Skin biomaterial

A biomaterial and skin technology, applied in the field of skin repair biomaterials, can solve the problems of weak ability to promote tissue regeneration, poor tissue induction ability, three-dimensional structure breakage, etc., to achieve rich dosage forms, promote repair, new blood vessels and basement membrane form the effect of promoting

Pending Publication Date: 2020-01-31
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the shortcomings and deficiencies of existing skin repair materials, due to long-term, sometimes excessive, even harsh decellularization, such as using trypsin to digest, or using chemical surfactants (decellularization) for a long time Stain agent) treatment, resulting in serious damage to the three-dimensional structure of the active ingredients, cell growth factors, and extracellular matrix (ECM) in animal tissue-derived biomaterials; thus resulting in weak tissue regeneration or promoting tissue regeneration in biomaterials (patch) Wound h

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment one: (newborn calf corium+saponin)

[0062] Choose a healthy newborn calf, take its dermis, and prepare a skin biological patch. The specific steps are as follows:

[0063] 1) Pre-treatment: take materials and clean them. Select healthy newborn bulls from the cattle farm. After taking blood to prepare serum and kill them, use a knife to take the skin in the middle of the abdomen, clean it thoroughly, remove the fat on the inner surface and the outer layer mechanically, and use it to remove Rinse it with deionized water, and take a dermis tissue with a thickness of 0.3mm (including the papillary layer, and the connected basement membrane and reticular layer) with a skin extractor; soak it in a solution of 0.5% acetic acid for 20 minutes, and the newborn calf dermis The weight ratio to the acetic acid solution is 1:8, and then rinsed with purified water for 3 times; followed by disinfection, soak the leather material at room temperature for 45 minutes under ultra...

Embodiment 2

[0066] Embodiment two: (just weaned piglet SIS+saponin)

[0067] Select healthy weaned piglets, take the small intestinal submucosa, and prepare skin biomaterials. The specific steps are as follows:

[0068] 1) Pre-treatment: take materials and clean them, select healthy three-yuan mixed piglets that have reached the weaning age and weight, take their small intestines, clean them and use them as young animal tissue raw materials; after in-depth understanding, the age of piglets at weaning is usually 21- -30 days, due to different seasons, the weaning age is different, it is shorter in summer and longer in winter; the weight is about 6 to 8 kg, and the piglets mainly eat breast milk before weaning, and the full price feed they consume (Some are called creep feed or piglet milk powder) are rare. The mucosal layer, muscular layer, serosal layer, lymph nodes, etc. of the small intestine and jejunum were removed by mechanical scraping, and cut into sections to obtain the small int...

Embodiment 3

[0071] Embodiment three: (control group one, commercial meat pig)

[0072] The specific operation in the preparation method is basically the same as that of Example 2, the main difference being that the pigs have different ages and body weights. In this example, the commercial pork pigs from the slaughterhouse were selected, with a body weight of nearly 100 kg and a number of days of feeding of 150 -about 160 days; other processing methods, steps, methods such as pretreatment (comprising pretreatment, disinfection, degreasing), decellularization, follow-up processing and reagents used, concentration and action time, are completely with embodiment two.

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PUM

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Abstract

The invention relates to a skin repair biomaterial, and is used for repairing and treating acute or chronic skin wound surfaces for healing of the skin wound surfaces. The skin repair biomaterial comprises young animal tissue without cells, such as derma of newboth calves, and SIS of weaned pigs. The biomaterial adopts mild plant source reagents such as saponin for cell removing treatment on youngand tender tissue of young animals under the condition of low temperature. The ECM stereostructure of the prepared biomaterial is complete, the content of bioactivity components is high, particularlythe content of hyaluronic acid (HA) is high, generation of skin wound surface blood vessels can be well promoted, and the chemotaxis, the growth and the proliferation of the wound surface fibroblastcells are favorable for healing of the wound surfaces. The skin repair biomaterial overcomes the defects that a conventional product is low in the number of active components, high in ECM structure destroying, low in new angiogenesis and difficult in wound healing. In addition, the product namely a preparation formulation can also be prepared into different shapes of powder and the like, is flexible to use, and is simple, convenient and painless.

Description

[0001] Technical field: the present invention belongs to the field of biological repair materials, in particular to a skin repair biological material. Background technique: [0002] The skin is the largest organ of the human body and an important barrier between the human body and the external environment. It can prevent excessive loss of water in the body, and also has functions such as temperature regulation, sensation, heat insulation, and immunology. Skin is composed of epidermis, dermis and subcutaneous tissue. Once the skin is traumatized, the larger the wound area, the greater the possibility of infection. Traumatic experiences such as burns and ulcers lead to loss of skin integrity and can lead to severe disability. Studies have shown that wound repair involves the coordination and integration of a series of complex physiological and biological processes, including cell migration, proliferation, extracellular matrix deposition and remodeling, and is a complex multicel...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/60
CPCA61L27/3604A61L27/362A61L27/3629A61L27/3687A61L27/60
Inventor 韩韦红葛翠兰钱锵张国强
Owner 上海白衣缘生物工程有限公司
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