Preparation method for biomass decomposing bacteria

Abstract

The invention discloses a preparation method for biomass decomposing bacteria. The preparation method comprises the following steps: slant culture medium preparation and strain culturing, fermentationculture medium preparation and culturing, and fermentation liquor preparation and decomposing bacteria preparation. In the step of slant culture medium preparation and strain culturing, a slant culture medium is prepared from 1.0% of glucose, 0.5% of sodium chloride, 0.7% of peptone, 0.2% of dipotassium phosphate, 20.03% of magnesium sulfate heptahydrate, 1.5% of agar powder, 1.5% of maltose, 0.4% of a yeast extract, andthe balance being water; and in the step of fermentation culture medium preparation and culturing, a fermentation culture medium is prepared from 5.0% of bran, 0.25% of urea,0.5% of sodium chloride, 0.2% of dipotassium phosphate, 1.0% of monopotassium phosphate, 0.03% of vitamin B1, andthe balance being water. According to the invention, a strain has the advantages of high alkali resistance, rapid propagation and high enzyme yield; microorganisms grow well; strains are not prone to degeneration; generated enzymes can directly act on the surfaces of various biomasses to destroy waxiness and lignin on the surfaces of the biomasses, so the lignin of the biomasses is rapidly dissociated under the interference of external charges and chemical raw materials; earlier-stage conditions are created for fiber separation, mechanical treatment and chemical treatment; and energy conservation and consumption reduction are truly achieved.

Description

technical field [0001] The invention relates to the technical field of biomass decomposition, in particular to a preparation method of biomass decomposition bacteria. Background technique [0002] With the rapid development of China's agriculture and rural areas, agricultural output straws have lost their functions of cooking fires and raising livestock, and a large number of them are abandoned in the fields. Excessive straw returning to the fields has caused crops to lack seedlings and ridges, and outbreaks of diseases and insect pests have occurred year after year. . The mass burning of straw by ordinary people in various places not only causes environmental pollution and endangers public health, but also poses a threat to the traffic safety of road and aviation, and wastes a lot of resources. Therefore, the state has issued dozens of straw industry policies to subsidize the comprehensive utilization of straw off the field. Therefore, it will be promising to use crop str...

AI Technical Summary

Problems solved by technology

However, the traditional fiber separation technology is generally high-temperature and high-pressure cooking, which has heavy pollution, low yield, large amount of chemical alkali, water and labor. The existing technology has the disadvantages of high energy consumption, high cost and serious pollution.

[0003] At present, the preparation of microbial compound bacterial agents is still limited to the production of microbial fertilizers, etc., and there is no special strain and method for decomposing crop straw for straw fiber separation.