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Stenotrophomonas rhizophila s11 for controlling rice blast and its application

A technology for rice blast and oligotrophic monocytogenes, which is applied in the application, bacteria, fungicides and other directions to achieve the effects of strong affinity, improved efficiency and strong antagonism

Active Publication Date: 2022-03-15
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although plant protection workers have isolated a number of biocontrol strains that can significantly inhibit the growth and development of rice blast fungus, there is still a long way to go to effectively prevent and control rice blast in the field. Biocontrol strains with control ability and easy to colonize in rice fields, finding new high-efficiency biocontrol strains will help the green control of rice blast

Method used

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  • Stenotrophomonas rhizophila s11 for controlling rice blast and its application
  • Stenotrophomonas rhizophila s11 for controlling rice blast and its application
  • Stenotrophomonas rhizophila s11 for controlling rice blast and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Screening and identification of Stenotrophomonas rhizophila S11 strain

[0039] (1) Sample collection: The samples were collected from paddy field soil in the early winter dormant season, and the location was Songyuan City, Jilin Province. Take the 5-20cm permafrost blocks on the surface, pack them in polyethylene plastic bags, and bring them back to the laboratory for separation.

[0040] (2) Isolation of bacterial strains: use 100mL Erlenmeyer bottle with 50mL sterilized water, weigh 5g soil sample (removing residual branches and broken leaves), shake it on a shaker for 30min, take it out and let it stand for 10min, absorb the supernatant, and carry out the test with sterile water. Gradient dilution, the diluted concentration has 10 -3 g / mL, 10 -4 g / mL, 10 -5 g / mL and 10 -6 g / mL, draw 100 μL to LB medium (each liter of LB medium contains: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g), spread evenly, set three replicates for each concentratio...

Embodiment 2

[0050] Determination of Inhibitory Activity of Stenotrophomonas rhizophila S11 Strain against Magnaporthe grisea

[0051] The plate confrontation culture method is used for testing, and the solid plate is a PDA medium (each liter contains: 200g potato boiled filtrate, 20g glucose, 15g agar powder, pH value is 7.0-7.2). One side of the PDA medium was inoculated with Magnaporthe grisea JJ88 (the bacterium was provided by the Plant Molecular Biology Laboratory of Jilin University), and the prepared Stenotrophomonas rhizophilus S11 strain was inserted into the opposite side by streaking , placed at 28°C for confrontation culture, and the inhibitory ability of Stenotrophomonas rhizophilus S11 strain to the growth of Magnaporthe grisea was determined, and sterile water was used as a control. Three repetitions were set for each treatment, and the growth of Magnaporthe grisea was observed and measured after 7 days.

[0052] The plate confrontation culture results showed that the colo...

Embodiment 3

[0055] Preparation of Stenotrophomonas rhizophila S11 biocontrol agent and detection of its antibacterial activity

[0056] (1) Preparation of Stenotrophomonas rhizophilus S11 biocontrol agent

[0057] Preparation of Stenotrophomonas rhizophila S11 seed liquid: The confrontation culture test in Example 2 has shown that the Stenotrophomonas rhizophila S11 strain cultured on PDA can produce compounds that inhibit the growth of Magnaporthe grisea. Therefore, the PDB liquid medium with a similar formula (each liter containing: 200g potato boiled filtrate, 20g glucose, pH 7.0-7.2) was used to ferment the S11 biocontrol strain.

[0058] Specifically: pick fresh colonies of Stenotrophomonas rhizophilus S11 (CGMCC No.18346) cultured by streaking, inoculate them into PDB liquid medium, and inoculate them at 28-30°C with a shaking rate of 180-200rpm. Cultivate for about 24 hours. After 16 hours, take samples every 3 hours in the ultra-clean workbench and measure the OD value at 600nm. ...

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Abstract

The invention discloses Stenotrophomonas rhizophila S11 for preventing and treating rice blast, which is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.18346; and discloses Stenotrophomonas rhizophila Application of Bacillus sp. S11 in the preparation of medicines for controlling rice blast. At the same time, it also discloses a biocontrol agent for preventing and treating rice blast, a preparation method thereof, and an application in preventing and treating rice blast. The biocontrol agent includes the Stenotrophomonas rhizophilus S11. In the present invention, through the measurement of plate confrontation culture method, it is found that the S11 strain has a strong antagonistic effect on the rice blast fungus, and the diameter of the inhibition zone can reach 44mm; both greenhouse inoculation and field tests show that the S11 biocontrol fungus can effectively control rice blast. Therefore, the Stenotrophomonas rhizophilus S11 strain disclosed by the present invention can be used for biological control of rice blast, and has broad promotion and application prospects.

Description

technical field [0001] The invention relates to the technical field of crop biological control, in particular to a Stenotrophomonas rhizophilus S11 for preventing and treating rice blast and its application. Background technique [0002] Rice is one of the three cereal crops with an annual output of more than 50 million tons in the world, and it is the main source of basic food for more than 3 billion people in the world (especially in Asia) every day. Rice blast disease (rice blast disease) is one of the most serious rice diseases, which occurs in all rice producing areas in the world, and can cause a 10-30% reduction in rice production every year. All tissues and parts of rice can be affected. For example, leaf blast occurs on leaves, which is the most common disease, which can lead to death of leaves in severe cases. In addition to leaves, stems can also suffer from disease, forming node blast. Lodging and withering; when the disease occurs at the heading stage, it is ea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCC12N1/20A01N63/00C12R2001/01C12N1/205
Inventor 张世宏李桂华魏毅李柱刚胡文俊李兰慧
Owner JILIN UNIV
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