Preparation method of protective liquid containing feline panleukopenia yolk antibodies

A leukopenia and yolk antibody technology, applied to medical preparations containing active ingredients, antibodies, medical preparations with no active ingredients, etc., can solve the problems of short storage period, inconvenient long-distance transportation, passive application, etc., and achieve The method is simple, guarantees the quality of antibodies, and promotes the effect of digestion

Inactive Publication Date: 2020-04-28
TIANJIN HLINTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Egg yolk antibody has the advantages of stable properties, strong specificity, low preparation cost, and oral safety. It has been widely used in the prevention and treatment of poultry and livestock infectious diseases. The application is relatively passive. In view of this, the present invention helps the yolk antibody to solve some drawbacks of the prior art from the perspective of the protective solution

Method used

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  • Preparation method of protective liquid containing feline panleukopenia yolk antibodies
  • Preparation method of protective liquid containing feline panleukopenia yolk antibodies

Examples

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Effect test

Embodiment 1

[0024] A method for preparing a protective solution containing feline panleukopenia yolk antibody, characterized in that: the protective solution includes components and their weight / number ratios: 20 g of konjac gum, 20 g of purslane flavonoids, 10 g of sucrose, Lactic acid bacteria 5g, phosphate buffer 800g.

[0025] A method for preparing a protective solution containing feline panleukopenia yolk antibody, characterized in that: the steps of the protective solution preparation method are as follows:

[0026] 1. Preparation of protective solution

[0027] (1) Weigh konjac gum, purslane flavonoids and sucrose according to weight, add phosphate buffer, stir to dissolve, and then sterilize at 105°C for 10 minutes at high temperature and high humidity to obtain solution A;

[0028] (2) Formulate the lactic acid bacteria into bacterial solution B according to the weight ratio, and store it at 2-8°C for later use;

[0029] (3) Mix the solution A with the bacterial solution evenl...

Embodiment 2

[0037] A method for preparing a protective solution containing feline panleukopenia yolk antibody, characterized in that: the protective solution includes components and their weight / number ratios: 40g of konjac gum, 30g of purslane flavonoids, 30g of sucrose, Lactic acid bacteria 10g, phosphate buffer 1000g.

[0038] A method for preparing a protective solution containing feline panleukopenia yolk antibody, characterized in that: the steps of the protective solution preparation method are as follows:

[0039] 1. Preparation of protective solution

[0040] (1) Weigh konjac gum, purslane flavonoids and sucrose respectively according to weight, add phosphate buffer, stir to dissolve, and then sterilize at 120°C for 10 minutes at high temperature and high humidity to obtain solution A;

[0041] (2) Formulate the lactic acid bacteria into bacterial solution B according to the weight ratio, and store it at 2-8°C for later use;

[0042] (3) Mix the solution A with the bacterial so...

Embodiment 3

[0050] Preparation of Egg Yolk Antibody in Feline Panleukopenia

[0051] 1. Production of high-immune eggs: Select healthy unimmunized laying hens, and use pan-leukopenia vaccine to immunize chickens before laying eggs, including basic immunization, booster immunization, and booster immunization. The immunization interval is 8 days. The dosage is 1ml / bird / time, and the hyperimmune eggs are collected when the serum antibody titer reaches 1:1024;

[0052] 2. Wash and disinfect the high-free eggs, collect and stir to obtain a homogenous egg yolk;

[0053]3. Add 1.5 times the volume of normal saline and 0.1% n-octanoic acid to the egg yolk homogenate obtained in step 2, stir for 1.5 hours while adding, leave at room temperature for 6 hours, centrifuge at 5500r / min for 30min, discard Precipitate, add 1.2% dextran sulfate to the supernatant, stir for 20 minutes, filter the supernatant with a cartridge filter element with a pore size of 5 μm and 1 μm, and then concentrate 4 times th...

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Abstract

The invention provides a preparation method of a protective liquid containing feline panleukopenia yolk antibodies. The effective components and weight ratio of the protective liquid are respectivelyas follows: 20-40 parts of konjac gum; 20-30 parts of portulaca flavone; 10-30 parts of sucrose, 5-10 parts of Lactobacillus and 800-1000 parts of phosphoric acid buffer. According to the present invention, the excretion of toxins and garbage in cat body can be promoted by the natural plant effective ingredients and Lactobacillus in the protective liquid, and at the same time, the protective liquid has the effects of calming, reducing dryness, enhancing resistance, and avoiding the stress reaction of antibody immunity.

Description

technical field [0001] The invention belongs to the technical field of pet biological products, and in particular relates to a method for preparing cat panleukopenia egg yolk antibody protection solution. Background technique [0002] Feline panleukopenia is an acute highly contagious disease of cats. The incubation period is 2-9 days. The clinical symptoms are related to age and virus virulence. Most kittens are acutely ill, with body temperature rising above 40°C and severe vomiting and sudden death. Cats over 6 months old mostly show subacute clinical symptoms, with fever reaching about 40°C, which drops to normal temperature after 1-2 days, and rises again after 3-4 days, which is the biphasic fever type. The sick cat was depressed, anorexia, obstinate vomiting, the vomit was yellow-green, sticky secretions in the mouth, eyes, and nose, sticky stool, bloody in the later stage, severe dehydration, and anemia. [0003] Egg yolk antibody has the advantages of stable prope...

Claims

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Application Information

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IPC IPC(8): A61K39/42A61K31/715A61K36/185A61K9/08A61K47/26A61K47/36A61K47/46A61P31/20
CPCA61K9/08A61K31/715A61K36/185A61K39/42A61K47/26A61K47/36A61K47/46A61P31/20A61K2300/00
Inventor 陈庆忠安同伟李迎梅
Owner TIANJIN HLINTE BIOTECH CO LTD
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