Waters H-Class ultra-high performance liquid chromatography (Waters Corporation); YMC Triart C18 (100mmx2.1mm, 1.9μm) chromatographic column, ME204E 1 / 10,000 analytical balance (Mettler-Toledo Corporation ); XP26 millionth analytical balance (Mettler-Toledo); JJ600 electronic balance (Changshu Shuangjie Testing Instrument Factory); KQ500D CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); HWS28 constant temperature Water bath (Shanghai Yiheng Technology Co., Ltd.); Milli-Q Direct ultrapure water system (Merck Co., Ltd.).

[0045] 1.2 Main reagents: ethanol (Xilong Science Co., Ltd., analytically pure); methanol (Xilong Science Co., Ltd., analytically pure); phosphoric acid (Tianjin Kemiou Chemical Reagent Co., Ltd., chromatographically pure); acetonitrile (Merck Co., Ltd. company, chromatographically pure); t...

Embodiment 2

[0102] The method for determining the content of magnolia formula granules comprises the following steps:

[0103] (1) Draw the reference substance solution and the magnolia formula granule sample solution to be tested and inject them into the ultra-high performance liquid chromatograph, and measure the corresponding peak areas.

[0104] (2) Calculated by the external standard method, that is.

[0105] The detection component is magnolin.

[0106] 1. Instruments, reagents and reagents

[0107] 1.1 Main instruments: Waters ultra-high performance liquid chromatography (Waters Corporation); Thermo ultra-high performance liquid chromatography (Thermo Fisher Scientific Co., Ltd.), YMC Triart C18 (2.1×100mm, 1.9μm) column, Waters AcquityHSS T3 C18 (2.1×100mm, 1.8μm) column, Waters Acquity BEH C18 (2.1×100mm, 1.7μm) chromatographic column, one-thousandth balance (ME204E, Mettler-Toledo), one-hundred-thousandth Balance (ABT220-5DM, Guangzhou Dexiang Technology Co., Ltd.), millionth...

Embodiment 3

[0202] The method for determining the content of magnolia formula granules comprises the following steps:

[0203] (1) Draw the reference substance solution and the magnolia formula granule sample solution to be tested and inject them into the ultra-high performance liquid chromatograph, and measure the corresponding peak areas.

[0204] (2) Calculated by the external standard method, that is.

[0205] The detection component is magnolanine.

[0206] 1. Instruments, reagents and reagents

[0207] 1.1 Main instruments: Thermo vanquish UPLC ultra-high performance liquid chromatography (Thermo Fisher Scientific Co., Ltd.), Waters Acquity HSS T3 (column length is 100mm, inner diameter is 2.1mm, particle size is 1.8μm) chromatographic column, WatersCortecs T3 (Column length is 100mm, inner diameter is 2.1mm, particle size is 1.6μm) chromatographic column, Waters ACQUITY UPLC BEHC18 (column length is 100mm, inner diameter is 2.1mm, particle size is 1.7μm) chromatographic column, t...

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Abstract

The invention relates to a method for constructing UPLC characteristic map of magnolia formula granules and determination of its component content. The method for establishing the UPLC characteristic map of magnolia formula granules comprises the following steps: (1) Precisely weighing magnolia formula granules to prepare magnolia formula granules The test solution; (2) The magnolia formula granule test solution was analyzed by ultra-high performance liquid chromatography, and the UPLC characteristic spectrum of the magnolia formula granule was obtained. The characteristic map of magnolia formula granules constructed by the present invention fully demonstrates the chemical composition characteristics of magnolia formula granules. The characteristic map constructed by the present invention comprehensively reflects the characteristic peak information of samples, and the method is stable, with high precision and good reproducibility The present invention controls the quality of magnolia formula granules from three angles of UPLC characteristic map, magnolanin content determination and magnolia alkali content determination, and can more effectively evaluate the quality of magnolia formula granules.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicinal materials, and specifically discloses a method for constructing a UPLC characteristic map of magnolia magnolia formula granules and the determination of its component contents. Background technique [0002] Magnolia magnolia is the dried flower bud of Wangchun flower, Yulan Magnolia or Wudang Magnolia of Magnoliaceae. It has the effect of dispelling wind and cold and dredging the nose. , listed as the top grade, and all herbal medicines have been recorded in the past dynasties. Modern chemical and pharmacological studies have shown that Magnolia magnolia contains active ingredients such as volatile oil, lignans, flavonoids, and alkaloids, and has pharmacological effects such as anti-inflammatory, anti-allergic, blood pressure-lowering, and excitatory uterus. It is mostly used clinically to treat rhinitis and sinusitis. . [0003] The clinical use of traditional...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/86
CPCG01N30/88G01N30/06G01N30/34G01N30/8679G01N30/8686G01N2030/047G01N2030/884
Inventor 李振雨何嘉莹魏梅孙冬梅周湘媛潘礼业陈向东程学仁
Owner GUANGDONG YIFANG PHARMA
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