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Application of collagen implant in preparing glaucoma surgery auxiliary recovery substance

A technology of collagen and glaucoma, applied in the field of biomedical materials, can solve problems such as thinning of the conjunctiva, achieve the effects of reducing the degree of paralysis, inhibiting conjunctival shrinkage, and high activity

Inactive Publication Date: 2019-10-11
嘉兴市爵拓科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some drugs and methods have been used clinically, such as mitomycin-C (MMC) or anticancer drug 5-fluorouracil (5-FU), are often used in trabeculectomy or filtering surgery, although MMC is used as a glaucoma surgery Combined treatment is the current domestic standard treatment, but the complication rate of combined treatment with MMC after glaucoma trabeculectomy is 58.5%
And MMC may cause many side effects (including low intraocular pressure, shallow anterior chamber, conjunctival thinning, endophthalmitis, etc.)
Obviously, these drugs and methods also have shortcomings, and if they are used inadvertently, they will bring some negative effects. Finding more effective and safer drugs and methods is still one of the current glaucoma research directions

Method used

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  • Application of collagen implant in preparing glaucoma surgery auxiliary recovery substance
  • Application of collagen implant in preparing glaucoma surgery auxiliary recovery substance
  • Application of collagen implant in preparing glaucoma surgery auxiliary recovery substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The purpose of collagen implant in the preparation of glaucoma surgery auxiliary restoration, wherein the collagen is cowhide purified collagen; the restoration is a spongy collagen-chitosan matrix with a three-dimensional pore structure, and the matrix pore structure pore size distribution is 150-450 μm; the preparation of the above-mentioned complex does not use the cytotoxic component glutaraldehyde as a cross-linking agent. When the restoration substance matrix is ​​placed in individual eyes, it can effectively promote cells, aqueous humor, etc. to pass through the matrix or discretely proliferate in the matrix, interfere with the collagen deposition pattern, maintain normal intraocular pressure, and inhibit conjunctival scarring and conjunctival contraction, Due to the need for the safety of the matrix, it is not ideal or preferred to use glutaraldehyde as a cross-linking agent, so components with low cytotoxicity are selected to prepare the reconstituted product. ...

Embodiment 2

[0049] The preparation method of the auxiliary restoration product for glaucoma surgery, the specific steps are as follows:

[0050] 1) Mechanically depilate the cowhide, remove dandruff, subcutaneous meat and fat, and after rinsing, cut the hide into small pieces of 1cm×1cm, and use 5% Na 2 CO 3 Degreasing the aqueous solution for 16 hours to obtain degreased cowhide, then adding a 5% NaCl solution with a mass fraction of 1:15 to remove salt-soluble non-collagen components, and finally rinsing with distilled water several times to obtain clean cowhide particles, and then Add cowhide particles to 0.5mol / L acetic acid medium with a weight ratio of 1:10 to swell for 12 hours, and then use a high-speed tissue grinder to homogenize at a speed of 12000r / min and a temperature of 15°C to obtain pretreatment Cowhide homogenate;

[0051] 2) Add acetic acid solution with a concentration of 1mol / L to the homogenate at a weight ratio of 1:18. After mixing evenly, add 6000U / g of pepsin t...

Embodiment 3

[0055] The differences between this embodiment and embodiment 2 are:

[0056] Step 2) Add acetic acid solution with a concentration of 1mol / L to the homogenate at a ratio of 1:18 by weight ratio of material to liquid. After mixing evenly, add 6000U / g of pepsin to adjust the pH of the system to 1.8. ℃, under the condition of auxiliary microwave power 400W, extract for 7.5h, and obtain the enzymatic hydrolysis solution. The above-mentioned acetic acid solution contains 0.05mM calcium glycerophosphate and 0.05mM D-3-hydroxybutyric acid. Hydroxybutyric acid introduces phosphate ions, calcium ions, etc. into the system to change the activation energy of the system, reduce the internal viscosity of the substrate, and then use the amphoteric electrolytic properties of the enzyme to penetrate deep into the hydrophobic and non-polar region of the enzyme structure, and lower the medium in the enzyme. The catalytic group in the electrical environment is protected to ensure the high activ...

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Abstract

The invention provides application of a collagen implant in preparing a glaucoma surgery auxiliary recovery substance, and belongs to the field of biomedical materials. The collagen is a cowhide purified collagen, and the recovery substance is a sponge-shaped collagen-chitosan matrix with a three-dimensional pore structure and the pore size distribution of 150-450 microns and serves as a surgery auxiliary recovery substance for glaucoma trabeculectomy. The recovery substance can promote the penetration of cells or aqueous humor or the discrete proliferation in the cells or aqueous humor to maintain low intraocular pressure and reduce the expression quantities of basic fibroblast growth factors, transforming growth factors and fibrous connective tissue growth factors to reduce the scarringdegree of a filtering tract; a preparation method of the recovery substance has the advantages that glutaraldehyde is not adopted as a cross-linking agent, the number of exposed amino groups in the chitosan structure can be reduced, the problem that the anti-scarring effect is not obvious due to the adhesion of excessive amino groups and the tissue is solved, the protective hydration function of chitosan chains is destroyed, pore channels in the matrix are smaller and compact, and the water absorption performance is improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to the use of collagen implants in preparing auxiliary restorations for glaucoma operations. Background technique [0002] Under normal circumstances, aqueous humor (aqueous humor) is generated from the ciliary body, passes through the trabecular meshwork, flows to the canal of Schlemm, and then flows into the blood for recovery. Glaucoma patients refer to the blockage of this circulation channel, resulting in an abnormal increase in intraocular pressure (Intra-ocular Pressure, IOP) (normal intraocular pressure is 10-21mmHg, glaucoma patients IOP>21mmHg), this high intraocular pressure will compress the retina and optic nerve , It may also cause local blood vessel collapse, affect the normal blood supply of the eyeball, cause optic nerve atrophy and visual field defect, clinically known as glaucoma. [0003] Glaucoma is a kind of optic nerve damage disease caused ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L27/20A61L27/24A61L27/56A61L27/58
CPCA61L27/20A61L27/24A61L27/56A61L27/58A61L31/042A61L31/044A61L31/146A61L31/148C08L89/00C08L5/08
Inventor 刘慧芬孟祥龙宋艳丽
Owner 嘉兴市爵拓科技有限公司
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