Mass spectrometry detection method of plasma or tissue concentration of seven components of compound salvia miltiorrhiza preparation

A compound salvia miltiorrhiza and detection method technology, applied in the direction of measuring devices, material separation, and analysis of materials, can solve problems such as difficult handling and detection, large content differences, and complicated processes, and achieve good practical significance, high separation, and Simple operation effect

Active Publication Date: 2022-07-01
GUANGZHOU PHARMACEUTICAL INDUSTRIAL RESEARCH INSTITUTE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large difference in properties of the seven components of the compound Danshen preparation, water-soluble components (salvianolic acid B, danshensu, notoginsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rb 1 ) and fat-soluble components (tanshinone I and tanshinone IIA), and the content is quite different, so it is difficult to use a pretreatment method to process and detect
As a result, the current research on the detection method of the main components of the compound Danshen preparation usually adopts the method of pretreatment and detection of different components separately, the process is complicated and the steps are cumbersome

Method used

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  • Mass spectrometry detection method of plasma or tissue concentration of seven components of compound salvia miltiorrhiza preparation
  • Mass spectrometry detection method of plasma or tissue concentration of seven components of compound salvia miltiorrhiza preparation
  • Mass spectrometry detection method of plasma or tissue concentration of seven components of compound salvia miltiorrhiza preparation

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preparation example Construction

[0032] (1) Preparation of stock solution:

[0033] Take standard products Tanshinone I, Tanshinone IIA, Salvianolic acid B, Danshensu, Panax notoginseng R 1 , Ginsenoside Rg 1 and Rb 1 , respectively dissolved in (80±5)% methanol aqueous solution to prepare standard stock solution; mix the standard stock solution to obtain mixed standard stock solution;

[0034] Dissolve carbamazepine in (80±5)% methanol aqueous solution to prepare carbamazepine stock solution;

[0035] (2) Preparation of working solution:

[0036] Dilute the mixed standard stock solution with (80±5)% methanol aqueous solution respectively to prepare a mixed standard curve sample working solution and a mixed quality control sample working solution;

[0037] Dilute the carbamazepine stock solution with (80±5)% methanol aqueous solution to prepare an internal standard working solution;

[0038] (3) Preparation of sample solution to be tested:

[0039] Preparation of the plasma sample solution to be tested:...

Embodiment 1

[0092] (1) Preparation of solution

[0093] Preparation of stock solutions:

[0094] Weigh an appropriate amount of the main components of compound Danshen preparation, Tanshinone I, Tanshinone IIA, Salvianolic acid B, Danshensu, Panax notoginseng saponin R 1 , Ginsenoside Rg 1 , Ginsenoside Rb 1 The standard products were respectively dissolved in an appropriate amount of 80% methanol aqueous solution to prepare standard product stock solutions with a concentration of 1.00 mg / mL. Mix the seven standard stock solutions with a concentration of 1.00 mg / mL in equal volumes, and add an appropriate amount of 80% methanol aqueous solution to prepare a mixed standard stock solution with a concentration of 0.100 mg / mL for the seven components.

[0095] Take an appropriate amount of carbamazepine standard and dissolve it in 80% methanol aqueous solution to prepare a carbamazepine stock solution with a concentration of 1.00 mg / mL.

[0096] Preparation of standard curve working solut...

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Abstract

The invention relates to a mass spectrometry detection method for plasma or tissue concentrations of seven components of a compound salvia miltiorrhiza preparation. The method includes the following steps: (1) Preparation of stock solution: take standard products Tanshinone I, Tanshinone IIA, salvianolic acid B, Danshensu, and Panax notoginseng saponins R 1 , Ginsenoside Rg 1 and Rb 1 , were dissolved in (80±5)% methanol aqueous solution to prepare standard stock solution; carbamazepine was dissolved in (80±5)% methanol aqueous solution to prepare carbamazepine stock solution; (2) working solution (3) preparation of the sample solution to be tested; (4) separation of the sample solution to be tested obtained in step (3) by liquid chromatography; and mass spectrometry analysis of the sample separated by liquid chromatography. In the detection process of the method of the invention, the chromatographic peak shape is excellent, and the channel of each compound has only a unique target peak, which avoids other interferences such as solvent effects, and has the advantages of simple operation, rapid analysis, high specificity and high resolution.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a mass spectrometry detection method for the plasma or tissue concentrations of seven components of a compound Danshen preparation. Background technique [0002] Compound Danshen preparation is composed of three traditional Chinese medicines: Danshen, Panax notoginseng and borneol. There are 9 kinds of dosage forms: Compound Danshen Oral Liquid, Compound Danshen Aerosol, Compound Danshen Buccal Tablets, Compound Danshen Capsules, Compound Danshen Tablets, Compound Danshen Granules, Compound Danshen Dropping pills, compound salvia spray, compound salvia soft capsules. Compound Danshen preparation has the effects of promoting blood circulation and removing blood stasis, regulating qi and relieving pain. It is used for chest pain caused by stagnation of qi and blood stasis. In clinical application, the incidence of adverse reactions with compound Danshen preparation alone is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06
Inventor 殷玮孙晶晶凌珊周兰华胡钟芳谢瑞吴家欣戚园梅张尧君彭佳裕蔡文镇
Owner GUANGZHOU PHARMACEUTICAL INDUSTRIAL RESEARCH INSTITUTE
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