A kind of compound Bacillus inoculum and preparation method and application
A technology of compounding bacillus and bacillus, applied in the field of microorganisms, can solve the problems of reduced efficacy, weakened efficacy, and reduction of beneficial bacteria in the root system, and achieve the effects of stable efficacy, easy operation, and cost saving
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] A preparation method of a compound Bacillus microbial inoculum is carried out by the following steps:
[0030] (1) The preserved Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 were inoculated into liquid LB medium respectively, and cultured at 30℃±1℃ until In the logarithmic growth period, the concentration of seed solution is not less than 1.0×10 8 cfu / mL, to obtain seed solution for inoculation;
[0031] (2) mixing the methylotrophic Bacillus Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 obtained in step (1) to obtain a mixed seed solution, and the methyl group in the mixed seed solution The ratio of viable bacteria of Bacillus methylotrophicusKNK-1, Bacillus cereus BCJB01 and Bacillus megateriumBMJBN02 was 2:2:6;
[0032] (3) inoculate the mixed seed liquid obtained in step (2) into the fermentation medium with an inoculation amount of 10% (volume percentage), so that the initial con...
Embodiment 2
[0040] Embodiment 2 Application of compound bacillus inoculum in promoting pepper factory seedling raising and production
[0041] Proceed as follows:
[0042] (1) The compound Bacillus inoculum is added to the seedling matrix at 10 g / L, and the cultivation matrix is obtained after fully mixing, sowing and routine management of "Zhongjiao No. 6" according to the conventional method, directly using the seedling matrix (the Formula: The formula of the seedling substrate produced by Pederman Company: organic matter content ≥ 45%, humic acid content ≥ 15%, PH value: 5.5 ~ 6.8, free water content ≤ 20%) "Zhongjiao No. 6" as a cultivation substrate "The cultivation group is the negative control, and the cultivation group of "Zhongjiao No. 6" which is added with nitrogen, phosphorus and potassium compound fertilizer (nitrogen: phosphorus: potassium = 15:15:15) in the cultivation substrate is the positive control (the usage amount is 60g / m2). 2 ), 30 plants were treated in each gro...
Embodiment 3
[0051] Embodiment 3 Application of compound bacillus inoculant in preventing and controlling pepper diseases (comparison of product of the present invention and common pesticides in the field)
[0052] The application of a compound bacillus inoculant in preventing and treating pepper diseases is carried out through the following steps:
[0053] (1) The test object is the seedlings of the moderately sensitive variety "Zhongjiao No. 6" with the same growth in Example 2 (1). The test was carried out in the greenhouse experimental field of the Institute of Ecology, Shandong Academy of Sciences. The experimental field had outbreaks of chili frost in 2017 and 2018. Mildew, pepper blight and pepper blight.
[0054] (2) A total of 5 treatments were tested: the seedlings of "Zhongjiao No. 6" treated with the compound Bacillus in Example 2 (1) were composed of 45% chlorothalonil 200 times liquid, 64% antiviral alum wettable powder 500 times, the compound Bacillus inoculum is 100 times ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com