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Construction method and identification method of uplc characteristic map of a kind of herb material and standard decoction of Desmodium glabra

It is a technology of broad money grass and characteristic maps, which is applied in the direction of material separation, analysis materials, instruments, etc. It can solve the problems of limited separation ability, long time, low sensitivity, etc., and achieve the effect of stable specificity and stable baseline

Active Publication Date: 2022-03-29
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the characteristic map of Desmodium sativa in the literature all adopts high performance liquid chromatography (HPLC method). This method takes a long time, has low sensitivity and limited separation ability, and can no longer solve the practical problems in the process of complex traditional Chinese medicine analysis.
[0004] Decoctions are mostly used in clinical practice. The content of active ingredients in decoctions directly affects the clinical effectiveness of traditional Chinese medicine. Decoctions are mainly water-soluble components. Currently, the research on the fingerprints / characteristics of Desmodium spp. Medicinal materials and some fat-soluble ingredients, while ignoring the water-soluble active ingredients in the decoction, it is difficult to reflect the clinical effect of Desmodium sativa, lacking a certain spectrum-effect relationship, and cannot reflect the material basis characteristics of clinical decoctions

Method used

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  • Construction method and identification method of uplc characteristic map of a kind of herb material and standard decoction of Desmodium glabra
  • Construction method and identification method of uplc characteristic map of a kind of herb material and standard decoction of Desmodium glabra
  • Construction method and identification method of uplc characteristic map of a kind of herb material and standard decoction of Desmodium glabra

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for constructing UPLC characteristic map of S. stylifolia medicinal material or standard decoction of S. stylii.

[0041] Main instruments: Waters ACQUITY UPLC H-Class ultra-high performance liquid chromatography (Waters Corporation, the United States); Waters ultra-high performance liquid chromatography time-of-flight high-resolution mass spectrometry system (Waters Corporation, the United States); WatersCortecs T3 Chromatographic column (4.6mm×250mm, 5μm); UNIFI 1.8 workstation data processing system (Waters, USA); MS105DU 1 / 100,000 balance (Mettler-Toledo, USA); AL104 ten thousandth balance One of the balance (Mettler - Toledo, the United States); KQ-500DE ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd.).

[0042] 18 batches of information on the origin of Herbs sativa.

[0043] Table 1 Information table of origin of 18 batches of Herbs sativa

[0044]

[0045] main reagent

[0046] Table 2 Summary of Reagent Information

[0047] ...

Embodiment 2

[0112] A kind of identification method of Herba Sinensis Herbs medicinal material, the steps are as follows:

[0113] (1) Precisely weigh the Desmodium sativa medicinal material to be identified, and prepare the sample solution of the Desmodium sativa medicinal material to be identified;

[0114] (2) Absorb the sample solution of the Herba Scutellariae to be identified, inject it into an ultra-high performance liquid chromatograph for analysis, and obtain the UPLC spectrum of the Herba Scutellariae to be identified;

[0115] (3) Compare the UPLC characteristic spectrum of the herb to be identified with the UPLC characteristic spectrum of the constructed Herba Desmodium Rhododendron; if the UPLC characteristic spectrum of the two is consistent, it is a qualified medicinal material for the preparation of clinical standard decoction; If the UPLC characteristic spectra of the two are inconsistent, it is an unqualified medicinal material for preparing clinical standard decoction. ...

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Abstract

The present invention relates to a construction method and identification method of the UPLC characteristic map of the medicinal material of S. stylifolia and the standard decoction of S. stylifolia. The construction method of the UPLC characteristic map of the s. Herbal materials, Desmodium sativa standard decoction freeze-dried powder to prepare the test solution of Herbs sativa, Desmodium sativa standard decoction; Chromatographic analysis, obtain the UPLC characteristic spectrum of Herba Desmodium Desmodium and Desmodium Desmodium Standard Decoction. The characteristic spectrum can more comprehensively reflect the characteristics of the medicinal material of Scutellaria sativa, and the method is accurate and reliable, easy to operate and good in reproducibility. To provide a rapid, accurate and objective method for the identification of S.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicines, and in particular relates to a construction method and identification method of a UPLC characteristic map of a herba stylifolia medicinal material and a standard decoction of herba stylifolia. Background technique [0002] The traditional Chinese medicine Desmodium styracifolium is the dry aerial part of Desmodium styracifolium (Osb.) Merr., a leguminous plant. It is sweet, light, and cool in nature. It belongs to the liver, kidney, and bladder meridian. Indications for urinary tract infection, urinary calculus, nephritis edema, cholecystitis, gallstones, icteric hepatitis, malnutrition in children, carbuncle, etc. The main active ingredients of Guangshencao are flavonoids, polysaccharides, saponins, alkaloids, etc. It is a commonly used Chinese medicinal material recorded in the "Chinese Pharmacopoeia" and has strong clinical application value. [0003] The 2015 edition...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李振雨周湘媛杨敏娟潘礼业林碧珊朱德全陈向东魏梅程学仁孙冬梅
Owner GUANGDONG YIFANG PHARMA
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