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A kind of detection method of embedded lycopene

A technology of lycopene and a detection method, which is applied to the detection field of lycopene, can solve the problems of not being applicable to all of them, and achieves the effects of short peak time, high safety and fast analysis speed.

Active Publication Date: 2022-07-26
NANJING ZHONGKE PHARMA +1
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Problems solved by technology

[0005] At present, the pretreatment sample preparation in GB 22249-2008 (determination of lycopene in health food) standard includes the preparation of general samples and the preparation of microencapsulated samples, but now on the market for a variety of different materials embedded Lycopene, such as Israel's LycoBeads 20% VBAF (ingredients: sodium alginate, gum arabic, lycopene from red tomatoes, pea starch and water), Germany's BASF's LycoVit 10% DC (ingredients: gelatin, Sucrose, corn starch, lycopene, ascorbyl palmitate, dl-α-tocopherol, sodium ascorbate, tricalcium phosphate) and 10% lycopene microcapsules of Chenguang Biotechnology (ingredients: lycopene, bovine bone gelatin , T-66, pure water, others), these two sample preparation methods in the national standard are not applicable to all

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  • A kind of detection method of embedded lycopene
  • A kind of detection method of embedded lycopene
  • A kind of detection method of embedded lycopene

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Embodiment 1

[0043] A method for detecting embedded lycopene, the steps are as follows:

[0044] 1. Pre-treatment of the sample to be tested:

[0045] Weigh 2g of the sample to be tested and grind it thoroughly. After mixing evenly, accurately weigh 100mg and place it in a 100mL brown volumetric flask. Add 20mL of 4% ammonia buffer solution and sonicate for 20min in a 55°C water bath. Shake by hand every 5min; After room temperature, dilute to volume with tetrahydrofuran solution containing antioxidants, stir on a magnetic stirrer for 20 min, and then let stand for 5 min. After the solution is clarified, draw 1.0 mL of supernatant and place it in a 10 mL brown volumetric flask; the final volume ratio is 90: The mobile phase solution of 10% dichloromethane and methanol solution was adjusted to the scale line to obtain the solution to be tested.

[0046] The preparation method of the 4% ammonia buffer solution is as follows: firstly, 143 mL of ammonia water is added to 1 L of purified water...

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Abstract

The invention discloses a method for detecting embedded lycopene. The method comprises the steps of first pre-processing a sample to be tested to obtain a liquid to be tested, and then detecting lycopene in the liquid to be tested by high performance liquid chromatography. In the pretreatment process, 4% ammonia buffer solution was used to break and embed the sample to be tested; the mobile phase collected by high performance liquid chromatography was dichloromethane and methanol with a volume ratio of 90:10. The invention adopts high performance liquid chromatography to detect lycopene and its content, uses ammonia buffer solution to break and embed in the process of sample pretreatment, and is suitable for the content determination of various embedded lycopene. Antioxidants are added to the lycopene-dissolving solution, which delays the breakdown of lycopene. The method of the invention is fast, accurate, has good precision and repeatability, has a wide application range, and is suitable for the detection of large batches of samples.

Description

technical field [0001] The invention relates to the field of food detection, and more particularly, to a detection method of embedded lycopene. Background technique [0002] Lycopene is a kind of carotenoid, mainly distributed in tomato, watermelon, red grapefruit and other fruits, among which tomato has the highest content. Lycopene is a natural pigment that is widely found in fruits and vegetables. It has the reputation of "plant gold" and is recognized as a type A nutrient by the World Health Organization and the United Nations Food and Agriculture Organization. Lycopene is one of the strongest antioxidants found in nature. It has the ability to efficiently quench singlet oxygen and scavenge free radicals. important physiological functions. Since the human body cannot synthesize, tomatoes and their products are the main source of lycopene obtained by the human body through diet. Lycopene is usually made into liquid, tablet, soft capsule and microcapsule as dietary suppl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74G01N30/54
CPCG01N30/02G01N30/06G01N30/74G01N30/54G01N2030/027G01N2030/062
Inventor 王颖钱一帆胡浪陈素玲
Owner NANJING ZHONGKE PHARMA
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