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A new genus strain with alginolytic ability and its application to Phaeocystis globosa

A technology of Phaeocystis globosa and strains, which is applied in the field of environmental microorganisms and can solve problems such as the negative impact of benthic organisms, non-red tide biological damage, and secondary environmental pollution.

Active Publication Date: 2022-05-17
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the physical method is effective quickly and will not cause harm to the ecological environment, it is very likely to have a negative impact on benthic organisms and cannot fundamentally control red tides
The chemical method is currently the most effective method in comparison, but it is easy to cause secondary pollution to the environment, and it cannot only affect red tide organisms in a targeted manner. It will kill red tide organisms and also cause a certain degree of damage to non-red tide organisms. injury on

Method used

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  • A new genus strain with alginolytic ability and its application to Phaeocystis globosa
  • A new genus strain with alginolytic ability and its application to Phaeocystis globosa

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Experimental program
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Embodiment 1

[0025] Embodiment 1, the separation and preparation of new bacterial strain M0103 of the present invention

[0026] Take the leaching solution of Lime snail tissue, dilute it and spread it on ISP4 solid medium, cultivate it at 30°C for 21 days, pick a single colony, and then streak and purify it.

[0027] Sent to the China Center for Type Culture Collection for preservation, and its preservation number is CCTCC NO:M 2019404.

Embodiment 2

[0028] Embodiment 2, the appearance characteristic of new bacterial strain M0103 of the present invention

[0029] Take a single colony of bacterial strain M0103, transfer it to ISP2+2.6% sea salt solid medium (agar), and cultivate it in a constant temperature incubator at 30°C for 48 hours, and observe the size, color, edge, protrusion, smoothness, and Viscosity, transparency and other morphological characteristics.

[0030] The results showed that the strain M0103 formed neat, slightly raised, smooth, opaque, milky white round colonies with a diameter of about 2-3mm on the solid medium of ISP2+2.6% sea salt.

Embodiment 3

[0031] Embodiment 3, the growth characteristic of new bacterial strain M0103 of the present invention

[0032] Fresh cultures cultivated on ISP2+2.6% sea salt solid medium for 24 hours were picked, inoculated into R2A liquid medium, and cultured on a shaker at 37° C. for 36 hours as seeds.

[0033] The composition of ISP2+2.6% sea salt solid medium: yeast extract powder 2.0g / L, malt extract powder 2.0g / L, glucose 2.0g / L, sea salt 26.0g / L, 1.4% agar powder, deionized water 1000mL.

[0034] R2A solid medium: yeast powder 0.5g / L, tryptone 0.5g / L, casamino acid 0.5g / L, glucose 0.5g / L, soluble starch 0.5g / L, dipotassium hydrogen phosphate 0.3g / L, seven Magnesium sulfate water 0.05g / L, sodium pyruvate 0.3g / L, agar 13.0g / L, deionized water 1000mL.

[0035] 1. Growth temperature

[0036] The culture was inoculated on ISP2+2.6% sea salt solid medium, cultured at 10°C, 15°C, 20°C, 25°C, 30°C, 37°C, 40°C and 45°C, and three parallels were made for each temperature gradient. , and obse...

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Abstract

The invention discloses a novel genus strain with algae-dissolving ability, the preservation number is CCTCC NO: M 2019404, and the classification name is Mesobaculum littorinae M0103. The bacterial strain is simple to screen and easy to cultivate, and the inventor has also established a corresponding cultivation method. Studies have shown that the M0103 strain has a good algae-dissolving effect, and its fermentation liquid has a good inhibitory and killing effect on the dominant algal species Phaeocystis globosa in the Beibu Gulf sea area, and the algae-dissolving rate reaches more than 90% under 24h treatment conditions. Since the M0103 strain comes from snails in the Beibu Gulf, it also has the characteristics of good ecological safety and will not cause secondary pollution during use. The discovery of this strain has enriched the available microbial resources in my country, and its algae removal effect is stable, efficient, and environmentally friendly, and it has a good application prospect in controlling the red tide of Phaeocystis globosa.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to a new genus strain with algae-dissolving ability and its application to Phaeocystis globosa. Background technique [0002] Red tide refers to the sudden proliferation and accumulation of some phytoplankton, protozoa or bacteria in the marine water body under certain environmental conditions, causing the water body to change color within a certain range and within a period of time. Red tides are very harmful, mainly reflected in the following three aspects: First, red tides damage the ecological balance of the ocean. In the early stage of red tide, high chlorophyll, high dissolved oxygen, and high chemical oxygen consumption appear in the water body, causing drastic changes in the surrounding marine environment and destroying the ecological balance of the water body. Second, red tides damage marine fisheries and aquatic resources. In the early stage of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P13/00C12R1/01
CPCC12N1/20A01N63/00C12R2001/01C12N1/205
Inventor 李菲覃仙玲陈波潘信利姜发军李喆
Owner GUANGXI ACAD OF SCI
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