PLGF magnetic particle chemiluminiscence kit and detection method thereof

Abstract

The present invention discloses a PLGF (Placental Growth Factor) magnetic particle chemiluminiscence kit and a detection method thereof. The kit comprises a placental growth factor antibody with biotin labeling, streptavidin magnetic particle suspension liquid, a placental growth factor antibody with acridinium ester labeling, a calibration product, cleanout fluid, a diluent, chemiluminiscence pre-excitation liquid A, and chemiluminiscence pre-excitation liquid B. The streptavidin and the biotin have a high-specificity combining capacity, the high-purity antibody of the streptavidin and the biotin labeling is subjected to specific binding through a non covalent bond and has a cascade amplification effect. Besides, an acridinium ester chemiluminescent system is adopted, and the acridinium ester does not need a catalyst and can emit light when being put in a H2O2 solution with no need for a catalytic process and an enhancer. The sensitivity is higher, the reaction time is short, the operation is simple, and the anti-interference performance is high.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a PLGF magnetic particle chemiluminescence kit and a detection method thereof. Background technique [0002] Placental growth factor (PLGF) was first isolated and purified from human placental cDNA library by Maglione et al. in 1991. PLGF is mainly synthesized by syncytiotrophoblast cells and can bind to tyrosinase receptors located in trophoblast cells and vascular endothelial cells. It is a protein that has autocrine effects on trophoblast cell functions and paracrine effects on angiogenesis. PLGF has a unique regulatory effect on the function of trophoblast cells and endothelial cells, and can promote angiogenesis. Detecting the blood PLGF level of pregnant women can be used clinically to identify the oxygen supply pressure of placental syncytiotrophoblast cells, and to predict, identify and monitor treatment of preeclampsia. [0003] So far, the methods used to detect plac...

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Problems solved by technology

[0003] So far, the methods used to detect placental growth factor residues in human serum mainly include: enzyme-linked immunoassay (ELISA). However, although the detection price of ELISA is low and fast, the sensitivity is not enough, and it is only suitable for the detection of trace substances. Detection and identification; the method adopted in the present invention is the direct chemiluminescence method, and the use of acridinium ester as a chemiluminescent marker has obvious advantages, mainly in that the reaction does not require a catalyst, as long as the alkaline environment can be carried out, the reaction is rapid, and can be completely captured Photons generated by reaction, low background luminescence, high signal-to-noise ratio, less interference factors, good reagent stability, simple system, low cost of excitation solution, acridinium ester is easy to bind with protein, and the photon yield does not decrease after the association