Tissue hybridization in situ diagnosis and detection system for BKV and application thereof

Abstract

The invention relates to the technical field of gene diagnosis, in particular to an in-situ nucleic acid diagnosis and detection system for BKV. The in-situ nucleic acid diagnosis and detection systemcomprises probes used for detecting the BKV, sequences of the probes are the sequences or complementary chains correspondingly shown in SEQ ID NO: 1-40. The invention further discloses application ofthe in-situ nucleic acid diagnosis and detection system to preparation of diagnosis or detection reagents / kits for the BKV. The tissue hybridization in situ diagnosis and detection system for the BKVis high in sensitivity and good in specificity, and can be used for accurately diagnosing the activation of the BKV to guide clinical symptomatic medication, and the sensitivity and specificity of the BKV tissue diagnosis are improved. According to the application of the BKV hybridization in situ technology, the sensitivity and specificity of the BKV tissue diagnosis can be greatly improved, theharm caused by BKV related diseases is reduced, and wide market application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of gene diagnosis, in particular to an in-situ nucleic acid diagnosis and detection system for BKV and its application. Background technique [0002] In situ hybridization (In-Situ Hybridization, ISH) is a technology that uses nucleic acid molecular probes to quantify and locate specific nucleic acid (DNA and RNA) targets in fixed tissues and cells, and can obtain time and space related to gene expression and genetic loci Information is one of the commonly used methods of tissue diagnosis in clinic. Immunohistochemistry (IHC) is the most commonly used method in tissue diagnosis. It utilizes the principle of antigen-antibody specific binding, carries out color reaction through antibody labeling, and performs qualitative, localization, and quantitative determination of the corresponding antigen (i.e., protein). a technology. BK polyomavirus (BKpolyomavirus, BKV) is a kind of opportunistic infection virus tha...

AI Technical Summary

Problems solved by technology

Therefore, this detection technique has the following deficiencies: 1) poor specificity, which easily leads to misjudgment

2) The sensitivity is poor, which can easily lead to missed judgments

Therefore, once BKV-related nephritis is missed as BKV false negative, it will be judged as acute rejection, and the intensity of immunosuppression needs to be increased, which will lead to the aggravation of BKV-related nephritis symptoms and cause serious consequences such as transplanted kidney failure

At present, there are sporadic reports on the diagnosis of BKV in situ hybridization in the literature, but because the target site is not limited to the gene coding region, or only considers the BKV conserved region but not the specific region, it can only target DNA (a small amount) ) problem of poor sensitivity; or probes targeting BKV, JCV and SV40 at the same time and cannot distinguish the problem of poor specificity

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