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Method for preparing bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing

A bioprinting, composite tissue technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of providing bone lesions, inability to combine, muscles without function, etc., and achieves simple preparation method, small fibrosis, and comprehensive performance. Good results

Active Publication Date: 2020-02-04
福建省安悦莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However autografts are limited by the amount of bone and may cause lesions at the donor bone site during reconstructive surgery
Although a flap involving bone and muscle can be transferred, the muscle is nonfunctional and cannot be combined with the supporting musculature

Method used

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  • Method for preparing bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing
  • Method for preparing bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing
  • Method for preparing bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A bionic skeletal muscle composite tissue prepared by multi-channel extrusion 3D bioprinting, prepared by the following preparation method:

[0067] S1. By dropping MA into the gelatin aqueous solution, the degree of substitution of MA in the bionic bone is 81.4%, that of the bionic periosteum is 19.7%, that of the bionic muscle fiber membrane is 19.7%, and that of the bionic muscle is 19.7%.

[0068] S2. The concentration of GelMA in the bionic bone is 7.0% (W / V), the concentration of SA is 0.5% (W / V) and the concentration of gelatin is 3.0% (W / V); the concentration of GelMA in the bionic periosteum scaffold is 5.0 % (W / V), the concentration of SA is 0.5% (W / V) and the concentration of gelatin is 2.0% (W / V); the concentration of GelMA in the biomimetic sarcolemma scaffold is 5.0% (W / V), the concentration of SA The concentration is 0.5% (W / V) and the concentration of gelatin is 2.0% (W / V); the concentration of GelMA in the bionic muscle scaffold is 5.0% (W / V), the conce...

Embodiment 2

[0075] A bionic skeletal muscle composite tissue prepared by multi-channel extrusion 3D bioprinting, prepared by the following preparation method:

[0076] S1. By adding MA dropwise to the gelatin aqueous solution, the substitution degree of MA in the bionic bone is 90.0%, that of the bionic periosteum is 25.0%, that of the bionic muscle fiber membrane is 25.0%, and that of the bionic muscle is 25.0%.

[0077] S2. The concentration of GelMA in the bionic bone is 6.0% (W / V), the concentration of SA is 0.5% (W / V) and the concentration of gelatin is 3.0% (W / V); the concentration of GelMA in the bionic periosteum scaffold is 4.0 % (W / V), the concentration of SA is 0.5% (W / V) and the concentration of gelatin is 2.0% (W / V); the concentration of GelMA in the biomimetic sarcolemma scaffold is 4.0% (W / V), the concentration of SA The concentration is 0.5% (W / V) and the concentration of gelatin is 2.0% (W / V); the concentration of GelMA in the bionic muscle scaffold is 4.0% (W / V), the con...

Embodiment 3

[0084] A bionic skeletal muscle composite tissue prepared by multi-channel extrusion 3D bioprinting, prepared by the following preparation method:

[0085] S1. By dropping MA into the gelatin aqueous solution, the degree of substitution of MA in the bionic bone is 81.4%, that of the bionic periosteum is 19.7%, that of the bionic muscle fiber membrane is 19.7%, and that of the bionic muscle is 19.7%.

[0086] S2. The concentration of GelMA in the bionic bone is 7.0% (W / V), the concentration of SA is 0.5% (W / V) and the concentration of gelatin is 4.0% (W / V); the concentration of GelMA in the bionic periosteum scaffold is 5.0 % (W / V), the concentration of SA is 0.5% (W / V) and the concentration of gelatin is 3.0% (W / V); the concentration of GelMA in the biomimetic sarcolemma scaffold is 5.0% (W / V), the concentration of SA The concentration is 0.5% (W / V) and the concentration of gelatin is 3.0% (W / V); the concentration of GelMA in the bionic muscle scaffold is 5.0% (W / V), the conce...

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Abstract

The invention discloses a method for preparing a bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing. The preparation method comprises the following steps: preparing bone scaffold bionic bio-ink, periosteum bionic bio-ink, sarcolemma bionic bio-ink and muscle bionic bio-ink; respectively mixing MSCs and C2C12 with the corresponding bionic bio-inks; and printing and forming a bionic bone, bionic periosteum, bionic sarcolemma and bionic muscle four-layer composite tissue engineering scaffold by using a multi-channel extrusion 3D biological printer. Themethod for preparing the bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing can minimize fibrosis during traumatic skeletal muscle injury recovery; the bionic skeletal muscle composite tissue prepared through multi-channel extrusion 3D biological printing can replace structures and functions of bones and skeletal muscles at the same time, and supports proliferation and differentiation of myoblasts and osteoblasts; and an implant is easy to customize by utilizing a 3D biological printing technology, so that the implant is suitable for any defect shape.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a bionic skeletal muscle composite tissue prepared by multi-channel extrusion 3D bioprinting. Background technique [0002] Skeletal muscle is one of the largest and most important organs in the human body, accounting for 45% of body weight (Choi, J.S. et al. Journal of Controlled Release. 2016, 222:107-115). More than one in two of these are affected by skeletal muscle injuries. Severe traumatic muscle injuries from motor vehicle accidents, crush injuries, and explosions are all causes of severe disability and lead to severe pain and lengthy recovery periods, increasing the financial burden on the patient (Yelin, E., et al . Seminars in Arthritis and Rheumatism. 46(3):259-260). Muscle function depends on proper insertion points on stable bone, so wounds where both bone and muscle are damaged heal particularly poorly. Although recently in bone — Progress has been made in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/22A61L27/20A61L27/12A61L27/38A61L27/50B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/12A61L27/20A61L27/222A61L27/3821A61L27/3826A61L27/3873A61L27/50A61L2430/30B33Y10/00B33Y70/00B33Y80/00C08L5/04
Inventor 张进黄恒童冬梅刘晓晨李飞翰
Owner 福建省安悦莱生物科技有限公司
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