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Method for recovering and utilizing metal resources by using Bacillus extracellular polymer

A technology of bacillus and heavy metals, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems that cannot be realized, and achieve the reduction of harm, increase of production efficiency, and simple extraction method Effect

Active Publication Date: 2021-06-22
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of these methods can realize resource treatment of wastewater, waste and soil containing heavy metals and rare and precious metals alone or

Method used

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  • Method for recovering and utilizing metal resources by using Bacillus extracellular polymer
  • Method for recovering and utilizing metal resources by using Bacillus extracellular polymer
  • Method for recovering and utilizing metal resources by using Bacillus extracellular polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Bacillus megaterium was used as the bacillus in this embodiment.

[0044]The EPS of Bacillus megaterium is prepared by the following steps:

[0045] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7-8, place it at a temperature of 100-121°C for 30-40 minutes and then prepare it as a Luria-Bertani liquid fermentation medium, using solid Luria-Bertani Culture medium, after activating and culturing Bacillus megaterium for 24-36 hours, inoculate it into Luria-Bertani liquid fermentation medium, and culture it at 23-25°C for 24-36 hours to obtain a microbial fermentation broth made of Bacillus megaterium;

[0046] In the aforementioned steps, the choice of pH value and fermentation temperature provides the best environment for the fermentation of Bacillus megaterium to produce a large amount of beneficial ingredients.

[0047] (2) Centrifuge the microbial fermentation liquid in step (1) at 5-7°C and 3000-8000r / min, let it stand after centrifugation, take the s...

Embodiment 2

[0069] In this example, Bacillus subtilis was used as the microorganism belonging to the genus Bacillus.

[0070] The EPS of Bacillus subtilis is prepared by the following steps:

[0071] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7-8, place it at a temperature of 100-121°C for 30-40 minutes and then prepare it as a Luria-Bertani liquid fermentation medium, using solid Luria-Bertani Culture medium, after activating and culturing Bacillus subtilis for 26 hours, inoculate it into Luria-Bertani liquid fermentation medium, and culture it at 23-25°C for 24-36 hours to obtain a microbial fermentation broth made of Bacillus subtilis;

[0072] In the aforementioned steps, the choice of pH value and fermentation temperature provides the best environment for the fermentation of Bacillus subtilis to produce a large amount of beneficial ingredients.

[0073] (2) Centrifuge the microbial fermentation liquid in step (1) at 5-7°C and 3000-8000r / min, let it stand after cen...

Embodiment 3

[0095] In this example, Bacillus genus microorganisms adopt Bacillus cereus

[0096] The EPS of Bacillus cereus is prepared by the following steps:

[0097] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7-8, place it at a temperature of 100-121°C for 30-40 minutes and then prepare it as a Luria-Bertani liquid fermentation medium, using solid Luria-Bertani Culture medium, after activating and culturing Bacillus cereus for 24-48 hours, inoculate it into Luria-Bertani liquid fermentation medium, and culture it at 23-25°C for 24-36 hours to obtain a microbial fermentation broth made of Bacillus cereus;

[0098] In the aforementioned steps, the choice of pH value and fermentation temperature provides the best environment for the fermentation of Bacillus cereus to produce a large amount of beneficial ingredients.

[0099] (2) Centrifuge the microbial fermentation liquid in step (1) at 5-7°C and 3000-8000r / min, let it stand after centrifugation, take the supernatant,...

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Abstract

The invention discloses a method for recovering and utilizing metal resources by utilizing the extracellular polymer of bacillus, and relates to the technical field of resource utilization and recovery. The present invention includes the extraction of microorganisms belonging to the genus Bacillus, which is extracted through the following steps: S1: After the bacillus is activated and cultivated by using a solid medium, it is inoculated into a liquid fermentation medium with a pH value of 7-8, and placed in an environment of 23-25°C fermentation and culture to obtain microbial fermentation broth; S2: place the microbial fermentation broth in an environment of 5-7°C for centrifugation, take the supernatant and filter, and freeze-dry the filtrate to obtain EPS powder; the EPS powder includes s- EPS powder, LB-EPS powder, TB-EPS powder; the present invention utilizes the aforementioned Bacillus microbial extracellular polymer to recycle and / or degrade and / or transform heavy metal ions and rare precious metals in wastewater and / or soil; In order to realize the purpose of developing an eco-friendly method for resource processing and recycling of heavy metals and rare and precious metals.

Description

technical field [0001] The invention relates to the technical field of resource utilization and recovery, in particular to the technical field of green and pollution-free treatment and recovery of heavy metals and rare precious metals by using microorganisms. Background technique [0002] Metals are essential for the sustainable production of key components for a variety of products such as automobiles, electronics, medicine and industrial activities, and demand for metals is increasing in a growing society. However, it is facing two challenges, namely the depletion of strategic metals due to limited reserves, followed by excessive environmental burdens caused by resource development and human activities. The lack of metal resources and its impact on the economy have become the main problems restricting sustainable development, while metal pollution, especially heavy metal pollution, hastened the urgency of remediation. Against this background, in pursuit of a circular econ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F1/58C12P13/00C12P7/02C22B7/00C22B11/00C22B34/32C22B23/00C22B13/00C22B17/00C22B15/00B09C1/10C02F101/22C12R1/07
CPCB09C1/10C02F1/58C02F1/62C02F2101/22C12P7/02C12P13/001C22B7/006C22B11/04C22B13/045C22B15/0084C22B17/04C22B23/0453C22B34/32Y02P10/20
Inventor 屈庆李顺灵李蕾
Owner YUNNAN UNIV
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