Fluidic HPV genotyping chip detection method

Abstract

The invention relates to the field of in vitro diagnostic reagent detection, more specifically, relates to a fluidic HPV genotyping chip detection method comprising the following steps: dropwise adding a PCR product of a sample to be detected into PCR to-be-detected liquid small test tubes of an instrument, placing an HPV genotyping chip in an HPV chip hybridization tank, preparing reaction liquidrequired by hybridization, coupling and color development and distilled water required by cleaning, and carrying out automatic detection. The beneficial effects of the invention are that: a biochemical reaction model in a flow control mode is adopted; under the cooperation of a pipetting device for vertical sample adding and a circulating pump, the processes of multi-step hybridization, coupling,color development and cleaning required by HPV detection can share one set of pipetting device, storage pool, fluid pipeline, circulating pump and biochemical reaction tank, so that the continuity ofbiochemical detection steps is ensured, and automatic detection is easier to realize.

Description

technical field [0001] The invention relates to the field of detection of in vitro diagnostic reagents, in particular to a fluidic HPV genotyping chip detection method. Background technique [0002] At present, the main methods for domestic clinical detection of human papillomavirus (Human papillomavirus, HPV) DNA are real-time fluorescent PCR and hybrid capture method. Detect the result of hybridization between the amplified product and the coated type-specific probe membrane, and then use the alkaline phosphatase system for qualitative detection, so that 21 HPV genotypes (6, 11, 16, 18, 31, 33, 35, 39 , 42, 43, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68 and CP8304) for typing detection and analysis, which has important clinical application value for preventing cervical cancer. [0003] At present, for the HPV genotyping chip of the PCR-reverse dot hybridization method, the method of sample extraction-PCR amplification-constant temperature hybridization-visible light interpret...

AI Technical Summary

Problems solved by technology

[0003] At present, for the HPV genotyping chip of PCR-reverse dot hybridization method, the method of sample extraction-PCR amplification-constant temperature hybridization-visible light interpretation is usually used, and the workload of manual intervention is relatively large.

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