Platelet-rich plasma (PRP)-loaded pancreatic decellularized scaffold and preparation method thereof

Abstract

The invention discloses a platelet-rich plasma(PRP)-loaded pancreatic decellularized scaffold and a preparation method thereof and belongs to the technical field of pancreatic tissue engineering. PRPrich in various vascularization promoting growth factors is assembled in vessels of the pancreatic decellularized scaffold while human umbilical vein endothelial cell is planted in the vessels of thepancreatic decellularized scaffold, the loaded PRP is activated slowly by collagenous fiber of the pancreatic decellularized scaffold to releases a great quantity of growth factors slowly continuouslylocally, thereby providing an ideal environment for adhesion and proliferation of the human umbilical vein endothelial cell surrounding the inherent vascular networks in the scaffold; the PRP loadedpancreatic decellularized scaffold keeps the natural structure, biological characteristics and low immunogenicity of the pancreatic tissue; the growth factors which are slowly released continuously locally after the scaffold is transplanted in vivo have a remarkable vascularization promoting function; the scaffold is convenient to operate and has good controllability; generation of new vessels inthe scaffold is facilitated.

Description

technical field [0001] The invention belongs to the technical field of pancreatic tissue engineering, and in particular relates to a PRP-loaded pancreatic decellularized scaffold and a preparation method thereof. Background technique [0002] As a global disease, nearly 3.4 million people die from diabetes and its complications every year. The current effective way to treat type 1 diabetes is to implant islet cells. However, the long-term survival of islets is not satisfactory. The islet cells transplanted into diabetic animals do not have a good colonization environment and are easily cleared by the body, which reduces the long-term efficacy . Therefore, current research on pancreatic tissue engineering scaffolds focuses on recreating the living environment of pancreatic islets. The recently proposed pancreatic decellularized scaffold (PDS) removes cellular components, including DNA and surface antigens, but retains cell attachment points, Structural integrity and vascula...

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Problems solved by technology

[0003] At present, in order to solve the problem of vascularization of decellularized scaffolds, researchers have reconstructed the vascular network in decellularized scaffolds by coupling anti-endothelial cell antibodies (In Kap Ko et al. 2014), or constructed re-endothelialization by gelatin hydrogel modification. decellularized scaffolds (Fanwei Meng et al. 2018), but the above method is more complicated to operate, and the long-term effect is not ideal

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