Reagent kit for detecting enterobacter aerogenes

Abstract

The invention provides a reagent kit for detecting enterobacter aerogenes. The reagent kit contains a guide RNA specifically targeted to an enterobacter aerogenes rpoB gene, an amplification primer pair, a hydration TwistAmp basic kit reaction drying ball, LbCas12a protein, a single-stranded DNA probe (ssDNA), a Ribonuclease inhibitor and a buffer solution. When the reagent kit is used for detecting the enterobacter aerogenes, the detection specificity is high, and the enterobacter aerogenes can be distinguished from other enterobacter bacteria including enterobacter cloacae, enterobacter gergoviae and enterobacter sakazakii. Besides, when the RNA sequence is used for detection, time consumption is about 1 hour, a PCR instrument is not needed, requirements for equipment are low and are notably lower than those by a traditional bacteria culture method or a PCR sequencing method, and the clinical use value is high.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for detection of Enterobacter aerogenes. This kit can be used for rapid detection of Enterobacter aerogenes. Background technique [0002] Enterobacter aerogenes is a common bacterial group among Gram-negative bacteria that infects humans, and it brings a huge burden to patients and society. The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Enterobacter aerogenes from other Enterobacter bacteria due to the lack of specific primers, so combined It can only be identified by first-generation sequencing, which is costly and takes several days to produce results. During this period, clinicians can only use medicines based on experience because they do not obtain test results, which often leads to delays in disease treatment. Therefore, how to quickly detect Ente...

AI Technical Summary

Problems solved by technology

The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Enterobacter aerogenes from other Enterobacter bacteria due to the lack of specific primers, so combined Only generation sequencing can be identified, the cost is high, and it takes several days to get the results

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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