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Establishment method of hplc fingerprint of Zhuang medicine Baihua Jiuliming

A technology of fingerprints and establishment methods, which is applied in the field of establishment of HPLC fingerprints of Zhuang medicine Baihua Jiuliming

Active Publication Date: 2021-09-14
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there has not been any report on the research on the fingerprint of Zhuang medicine Baihua Jiuli at home and abroad. The present invention conducts HPLC fingerprint research on the Zhuang medicine Baihua Jiuli, which makes up for the blank under the quality standard of Baihua Jiuliming, and is the medicinal material of Baihua Jiuliming. It provides a basis for the quality evaluation of Baihuajiuliming, and provides a reference for more scientific, reasonable and standardized use of Baihuajiuliming and improving the safety of its clinical medication.

Method used

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  • Establishment method of hplc fingerprint of Zhuang medicine Baihua Jiuliming
  • Establishment method of hplc fingerprint of Zhuang medicine Baihua Jiuliming
  • Establishment method of hplc fingerprint of Zhuang medicine Baihua Jiuliming

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1 Experimental materials and instruments

[0040] 1.1 Medicinal materials: place of origin and harvest time are shown in Table 3.

[0041] Table 3 Origin and harvest time of Baihua Jiuliming medicinal materials

[0042] Medicinal No. Source of medicinal materials (origin) harvest time 1 whith 2014.11 2 Nanning peak 2014.11 3 Bama 2014.11 4 Nanning peak 2014.03 5 Heaven waits 2015.11 6 Bama 2015.11 7 Beiliu 2015.11 8 Napo 2013.06 9 Chongzuo 2013.11 10 Nanning Tiger Ridge 2015.11 11 Beiliu 2016.10 12 Fangcheng 2015.11 13 Lu Chuan 2016.10

[0043] 1.2 Instruments: Agilent1260 high performance liquid chromatography (including G1311C quaternary pump, G1329B autosampler, G1316A column oven, G1315D DAD detector, Agilengt1260 chromatographic workstation (Agilent Technologies, USA)); medium-sized ultrasonic cleaner (Elma Schmidbauer GmbH, P300H); electronic balance (Sart...

Embodiment 2

[0054] Get 13 batches of Baihuajiuliming medicinal materials (medicinal material numbering 1-13), prepare need testing solution by the method under "2.3", measure by the chromatographic conditions under the "2.1" item and the measuring method under the "2.4" item, obtain 13 Batch sample HPLC fingerprint overlay, such as image 3 shown. Through the comparison of these 13 batches of HPLC spectra, the similarity evaluation is carried out to determine the characteristic common peaks: the fingerprints have 10 characteristic common peaks, and the retention time, the average value of the peak area and the proportion of the total peak area are summarized as follows:

[0055] Peak No. 1, retention time 2.726min, RSD% 0.66%, peak area 156.555, RSD% 47.01%; accounting for 1.520% of the total peak area;

[0056] Peak No. 2, retention time 3.483min, RSD% 0.17%, peak area 674.258, RSD% 90.55%; accounting for 6.544% of the total peak area;

[0057] Peak No. 3, retention time 4.599min, RSD%...

Embodiment 3

[0072] 3 Methodological investigation

[0073] 3.1 Precision experiment

[0074] Accurately draw the same test solution (prepared from Baihuajiuliming herb powder No. 1 according to the method under "2.3"), and inject 6 samples according to the chromatographic conditions under "2.1" and the determination method under "2.4" Second, record the fingerprint, see Figure 4 . Taking peak No. 6 (caffeic acid) as the reference peak, the relative retention time RSD value of each peak is calculated to be 0.01%-0.48%, and the relative peak area RSD value of each peak is 0.3%-2.04%. The correlation coefficients are shown in Table 6.

[0075] Table 6 Similarity results of precision experiment

[0076]

[0077] It can be seen from Table 6 that the correlation coefficients of precision experiments are all greater than 0.95, and the similarity counting results show that the instrument has good precision and meets the requirements of fingerprint detection.

[0078] 3.2 Stability experi...

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Abstract

The invention belongs to the field of fingerprints of traditional Chinese medicinal materials, and in particular relates to a method for establishing the HPLC fingerprints of Zhuang medicine Baihuajiuliming. The method for establishing the HPLC fingerprint of Zhuang medicine Baihuajiuliming uses caffeic acid, the main component of Baihuajiuliming, as a reference, and obtains the common pattern fingerprint of Zhuang medicine Baihuajiuliming accordingly. There are 10 chromatographic peaks in the fingerprint spectrum, which can fully reflect the chemical components in the medicinal material Baihua Jiuliming. The amount of information is relatively rich, and the method has good reproducibility. It provides a more powerful theoretical basis for controlling the quality of medicinal materials and identifying the advantages and disadvantages of medicinal materials. The quality control and authenticity identification level of the medicine Baihua Jiuliming has realized the rapid and accurate identification of the authenticity of Baihua Jiuliming.

Description

【Technical field】 [0001] The invention relates to the field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing the HPLC fingerprints of Zhuang medicine Baihuajiuliming. 【Background technique】 [0002] Baihua Jiuliming is the above-ground part of Blumea megacephala (Randeria) Chang et Tseng in the family Asteraceae. It is produced in Yunnan, Sichuan, Guizhou, Guangxi, Guangdong, southern Hunan, southern Jiangxi, Fujian and Taiwan. Baihuajiuliming is commonly used decocted in water to treat postpartum bleeding among the Zhuang people in Guangxi. [0003] Traditional Chinese medicine fingerprinting (fingerprinting) is developed by borrowing DNA fingerprinting. The first developed is the chromatographic fingerprint of the chemical constituents of traditional Chinese medicine, especially the high performance liquid chromatography (HPLC) fingerprint. HPLC has a high degree of resolution and can separate complex chemical compone...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8631G01N30/8686G01N30/88G01N2030/8809
Inventor 宁小清朱智德张文涛银胜高黄薏霏谈远锋韦威
Owner GUANGXI UNIV OF CHINESE MEDICINE
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