Detection method of heavy metal lead ion

A detection method and lead ion technology are applied in the detection field of heavy metal lead ions, which can solve the problems of inability to meet detection requirements, children's blood lead exceeding the standard, and complicated pretreatment, so as to achieve superiority, reduce influence, and reduce experimental errors. Effect

Active Publication Date: 2022-04-01
HUBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In January 2011, more than 100 local children in Huaining County, Anhui Province had blood lead exceeding the standard. Subsequent investigations found that it was because of Borui Power Co., Ltd.’s overtime and illegal production, and the sanitary protection distance between the factory and the residential area did not meet the standard
However, in the implementation of this patent, the pretreatment of samples is relatively complicated, and a large-scale precision instrument atomic absorption instrument is required, so it cannot meet the detection requirements, and a fast and simple measurement method needs to be developed

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  • Detection method of heavy metal lead ion
  • Detection method of heavy metal lead ion
  • Detection method of heavy metal lead ion

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Embodiment 1

[0052] The present invention detects the preparation method of the ratio fluorescent biological probe of heavy metal lead ion, comprising:

[0053] In step (6), 0.5 mL of aminated magnetic beads, 2 mL of nano-gold solution, ultrasonic vibration for 3 minutes, magnetic separation to remove the supernatant, and 0.5 mL of citric acid buffer solution, the concentration of SH-DNA is 20 μM, and the amount of addition is 25.1 μL , incubate for 30 min, add 500 μL of red light CdSe@ZnS quantum dots and incubate for 2 h to prepare a red fluorescent probe. Under ultraviolet light, the solution shows red fluorescence.

[0054] In step (7), 100 μL of green CdSe@ZnS quantum dots modified with mercaptopropionic acid was added to 500 μL of DC for activation, and then mixed with 36 μL of DNA with a concentration of 20 μM terminally modified amino groups, and incubated for 2 h to prepare a green fluorescent probe. The solution showed green fluorescence.

[0055] In step (8), take 150 μL of red...

Embodiment 2

[0057] The preparation of the ratiometric fluorescent biological probe specifically includes the following steps:

[0058] Step (1), FeCl 3 ·6H 2 O is 0.81g, ethylene glycol solution is 30mL, polyacrylic acid is 20g, deionized water is 500μL and urea is 1.80g, after stirring to fully dissolve, the ultrasonic time is 10 minutes to mix, and the steel hydrotherm with polytetrafluoroethylene lining is added The volume of the kettle is 50 mL, the reaction temperature is 200° C., the reaction time is 12 hours, and then cooled to room temperature.

[0059] Step (2), get Fe in step 1.1 3 o 4 Nanomaterials are 2mL, ultrasonically dispersed, under the condition of rotating speed of 500rpm, add 0.75mL of 25% ammonia water and stir for 5 minutes, the amount of added tetraethyl orthosilicate (TEOS) is 80μL (TEOS: water ratio 1:1), The sample volume was 10 μL / 10 min and the reaction was stirred continuously at room temperature for 12 hours.

[0060]Step (3), take 500 μL of the solution...

Embodiment 3

[0064] The ratiometric fluorescent biological probe constructed by the present invention is used to detect the content of lead ions in standard samples, and construct a standard curve for detection of lead ions. The specific steps are as follows:

[0065] C-2 The ratio fluorescent bioprobe prepared by the above method is treated with the following 9 standard lead ion solutions respectively, and the molar concentrations are respectively a) 10 -5 μg / mL; b) 10 -4 μg / mL; c) 10 -3 μg / mL; d) 3×10 -3 μg / mL; e) 6×10 -3 μg / mL; f) 10 -2 μg / mL; g) 3×10 -2 μg / mL; h) 6×10 -2 μg / mL; i) 0.1 μg / mL.

[0066] At room temperature, the pH of the probe solution was adjusted to 3, 5, 7, 9, 11 respectively, and the incubation time was 0-600s. It was found that when the pH of the probe solution was 9 (such as Figure 5 ), the incubation time is 300s (such as Figure 4 ). Fluorescence Intensity I of Ratiometric Fluorescent Bioprobes 540 / I 630 The ratio of the logarithm of the lead ion conc...

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Abstract

The invention discloses a detection method for heavy metal lead ions. Nano-gold is modified on the surface of aminated magnetic beads to form a fluorescence quencher, and then the nano-gold is connected with sulfhydryl DNase through an Au-S bond, and the red color is modified The quantum dots form a red fluorescent probe, which is then combined with the substrate chain modified at the other end of the green quantum dot through complementary base pairing, finally forming a ratiometric fluorescent bioprobe. Under the action of DNA, Pb 2+ It can recognize and cut the substrate chain, thereby changing the distance between the green quantum dots and the gold nanoparticles, realizing the quenching and recovery of green fluorescence, and finally realizing the change of the red-green fluorescence ratio caused by the change of the green fluorescence intensity. 2+ quantitative detection. The method is simple and convenient. It adopts the dual-signal detection mode, which effectively reduces the influence of the background environment on the detection results and reduces the experimental error. The method is easy to operate and low in detection cost. Realize the quantitative detection of target objects.

Description

technical field [0001] The invention relates to the technical field of chemical testing and analysis, in particular to a detection method for heavy metal lead ions. Background technique [0002] Lead is a soft and malleable heavy metal, originally blue-white in color, and poisonous. It is the most abundant and most toxic physiological toxin and neurotoxin, and it can also cause poisoning in very small amounts. The ideal lead content in human blood is 0. The accumulation of lead in the human body will disturb the nervous system, digestive system, immune system, and blood system, and even cause the death of brain cells, seriously affecting intellectual development, especially for children. More serious, there will be phenomena such as hyperactivity and learning disabilities, which will cause symptoms such as irritability, personality changes, and depression in people's personality and psychology. At present, it is mainly used in construction, lead-acid batteries, warheads, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N1/28
CPCG01N21/6428G01N21/643G01N21/6486G01N1/28G01N2021/6432
Inventor 吴龙李明月刘静敏陈小强
Owner HUBEI UNIV OF TECH
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