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Method and system of achieving large-volume and high-resolution time pulse light film tomography imaging

A time pulse and tomographic imaging technology, applied in the field of biomedicine and optical imaging, to meet the needs of large field of view, fast organ-level imaging, and expand the effect of biomedical applications

Active Publication Date: 2020-02-07
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the shortcomings of the above-mentioned background technology, the present invention proposes a time-pulse light slice tomographic imaging method and system to realize high-resolution imaging of large-volume samples or large living organisms, so as to solve the problems of light imaging resolution and imaging depth

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  • Method and system of achieving large-volume and high-resolution time pulse light film tomography imaging
  • Method and system of achieving large-volume and high-resolution time pulse light film tomography imaging
  • Method and system of achieving large-volume and high-resolution time pulse light film tomography imaging

Examples

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Effect test

Embodiment 1

[0036] Example 1 Fluorescence labeling imaging

[0037] For fluorescent label imaging, the excitation and emission wavelengths of fluorescent labeling moieties include the visible, near-infrared, and far-infrared ranges. Taking visible light excitation as an example, the wavelength of the two laser beams is 1040 nm&1130 nm, which can be used for two-color two-photon excitation of Sulforhodamine101, Texas red, and quantum dot dyes.

[0038] Pulsed laser has the advantages of ultra-short pulse and high repetition rate. Taking femtosecond light source as an example, the laser outputs two beams of synchronous light source. The thickness of the sheet; the thickness of the time light sheet or the Z-axis resolution of imaging is determined by the time pulse width of the two beams of light pulses. Pulse light sources include attosecond, femtosecond, picosecond, and nanosecond pulse lasers. Reducing the time pulse width can achieve Higher axial resolution. The repetition frequency of t...

Embodiment 2

[0054] Example 2 Vascular Imaging

[0055] The difference between this embodiment and the first embodiment is that it adopts the certified ICG dye that can be used on the human body. Its absorption peak is at 800 nm, and it can be excited by ~1300 nm and ~2100 nm in the second near-infrared region, and the excitation light has a deeper The penetration depth of a single beam of light cannot excite fluorescence, and only a time light sheet signal is generated where the two beams of light overlap to achieve tomographic imaging of blood vessels, such as Figure 5 shown;

[0056] The sample is processed by fluorescence, phosphorescence, Raman, photothermal, and photoacoustic labeling. The two pulsed light sources alone cannot excite the labeling group to send a signal, and only when the two pulsed light sources act together can the signal be generated.

Embodiment 3

[0057] Example 3 SRS or CARS imaging of fat and protein

[0058] The difference between this embodiment and Embodiment 1 is that the sample is not fluorescently labeled, and the two light pulses λ 1 , lambda 2 Exciting endogenous molecules in the sample to emit signals when they coincide simultaneously in space and time, said signals include optical, acoustic, thermal, electrical, magnetic signals. The laser chooses pump light and Stokes light.

[0059] The specific implementation process of SRS: select the wavelength of the pump light and the Stokes light to excite specific analytical chemical bonds, modulate the Stokes light through the acousto-optic modulator, and output the reference signal of the modulator to the lock-in amplifier ; After a suitable time delay, then coincide with the pump light on the sample, and use a high-pass low-reflection dichroic mirror to reflect the pump light to the photodiode for detection on the optical path of the forward propagation of the ...

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Abstract

The invention discloses a method and system of achieving large-volume and high-resolution time pulse light film tomography imaging. The method includes the steps: 1, a laser device outputs two synchronous or phase-locked pulse light beams [lambda]1 and [lambda]2; 2, the pulse light beam[lambda]2 is matched with a time delay device to be subjected to time delay adjustment; 3, the pulse light beams[lambda]1 and [lambda]2 are completely emitted oppositely, or angularly emitted oppositely or angularly emitted in the same direction, by adjusting the positions of the pulse light beams, space overlapping and time pulse overlapping on an imaging sample are achieved synchronously, and then a time light film is formed; 4, a section where the time light film is located produces signals to provide image information; and 5, by changing relative time delay of the light beams or the position of the sample, tomography scanning imaging of the light film is achieved. According to the method and systemof achieving large-volume and high-resolution time pulse light film tomography imaging, the modulated time pulse light film is adopted, so that the problems are solved that regarding mechanical lightfilms, resolutions are not uniform, large backgrounds are defocused during depth imaging, and signal-to-background ratios are low; and therefore, the method and the system can be used for large-volumeorgan-level imaging.

Description

technical field [0001] The invention relates to the technical fields of biomedicine and optical imaging, in particular to a method and system for realizing large-volume and high-resolution time-pulse light slice three-dimensional tomographic imaging. Background technique [0002] In biomedical research and practical applications, optical imaging techniques including confocal fluorescence microscopy, multiphoton fluorescence microscopy and other imaging modalities have been widely used to observe the fine structure and functional activities of biological tissues. Although optical imaging technology has many advantages such as non-toxicity, high resolution, fast imaging speed, and high sensitivity, it is only suitable for imaging the surface layer of biological tissue from a few microns to a few millimeters in depth. For larger biological tissues or other opaque samples (1 mm to 10 cm and above), as the imaging depth increases, light scattering and absorption tend to be seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00A61B5/026
CPCA61B5/0059A61B5/0073A61B5/0261A61B2576/026
Inventor 王平杨驰毕亚丽
Owner HUAZHONG UNIV OF SCI & TECH
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