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Refined panax quinquefolius total saponin component and method for preparing panax quinquefolius total saponins through purification

A technology of American ginseng and total saponins, which is applied in the field of traditional Chinese medicine preparation technology, can solve the problems of difficult pretreatment, poor strength, and serious crushing, and achieve the effects of safe and controllable operating parameters, simple preparation process operation, and increased active ingredient content

Inactive Publication Date: 2020-04-10
TAIZHOU GUOKEHUAWU BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the macroporous resin adsorption separation technology has disadvantages: (1) high organic residues, difficult pretreatment; (2) poor strength, severe breakage during use, and short service life; (3) wide particle size distribution, poor separation effect (4) For the same type of resin produced by the same manufacturer, the specific surface area and functional group content vary greatly between batches, and the reproducibility in the purification of traditional Chinese medicine components is poor, etc.

Method used

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  • Refined panax quinquefolius total saponin component and method for preparing panax quinquefolius total saponins through purification
  • Refined panax quinquefolius total saponin component and method for preparing panax quinquefolius total saponins through purification
  • Refined panax quinquefolius total saponin component and method for preparing panax quinquefolius total saponins through purification

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Experimental program
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Effect test

Embodiment 1

[0031] The present invention proposes a purification preparation method for extracting total saponins of American ginseng, the specific steps are as follows:

[0032] Weigh 100g of the pretreated American ginseng medicinal material, add 1L of 65% ethanol in a water bath, heat and reflux for extraction (90°C) for 3 hours, filter through filter paper, add 0.8L of 65% ethanol to the medicinal residues, and then heat and reflux for 2 hours in a water bath (90°C). Filtrate and combine the filtrates; concentrate by rotary evaporation and spray dry to obtain dry powder of the extract. The filtrate is carried out to HPLC analysis, and chromatographic analysis result is as follows: figure 1 shown.

[0033] Connect the C18 reverse-phase chromatographic column (filler weight 300g, particle size 60μm) to the high-pressure liquid phase system, design the flow rate 60ml / min (190cm / h), use 5% ethanol equilibrium buffer to wash the chromatographic column 3BV, and extract American ginseng L...

Embodiment 2

[0036] The present invention proposes a purification and preparation method for extracting total saponins of Panax ginseng, and the specific steps are as follows:

[0037]Weigh 100g of the pretreated American ginseng medicinal material, add 2L of 70% ethanol in a water bath, heat and reflux for extraction (90°C) for 2 hours, filter with gauze, add 1L of 70% ethanol to the medicinal residues, heat and reflux for extraction in a water bath (90°C) for 2 hours, and filter with gauze , the combined filtrates were concentrated by rotary evaporation and then spray-dried to obtain dry powder of the extract.

[0038] Connect the C18 reverse-phase chromatographic column (filler weight 300g, particle size 30μm) to the high-pressure liquid phase system, the design flow rate is 60ml / min (190cm / h), use pure water equilibrium buffer to wash the chromatographic column 3BV, and the American ginseng crude extract (Use a 0.22 μm filter membrane to remove particulate insolubles) Purify the chroma...

Embodiment 3

[0041] The present invention proposes a purification preparation method for extracting total saponins of American ginseng, the specific steps are as follows:

[0042] Weigh 100g of the pretreated American ginseng medicinal material, add 1.5L of 55% ethanol in a water bath, heat and reflux for extraction (90°C) for 2 hours, filter with gauze, add 1L of 55% ethanol to the medicinal residues, and then heat and reflux for extraction in a water bath (90°C) for 1 hour. Filtrate, combine the filtrates, concentrate by rotary evaporation, and then spray dry to obtain dry extract powder.

[0043] Connect the C18 reverse-phase chromatographic column (filler weight 300g, particle size 15μm) to the high-pressure liquid phase system, design the flow rate 50ml / min (150cm / h), use 10% ethanol equilibrium buffer to wash the chromatographic column 3BV, and extract American ginseng Liquid (use a 0.22μm filter membrane to remove particulate insoluble matter) through the system pump to flow through...

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Abstract

The invention discloses a refined panax quinquefolius total saponin component and a purification preparation method for extracting panax quinquefolius total saponins, and particularly relates to the processes of alcohol extraction, clarification and filtration, reversed-phase preparative chromatography refining, gel decoloration and impurity removal, and concentration and drying. An panax quinquefolius medicinal material is used as a raw material, and an ethanol extracting solution is used as a panax quinquefolius crude extract solution. The crude extract solution is clarified through a membrane filtration technology. Refining preparation is performed through reversed phase chromatography by utilizing a high-pressure preparation system, and finally, decoloration and impurity removal are performed by adopting ion exchange gel chromatography. The invention further provides a refined panax quinquefolius total saponin component. The raw material is mainly composed of the panax quinquefolius medicinal material, and the refined panax quinquefolius total saponin component is refined by the preparation method in each embodiment. The panax quinquefolius total saponins prepared by the methodare high in content and yield. The production process has the characteristics of simple operation of the preparation process, no need of toxic and harmful organic solvents, low cost, harmless emission to the environment and the like.

Description

technical field [0001] The invention relates to a preparation process of traditional Chinese medicine, and mainly relates to a method for preparing total saponins of American ginseng by applying high-performance liquid phase preparation and ion exchange chromatography technology. Background technique [0002] American ginseng (scientific name: Panax quinquefolius) is a perennial herbaceous plant belonging to the Araliaceae genus. Pharmacological studies have shown that the saponins in American ginseng can also protect the cardiovascular system, enhance the function of the central nervous system, and improve immunity. At present, the dosage forms of American ginseng pharmaceutical preparations are liquid and solid. In order to retain the active ingredients of American ginseng, the drug is often fed in the form of fine powder in the solid dosage form, and directly added to capsules, tablets, etc.; To put it forward to the maximum, it is also necessary to ensure that the appea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258
CPCA61K36/258A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 梁鑫淼万瑛叶贤龙郭志谋钱子俊张华林
Owner TAIZHOU GUOKEHUAWU BIOMEDICAL TECH CO LTD
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