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Early-stage cancer detection method based on terahertz attenuated total reflection mode

A technology of attenuated total reflection and cancer detection, which is applied in measuring devices, material analysis through optical means, instruments, etc., can solve the limitations of the direct application of terahertz diagnostic technology, high requirements for experimental conditions and operating techniques, and increase the difficulty of data post-processing and other problems, to achieve the effect of real-time rapid detection of cancer, no reagents, and simplified pretreatment process

Inactive Publication Date: 2019-12-10
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

Therefore, the transmission detection has the following problems: (1) The sample pretreatment process is complicated and delicate, and requires high experimental conditions and operating techniques, which limits the direct application of terahertz diagnostic technology in the clinical environment
(2) The test result is affected by the thickness error of the sample, which reduces the test accuracy
(3) Transmission detection will bring standing wave resonance interference in the detection results, which increases the difficulty of data post-processing

Method used

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  • Early-stage cancer detection method based on terahertz attenuated total reflection mode

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Embodiment 1

[0032] It is planned to use the Menlo Tera SYNC terahertz time-domain spectrometer to integrate with the attenuated total reflection module, and build a reference optical path such as figure 1 shown. Adjust the positions of the terahertz wave transmitting antenna and receiving antenna so that the terahertz wave is P-polarized, and adjust the transmitting antenna, attenuated total reflection module, and receiving antenna to the same horizontal line, and adjust the time delay of the terahertz time-domain spectrometer to obtain terahertz Signal. The propagation of the terahertz beam in the attenuated total reflection sampling module is as follows: the module is placed horizontally, the terahertz wave transmitter projects the terahertz beam horizontally onto a coupling surface of the prism, and the terahertz wave is refracted by the nitrogen-prism interface Entering the prism and projecting onto the sample holding pool on the total reflection surface, the terahertz wave undergoes...

Embodiment 2

[0034] A label-free method for detecting the binding reaction of aptamers and target molecules by terahertz spectroscopy. Using terahertz spectroscopy and molecular dynamics simulation, the changes in the terahertz absorption spectrum of the solution and the changes of the hydrogen bond network in the solution were studied when the tumor marker MUC1 polypeptide and its corresponding aptamer Anti-MUC1 had a specific binding reaction. The results show that the terahertz spectrum can sensitively reflect the binding reaction of MUC1 and Anti-MUC1, and the minimum detection concentration of this method is 1pmol / μL, such as figure 2 shown. Among them, (a) schematic diagram of terahertz time-domain spectrometer and dynamic range in frequency domain; (b) schematic diagram of three-dimensional model of microfluidic chip; (c) MUC1 tumor marker (red) and other observed in molecular dynamics simulation Snapshot of the aptamer (blue) binding process; (d) Concentration-dependent detection...

Embodiment 3

[0036] Combining metamaterial biosensing chips with terahertz spectroscopy detection, a label-free study of DNA oligonucleotides with 5 base mutations at the 3' end was carried out. Studies have shown that the sensitivity of terahertz metamaterial biosensor chips is sufficient to distinguish DNA oligonucleotide chains with different mutant sequences. Further, through molecular dynamics simulations, the project applicant explored the number of hydrogen bonds formed between oligonucleotides and their surrounding water molecules, which play a key role in the terahertz absorption of biological solutions, such as image 3 shown. This work laid the foundation for the research on the label-free detection of DNA by terahertz spectroscopy combined with metamaterial technology, and laid the foundation for the research of gene mutation based on terahertz spectroscopy. Among them, (a) the DNA oligonucleotide sequence involved in this study, the last 5 bases were mutated; (b) the number o...

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Abstract

The invention discloses an early-stage cancer detection method based on a terahertz attenuated total reflection mode, a physical model of interaction between multiple interfaces of a total reflectionsurface, a sample pool bottom surface, a cell layer and a buffer solution layer and evanescent waves is established, and total reflection occurs at the sample pool bottom surface-cell layer interfacebecause the total reflection surface and the sample pool bottom surface are made of the same material. The detection of the terahertz characteristic fingerprint spectrum based on the cells has highersensitivity and accuracy than the detection based on the cell morphology.

Description

technical field [0001] The invention belongs to the technical field of early cancer detection, in particular to an early cancer detection method based on a terahertz attenuated total reflection mode. Background technique [0002] Cancer is the first cause of death among urban and rural residents in my country. There are 4.29 million new cancer cases in my country every year, accounting for 20% of new cases in the world, and 2.81 million deaths. Cancer prevention and treatment has become an important public health issue in our country. A large number of studies have shown that the treatment effect on early cancer is significantly better than that of middle and advanced cancer. If the cancer can be accurately diagnosed in the early stage, it may be cured, effectively controlled or prolong the onset period of the cancer, thereby improving the patient's quality of life and length of life. Therefore, many countries, including my country, regard the early diagnosis and treatment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3581G01N33/574
CPCG01N21/3581G01N33/574G01N33/57484G01N21/552G01N21/3586
Inventor 刘钱府伟灵刘璐张明焜
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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