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An endophytic fungus that can promote casuarina phosphorus uptake under low phosphorus environment

An endophytic fungus and casuarina technology, applied in the field of microorganisms, can solve problems such as imbalance of soil nutrient ratio, destruction of soil physical and chemical properties and microbial systems, and difficult plant absorption and utilization, and achieve the effect of improving absorption capacity.

Active Publication Date: 2021-06-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Under natural conditions, phosphorus exists in the form of inorganic phosphorus, which is difficult to be directly absorbed and utilized by plants. The soil in the south is one of the soils with the lowest phosphorus content. The seasonal utilization rate of plants for soil phosphorus is low, and the application of chemical phosphorus fertilizers is likely to cause soil pollution. Nutrient ratio imbalance, damage to soil physical and chemical properties and microbial system, is not conducive to sustainable development of soil

Method used

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  • An endophytic fungus that can promote casuarina phosphorus uptake under low phosphorus environment
  • An endophytic fungus that can promote casuarina phosphorus uptake under low phosphorus environment
  • An endophytic fungus that can promote casuarina phosphorus uptake under low phosphorus environment

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Experimental program
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Effect test

Embodiment 1

[0012] Example 1 Isolation of endophytic fungi from casuarina

[0013] 1. Main equipment

[0014] Ultra-clean workbench SW-CJ-1FD, constant temperature incubator HH•B11-II, constant temperature culture oscillator zhwy-211B, ten-thousandth balance AR1140, automatic vertical sterilizer LMQ.C-4060, ultrapure water Machine P60-CW etc.

[0015] 2. Main Reagents and Media

[0016] Reagents: 15% sodium hypochlorite, 75% ethanol, primer PAGE 11-59bp OD 1-2, DNA electrophoresis loading buffer, GoodViewTM nucleic acid dye, 2×Tap PCR MasterMix, fungal DNA extraction kit, DNA purification and recovery kit.

[0017] Medium: (1) Modified Martin agar medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0 g, magnesium sulfate (MgSO 4 •7H 2 O) 0.5g, agar 15.0g, ultrapure water 1000ml, pH 6.2~6.6.

[0018] (2) Improved Martin's liquid medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o ...

Embodiment 2

[0039] Bacterial solution preparation: The screened endophytic fungi (Z5) were cultured in potato dextrose agar medium at 28°C for one week, then inserted into 60 mL potato dextrose liquid medium, and cultured in a constant temperature shaker for 72 h (28°C, 160 r min -1 ), calculate the number of spores by hemocytometer, and dilute the cultured bacterial solution to 5.5×10 by ten-fold dilution method with sterile water 6 pcs L -1 . After that, it was diluted with 40 mL of sterile water, and this concentration and dose (100 mL) were used for inoculation in subsequent experiments.

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Abstract

The invention discloses an endophytic fungus capable of improving casuarina phosphorus absorption in a low-phosphorus environment, and the endophytic fungus Z5 belongs to the genus Botrytis ( Botryosphaeria sp. ), which was registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on November 20, 2019, with the preservation number CGMCC No.18813. The strain is isolated and purified from casuarina twigs, which can significantly increase the phosphorus uptake of casuarina, thereby promoting the growth of plants in a low-phosphorus environment.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic fungus capable of improving casuarina phosphorus absorption in a low-phosphorus environment. Background technique [0002] As one of the three essential elements for plant growth and development, phosphorus deficiency will affect various life characteristics of plants and restrict plant growth and development. Under natural conditions, phosphorus exists in the form of inorganic phosphorus, which is difficult to be directly absorbed and utilized by plants. The soil in the south is one of the soils with the lowest phosphorus content. The seasonal utilization rate of plants for soil phosphorus is low, and the application of chemical phosphorus fertilizers is easy to cause soil pollution. The imbalance of nutrient ratio will damage the soil physical and chemical properties and microbial system, which is not conducive to the sustainable development of soil. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00A01G22/00A01G24/10C12R1/645
CPCA01G22/00A01G24/10C12N1/14C12N1/145C12R2001/645
Inventor 梁安洁李键吴承祯洪滔林勇明
Owner FUJIAN AGRI & FORESTRY UNIV
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