Exosome drug carrying system and applications thereof in spinal cord injury repairing

A technology for spinal cord injury and exosomes, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, and can solve problems such as neurotoxicity, low bioavailability, and poor biocompatibility. , to achieve the effect of promoting the recovery of behavioral function, reducing diffusion loss, and favoring differentiation

Active Publication Date: 2019-09-13
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, as a hydrophobic molecule, paclitaxel has poor water solubility and low bioavailability
Clinically, it is often dissolved in a mixed solvent of equal volumes of polyoxyethylene castor oil (EL) and ethanol, resulting in poor biocompatibility and various side effects, such as severe hypersensitivity, myelosuppression, neurotoxicity
In addition, when drug is administered in vivo, due to the free diffusion activity of drug molecules and the washout effect of body fluids, the drug is released rapidly, which greatly limits its therapeutic effect.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Exosome drug carrying system and applications thereof in spinal cord injury repairing
  • Exosome drug carrying system and applications thereof in spinal cord injury repairing
  • Exosome drug carrying system and applications thereof in spinal cord injury repairing

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] Another aspect of the embodiments of the present invention also provides the preparation method of the aforementioned exosome drug-carrying system, which includes: blending and extruding drug molecules with exosomes, so that the drug molecules are entrapped in the exosomes, Formation of exosome drug delivery system.

[0035] Further, the extruding is through an extruder with a nano-sized aperture, and the number of extruding is preferably an odd number, and then excess paclitaxel is removed by ultrafiltration.

[0036] Further, the exosomes of the mesenchymal stem cells are obtained by the following method:

[0037] The mesenchymal stem cells were cultured by conventional culture methods. At the P3 generation, the medium was replaced with serum-free exosomes, and the purified medium was collected to obtain exosomes of human umbilical cord mesenchymal stem cells.

[0038] In one embodiment of the present invention, get the P2 generation cell of logarithmic growth phase,...

Embodiment 1

[0046] 1. Isolation and characterization of exosomes from human umbilical cord mesenchymal stem cells:

[0047] The mesenchymal stem cells were cultured by conventional culture methods, and replaced with serum-free exosome medium at the P3 generation, and the purified medium was collected to obtain human umbilical cord mesenchymal stem cell exosomes.

[0048]Take the P2 generation cells in the logarithmic growth phase and culture them at 175cm 2 In the cell culture flask, when the cell proliferation and confluence reach 70-80%, routine subculture is carried out according to the ratio of 1:4. The P3 generation cells were cultured in a medium without exosome serum, and the cell culture supernatant was collected. The cell supernatant was centrifuged at 300 g for 10 min at 4°C to remove free cells. Transfer the supernatant to another centrifuge tube and centrifuge at 2000g for 10min at 4°C to remove cell debris. Transfer the supernatant to another centrifuge tube, centrifuge at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an exosome drug carrying system and applications thereof in spinal cord injury repairing. The system includes an exosome and drug molecules encapsulated in the exosome; the drug molecules include paclitaxel and enter the drug carrying exosome through an extruding mode; and the exosome is derived from mesenchymal stem cells. An exosome drug carrier can encapsulate a small molecule chemical drug into the exosome, so that the diffusion loss of drugs and caused toxic and side effects can be reduced; and during the application of the spinal cord injuries of rats, the differentiation of endogenous neural stem cells to neurons can be induced, and the recovery of the behavior functions of the rats can be promoted.

Description

technical field [0001] The invention belongs to the technical fields of cell biology, molecular biology and drug research and development, and specifically relates to an exosome drug-carrying system and its construction method, as well as its application in spinal cord injury repair. Background technique [0002] Spinal cord injury is a serious injury to the central nervous system. After injury, a large number of neurons and axons are lost, and it will cause a series of pathological changes, including vascular system breakdown, edema, persistent inflammatory response, and scar formation. Due to the production of a large number of inhibitory molecules and the deposition of glial scars, a microenvironment that is not conducive to regeneration is formed at the injury site, resulting in a decrease in the proportion of neural stem cells that differentiate into neurons, which is not conducive to the connection of nerve regeneration. [0003] Paclitaxel (PTX), a clinically approved...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A61K31/337A61P25/28
CPCA61K47/46A61K31/337A61P25/28
Inventor 陈艳艳张璐璐戴建武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap