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A method for isolating and culturing cells from aged human synovial articular cartilage

A technology for the separation of synovial joints and cartilage, which is used in the field of separating and culturing cells from synovial articular cartilage of the elderly, and the field of primary human cell culture. It can solve the problems of low activity and low cell yield, achieve low pollution probability, and promote in vitro proliferation. , the effect of large scale

Active Publication Date: 2021-03-09
JIANGNAN UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for separating and culturing cells from the synovial articular cartilage of the elderly body, which can effectively promote the separation of chondrocytes in the synovial articular chondrocytes. Proliferation in vitro as adherent monolayer growth in culture dishes

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  • A method for isolating and culturing cells from aged human synovial articular cartilage
  • A method for isolating and culturing cells from aged human synovial articular cartilage
  • A method for isolating and culturing cells from aged human synovial articular cartilage

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Embodiment Construction

[0027] Applicants used 18 cases of femoral head or tibial plateau cartilage tissue (17 from patients with femoral neck fractures who underwent total hip replacement surgery and 1 from patients with advanced osteoarthritis who underwent total knee replacement surgery. All patients were 65 years of age over the age.

[0028] Hereinafter, a specific embodiment of the present invention will be further described by taking a femoral head specimen produced from hip arthroplasty as an example.

[0029] In the operating room, put the removed femoral head into a sterile sampling bag filled with sterile saline, and after sealing it, transport it from the operating room to the cell culture room in an ice box within four hours after the operation. In a biosafety cabinet with sterile air blowing, transfer the femoral head from the sterile sampling bag to a sterile 15 cm petri dish, and add about 10 ml of DMEM / F12.

[0030] Specific sources such as figure 1 As shown, A is the tissue of the...

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Abstract

The invention relates to a method for separating and culturing cells from synovial articular cartilage of old people, and belongs to the technical field of human tissue engineering. A large number ofcells with metabolic activity are obtained from senile articular cartilage, and the method is applied to molecular pathology and biological treatment fundamental researches for repairing cartilage tissue defects and cartilage degenerative changes through clinical autologous cartilage cell transplantation. The invention discloses a chondrocyte separation method. According to the method, mechanicalcutting and enzymatic hydrolysis of cartilage tissues are combined. Cartilage fragments are in full contact with degradable cartilage extracellular matrix protein specific protease in a tissue decomposition bottle (Spiner flasK) sterilized at high temperature and high pressure and in a cell incubator, thus achieving the purpose of completely dissolving a matrix to release cartilage cells to the maximum extent. Compared with an existing human load-bearing synovial articular cartilage cell separation method, the method has the advantages that the yield of cartilage cells is higher, the proportion of living cartilage cells is larger and the pollution probability is lower.

Description

technical field [0001] The invention relates to a primary culture technology of human cells, in particular to a method for separating and culturing cells from synovial articular cartilage of aged human body, and belongs to the technical field of human tissue engineering. Background technique [0002] The weight-bearing joints of the human skeleton, such as the hip, knee, and ankle joints, are synovial joints that are formed by the joint capsule wrapping the articular surface and the joint cavity, and can perform extension, flexion, and rotation activities within a certain range. The cartilage tissue covering the surface of this type of articular surface is rich in Type II collagen fibers and proteoglycans (Proteoglycans) that can absorb a large amount of water molecules, so it has a good effect on relieving and absorbing mechanical pressure. effect. However, because cartilage tissue does not contain blood vessels, the nutrients needed by cells come from the synovial fluid i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0655C12N2509/00
Inventor 丁磊熊刘留周紫晔崔萌王全明邬敏辰
Owner JIANGNAN UNIV
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