Method for extracting ganoderma triterpenes by continuous phase transition and ganoderma triterpenes

A Ganoderma lucidum triterpenes and extraction technology, which is applied in solid solvent extraction, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of destroying heat-sensitive active substances of Ganoderma lucidum, low extraction rate, and small injection volume, etc., and achieve reduction Loss of low boiling point components, improved extraction rate, fast action effect

Pending Publication Date: 2020-02-28
SOUTH CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

Traditional heat extraction and Soxhlet extraction have low extraction rate and long time, and higher temperature will destroy heat-sensitive active substances in Ganoderma lucidum, etc.
Ultrasonic extraction, microwave extraction and supercritical extraction have small processing capacity, high cost, complicated process, and high industrial application cost
[0005] Most of the evaluation methods disclosed in the existing documents or patents on the extraction process of triterpenoids are analyzed by HPLC, and the standard substance position of known molecular weight is used to judge whether the extract exists or to calibrate the distribution interval of the molecular weight. This method is used in biological In the field of chemistry, it is a conventional method, which is rarely used in the extraction process of traditional Chinese medicine due to its complexity, and because the method uses high performance liquid phase method, the injection volume is relatively small, resulting in relatively low detection sensitivity
The evaluation method of "single lower limit" for the quantification of individual "active ingredients (or index ingredients)" that is currently widely used completely draws on the research ideas of natural medicines and is not suitable for the evaluation of the extraction process of traditional Chinese medicines.
[0006] Patent No. CN1560072A discloses a method for preparing Ganoderma triterpenes and Ganoderma polysaccharides

Method used

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  • Method for extracting ganoderma triterpenes by continuous phase transition and ganoderma triterpenes
  • Method for extracting ganoderma triterpenes by continuous phase transition and ganoderma triterpenes
  • Method for extracting ganoderma triterpenes by continuous phase transition and ganoderma triterpenes

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Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Ganoderma lucidum triterpenes continuous phase change extraction process research experiment

[0038] 1. Single factor experiment.

[0039] (1) Method

[0040] According to the characteristics of ganoderma lucidum raw materials, the present invention continuously analyzes and determines and adjusts the technological conditions. Follow the steps below:

[0041] 1) Continuous phase-change phase-change extraction of Ganoderma lucidum polysaccharides: dry the dried Ganoderma lucidum in a block at 55°C, control the moisture content to ≤15%, crush it to an appropriate mesh size, and obtain Ganoderma lucidum powder; add the Ganoderma lucidum powder to 20L In the extraction kettle, use the extractant as pure water, extract for 60 min at the extraction temperature of 60°C, the extraction pressure of 0.20MPa, and the extraction flow rate of 100L / h. Analysis is carried out under the analysis conditions to obtain Ganoderma lucidum polysaccharide;

[0042] 2) Continuou...

Embodiment 2

[0060] Embodiment 2 A kind of method of continuous phase change extraction ganoderma triterpenes

[0061] (1) Ganoderma lucidum is sliced ​​and crushed and then dried until the water content is ≤15% to obtain Ganoderma lucidum powder, and the polysaccharides are extracted through continuous phase change phase change to obtain the ganoderma lucidum dregs, and dried to control the moisture content in the ganoderma lucidum dregs to be less than or equal to 25%;

[0062] Among them, the extraction conditions of continuous phase-change phase-change extraction of polysaccharides are: the extraction agent is pure water, the extraction temperature is 60 ° C, the extraction pressure is 0.20 MPa, and the extraction flow rate is 100 L / h. Analysis is carried out under the analysis conditions of 65°C and analysis pressure of 0.10 MPa, and the extraction rate of Ganoderma lucidum polysaccharide is 35%-41.20%;

[0063] (2) Add 2000 g of the above-mentioned Ganoderma lucidum slag into a 20L e...

Embodiment 3

[0065] Embodiment 3 A kind of method of continuous phase change extraction ganoderma triterpenes

[0066] (1) Ganoderma lucidum is sliced ​​and pulverized and then dried until the water content is ≤15% to obtain Ganoderma lucidum powder. According to the same conditions as in Example 2, after the polysaccharide is extracted by continuous phase change phase change, the ganoderma lucidum residue is obtained, dried, and controlled Moisture content in ganoderma residue ≤ 25%;

[0067] (2) Add 2000 g of the above-mentioned Ganoderma lucidum slag into a 20L extraction kettle, use ethanol aqueous solution as the extractant of Ganoderma lucidum triterpenoids, set the ethanol aqueous solution concentration as 75%, extraction temperature at 60°C, pressure at 0.10MPa, and extraction flow rate at 40L / h, Pour ethanol aqueous solution into the extraction kettle, and extract continuously for 180 minutes; control the solid-liquid ratio of Ganoderma lucidum residue and extractant to 1:30g / mL; ...

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Abstract

The invention discloses a method for extracting ganoderma triterpenes by continuous phase transition and the ganoderma triterpenes. The method for extracting the ganoderma triterpenes by continuous phase transition includes the steps: slicing, crushing and then drying blocky lucid ganoderma until water content is lower than or equal to 15% to obtain lucid ganoderma powder, and extracting polysaccharides by continuous phase transition to obtain lucid ganoderma residues; taking ethanol water solution as an extraction agent after drying, enabling the extraction agent to flow through an extractionkettle at the flow speed of 20-100L/h under the conditions of the extraction temperature of 60-90 DEG C, the pressure of 0.02-0.25MPa and the ethanol concentration of 50%-95%, and performing continuous extraction for 120-300 minutes. Analysis temperature is 50-75 DEG C, and analysis pressure ranges from -0.10MPa to 0.10MPa. Compared with a traditional Soxhlet extraction method, the method has theadvantages that the extraction rate of the ganoderma triterpenes is increased by 10%-30%, extraction time is shortened by 40%-60%, the method is high in extraction rate, strong in operability, environmentally friendly and suitable for industrial production, and energy is saved.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation. More specifically, it relates to a method for continuous phase-change extraction of ganoderma triterpenes and ganoderma triterpenes. Background technique [0002] Ganoderma lucidum is a kind of large fungus, which belongs to Basidiomycetes, Polyporaceae, Polyporaceae, and Ganoderma genus in fungal classification. There are many active components in Ganoderma lucidum and their composition is complex. Currently, more than 150 compounds have been isolated, mainly including Polysaccharides, triterpenoids, sterols, alkaloids, furan derivatives, aminopeptides, and inorganic elements. Among them, Ganoderma lucidum triterpenoids are one of the important chemical components of Ganoderma lucidum, which have anti-tumor, immune regulation, hypoglycemic, anti-aging, anti-oxidation and scavenging free radicals effects. The biggest feature of Ganoderma lucidum triterpenes is that it can acti...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P35/00A61P37/02A61P3/10A61P39/06B01D11/02
CPCA61K36/074A61K2236/333A61K2236/37A61P3/10A61P35/00A61P37/02A61P39/06B01D11/02
Inventor 曹庸张军段杉何宝文戴伟杰张杏思
Owner SOUTH CHINA AGRI UNIV
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