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Rapeseed protein-based nanogel and application thereof

A technology of rapeseed protein and nanogel, applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of poor solubility applications, achieve uniform particle size distribution, improve bioavailability, good water resolubility and The effect of biocompatibility

Inactive Publication Date: 2019-11-22
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although rapeseed protein is a high-quality vegetable protein with abundant sources, and has good water absorption, emulsification, foaming and gelling properties, its poor solubility severely limits its application in food and other fields
In the prior art, there is no method to prepare rapeseed protein-based nanogel carrier

Method used

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  • Rapeseed protein-based nanogel and application thereof
  • Rapeseed protein-based nanogel and application thereof
  • Rapeseed protein-based nanogel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation method and properties of stable self-assembled rapeseed protein-based nanogels

[0029] 1. a preparation method of a stable self-assembled rapeseed protein-based nanogel, comprising the following steps:

[0030] (1) According to the ratio of 1 g rapeseed meal per 15 mL of water, add rapeseed meal to deionized water, adjust the pH of the solution to 11.0 with 1M NaOH aqueous solution, and then stir at 45 °C for 2 h. g Centrifuge for 30 min. The supernatant was recovered, the pH of the supernatant was adjusted to 4.5 with 1M hydrochloric acid, and centrifuged at 10,000 g for 30 min to collect the precipitate. The precipitate was washed three times with absolute ethanol, then dispersed in deionized water, adjusted to pH 7.0 with 1M NaOH aqueous solution, and freeze-dried to obtain rapeseed protein.

[0031] (2) Adjust the pH of deionized water to 10 with NaOH solution. According to the ratio of adding 2g rapeseed protein per 100 ml of water, the ra...

Embodiment 2

[0040] Example 2 Application and effect of rapeseed protein-based nanogel in loading curcumin

[0041] 1. Curcumin loaded with rapeseed protein-based nanogels

[0042] The method for loading curcumin with rapeseed protein-based nanogel includes the following steps:

[0043] (1) According to the ratio of adding 1g rapeseed meal per 15ml of water, add rapeseed meal to deionized water, adjust the pH of the solution to 11.0 with 1M NaOH aqueous solution, and then stir at 45 °C for 2 h, the suspension is 10000 g Centrifuge for 30 min. The supernatant was recovered, the pH of the supernatant was adjusted to 4.5 with 1M hydrochloric acid, and centrifuged at 10,000 g for 30 min to collect the precipitate. The precipitate was washed three times with absolute ethanol, then dispersed in deionized water, adjusted to pH 7.0 with 1M NaOH aqueous solution, and freeze-dried to obtain rapeseed protein.

[0044] (2) Adjust the pH of deionized water to 10 with NaOH solution. According to the...

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Abstract

The invention provides rapeseed protein-based nanogel and application thereof, and belongs to the field of nano functional materials. The rapeseed protein-based nanogel is prepared by the following method: performing acylation reaction on rapeseed protein and succinic anhydride to obtain acylated rapeseed protein; adjusting the pH value of the acylated rapeseed protein aqueous solution, heating and quickly cooling the solution. The invention also provides curcumin-loaded rapeseed protein-based nanogel which is prepared by the following method: carrying out acylation reaction on rapeseed protein and succinic anhydride to obtain acylated rapeseed protein; adjusting the pH value of the acylated rapeseed protein aqueous solution, dropwise adding a curcumin solution into the protein solution, and heating and rapidly cooling the mixture; and removing free curcumin to obtain the curcumin-loaded rapeseed protein-based nanogel. The nanogel has good water resolubility and biocompatibility, is uniform in size distribution and high in stability, can efficiently embed hydrophobic active substance curcumin, remarkably improves the anti-tumor activity of curcumin, and improves the bioavailabilityof curcumin.

Description

technical field [0001] The invention belongs to the field of nano-functional material preparation and its application, in particular to a rapeseed protein-based nano-gel, a preparation method and its application. Background technique [0002] Natural polymers have shown great potential in the fields of medicine, nutrition and food. As a nano-delivery system, plant-derived degradable and biocompatible proteins have attracted more and more researchers due to their unique ability to form nano-scale gel carriers and entrap various hydrophobic and hydrophilic active substances. s concern. [0003] my country is the world's largest producer of rapeseed, and its total output ranks first in the world, accounting for about 30% of the world's total output. After rapeseed oil is made, about 55% of rapeseed meal can be obtained. In 2018, the output of rapeseed meal in my country was as high as 10.39 million tons. Rapeseed meal contains about 40% protein, of which the content of methi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00C08K5/132C08H1/00A61K9/06A61K31/12A61K47/42B82Y5/00A61P35/00
CPCA61K9/06A61K31/12A61K47/42A61P35/00B82Y5/00C08H1/00C08J3/075C08J2389/00C08K5/132
Inventor 王志高陈冲鞠兴荣何荣邹智鹏汪玉娇陈文烨
Owner NANJING UNIV OF FINANCE & ECONOMICS
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