Sample pretreatment apparatus and detection method for carcinogenic N-nitrosamine compounds in meat products and aquatic products

Abstract

The present invention provides a sample pretreatment apparatus and a detection method. The apparatus comprises a water vapor generation device 1, a water vapor introduction pipeline 2, a water vapor introduction sample bottom pipeline 3, a sample distillation extraction device 4, wherein the sample distillation extraction device comprises a ground sample storage flask 5, a ground double-layer glass buffer connection spherical tube 6, a serpentine condensation tube 7, a distillate receiving tube 8, a second ground buffer glass interface 9, a distillate collection bottle 10, an electronic temperature control device 11 and a circulating condensate unit 12. The apparatus simultaneously distills different samples by multichannel water vapor for the extraction of carcinogenic N-nitrosamine compounds from samples, and for the verification and control of carcinogenic N-nitrosamine compounds in meat products and aquatic products by gas chromatography-thermal mass spectrometry and gas chromatography-tandem mass spectrometry.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a sample pretreatment device for carcinogenic N-nitrosamine compounds in meat products and aquatic products. Background technique [0002] At present, the instrumental detection of N-nitrosamine compounds mainly adopts gas chromatography-thermal energy analyzer method, gas chromatography-mass spectrometry, liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry. The sample pretreatment techniques mainly used steam distillation, QuEChERS method, solid phase microextraction (SPME), SPE purification concentration method and supercritical fluid extraction method. The steam distillation method is adopted by GB 5009.26-2016 because it can obtain a cleaner extract, effectively reduce impurities and interference, and protect the instrument inlet and chromatographic column. However, the steam distillation device in the laboratory generally uses customiz...

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Problems solved by technology

The steam distillation method is adopted by GB 5009.26-2016 because it can obtain a cleaner extract, effectively reduce impurities and interference, and protect the instrument inlet and chromatographic column. However, the steam distillation device in the laboratory generally uses customized glassware In the way of building by yourself, the distillation process of samples can only be completed one by one, which is inefficient, and the device is easy to seal and leak air during the distillation process, resulting in a decrease in recovery rate; and the traditional water vapor generating glassware is not safe even if there is a safety tube. It is easy to break due to excessive pressure during the heating process, causing experiment failure and personal injury to experimenters

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