A method for constructing celosia hplc characteristic fingerprint and determination of flavonoids content

A technology of characteristic fingerprint and construction method, which is applied in the directions of measuring device, material separation, and analysis of materials, etc., can solve the problems of single fingerprint research of cockscomb, no research on multiple components, incomplete evaluation indicators, etc., and achieve good quality and method. Stable and accurate results

Active Publication Date: 2022-03-29
GUANGDONG YIFANG PHARMA
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  • Description
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Problems solved by technology

[0003] At present, there are few reports on the quality research of celosia. Most of the literatures only study a single fingerprint of cockscomb, and do not study its multiple components, which has great limitations. So far, there is no single A chromatographic system can be used to simultaneously determine the fingerprints that can reflect the characteristic information of the cockscomb medicinal material and the method of simultaneously determining its flavonoids. This problem needs to be solved urgently.
The composition of traditional Chinese medicine is complex, and it is not comprehensive to use the content of a few compounds as the quality evaluation index

Method used

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  • A method for constructing celosia hplc characteristic fingerprint and determination of flavonoids content
  • A method for constructing celosia hplc characteristic fingerprint and determination of flavonoids content
  • A method for constructing celosia hplc characteristic fingerprint and determination of flavonoids content

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Embodiment 1

[0039] The Construction of Characteristic Fingerprint of Celosia Cockscomb

[0040] Main instruments: Waters ARC high performance liquid chromatography (Waters, USA); Waters HSS T3 chromatographic column (4.6mm×250mm, 5μm); ME204E one-ten-thousandth balance (Mettler-Toledo, USA) ); XP26 millionth balance (Mettler-Toledo, USA); Milli-Q Integral 5 ultrapure water machine (Merck Millipore, Germany); FW100 Chinese herbal medicine grinder (Tianjin Taisi Special Instrument Co., Ltd.);

[0041] The cockscomb medicinal materials are obtained from different Chinese herbal medicine markets and pharmacies across the country.

[0042] Table 1 Sources of medicinal materials of Celosia celosia

[0043]

[0044] Main reagents: kaempferol (kaempferol) (batch number: 110861-201611; source: China Institute for Food and Drug Control; content: 95.5%); isorhamnetin (batch number: 110860-201611; source: China Food and Drug Administration Research Institute; content: 99.8%); kaempferol-4'-O-me...

Embodiment 2

[0076] Determination of the content of flavonoids in cockscomb medicinal materials, the measurement steps are as follows:

[0077] (1) Draw mixed reference substance solutions of different concentrations and inject them into a high-performance liquid chromatograph to obtain HPLC characteristic fingerprints;

[0078] (2) inject the cockscomb sample solution to be tested into the high-performance liquid chromatograph, measure the corresponding peak area, take the injection concentration as the abscissa, and take the peak area as the ordinate to draw a standard curve to determine the linear regression equation;

[0079] (3) Calculated by the linear regression equation, that is.

[0080] Preparation of sample solution of cockscomb to be tested

[0081] Take 0.2g of the cockscomb sample powder to be tested, accurately weigh it, put it in a stoppered conical flask, add 50ml of the mixed solution precisely, weigh it, reflux in a boiling water bath for 60 minutes, let it cool, weigh ...

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Abstract

The invention relates to a method for constructing an HPLC characteristic fingerprint of cockscomb and a determination of the content of flavonoids. The method for establishing the HPLC characteristic fingerprint of the cockscomb medicinal material comprises the following steps: (1) accurately weighing the cockscomb medicinal material to prepare the cockscomb test solution; Chromatographic analysis, obtain the HPLC characteristic fingerprint of cockscomb; flavonoids content determination method is: (1) draw the mixed reference substance solution of different concentration and inject high-performance liquid chromatograph, take the sample concentration as the abscissa, take the peak area as Draw a standard curve on the ordinate to determine the linear regression equation; (2) inject the cockscomb sample solution into the high-performance liquid chromatograph to measure the corresponding peak area; (3) calculate through the linear regression equation to obtain; the method precision, The stability and reproducibility are better, and the quality of Celosia celosiae can be better evaluated.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicinal materials, and specifically discloses a method for constructing an HPLC characteristic fingerprint of cockscomb and a determination of the content of flavonoids. Background technique [0002] Celosia cristata L. is the dry inflorescence of Celosia cristata L. in the family Amaranthaceae. Sweet and astringent in taste, returns to liver meridian, enters blood system. "Compendium of Materia Medica" contains: "Cockscomb can treat hemorrhoids and bleeding, red and white diarrhea, metrorrhagia, red and white discharge, divided into red and white." Cockscomb originated in Africa, America, and tropical Asia. It was introduced into my country in the Tang Dynasty, and it has been widely planted in the Ming Dynasty. Now it is planted all over the country. [0003] At present, there are few reports on the quality research of cockscomb, and most of the literatures only stud...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李国卫魏梅何民友吴淑珍吴文平陈向东程学仁孙冬梅
Owner GUANGDONG YIFANG PHARMA
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