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Multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof

A technology of Lactobacillus brucei and fermented food, which is applied in the field of microorganisms, can solve the problems of high price and difficulty in obtaining, and achieve the effects of reducing abundance, alleviating constipation, and improving the time for the first black stool

Active Publication Date: 2019-09-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these natural substances are expensive and difficult to obtain. In addition, due to the tolerance of the human body, it is not yet known whether a large amount of intake will cause potential harm to the human body.

Method used

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  • Multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof
  • Multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof
  • Multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1: Lactobacillus Brucella CCFM1053 has good PFOA adsorption capacity

[0072] Bacterial adsorption Lactobacillus buchneri CCFM1053 was purified and activated, inoculated in MRS liquid medium at 1% (v / v) inoculum, and cultured at 37°C for 18 hours. Then centrifuge at 8000r / min for 5min to collect the bacteria, take the precipitate and clean it with normal saline, then continue to centrifuge at 8000r / min for 5min to remove the precipitate to obtain live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 10mg / LPFOA solution, and the final bacterial concentration reached 1g dry bacteria / L (the wet bacteria were resuspended in PFOA-free ultrapure water as a blank control). Use 0.1M NaOH or HCl solution to quickly adjust the pH of the PFOA solution containing bacteria to 3.0, add a small amount of NaOH or HCl (less than 0.5ml), and its ionic strength has negligible influence on PFOA adsorption. Subsequently, the 250ml Erlenmeyer flask contain...

Embodiment 2

[0077] Example 2: Lactobacillus brucelli CCFM1053 can significantly improve spleen atrophy in mice exposed to PFOA Corticosteroid intervention group, Lactobacillus brucella CCFM1053 intervention group, LGG intervention group, each group contains 10 mice, animal grouping and treatment methods are shown in Table 2

[0078] Table 2 Grouping and processing methods of animal experiments

[0079]

[0080] The mice in Example 2 were weighed on the 13th day, then euthanized, the spleen was taken out and weighed wet to calculate the organ coefficient, and the organ coefficient of the mouse spleen was calculated according to the following formula:

[0081] Spleen organ coefficient = wet weight of spleen / weight of mouse before euthanasia

[0082] Experimental results such as figure 2 As shown, the results show that administration of Lactobacillus brucelli CCFM1053 can significantly reverse the spleen atrophy of mice caused by PFOA exposure.

Embodiment 4

[0083] Example 4: Lactobacillus brucelli CCFM1053 can significantly reduce the levels of ALT, AST and γ-GT in the serum of PFOA exposed mice

[0084] The serum in Example 2 was taken, and the contents of ALT, AST and γ-GT in the serum were detected by an automatic biochemical analyzer. ALT mainly exists in the soluble part of the hepatocyte protoplasm, and the increase of ALT activity indicates that the hepatocytes are destroyed and the permeability of the cell membrane is enhanced. AST mainly exists in the mitochondria of liver cells, and the increase of AST activity indicates mitochondrial damage. The experimental results show( image 3), taking Lactobacillus brucelli CCFM1053 can significantly reduce the levels of ALT, AST and γ-GT in the serum of PFOA-exposed mice. It shows that taking Lactobacillus brucella CCFM1053 can significantly alleviate the damage of PFOA-induced liver cell membrane structure and function in mice.

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Abstract

The invention discloses multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof. The lactobacillus buchneri CCFM1053 can adsorb PFOA, improve spleen atrophy caused by PFOA exposure, increase content of TNA-alpha in serum after PFOA exposure, increase content of glutamic-pyruvic transaminase, glutamic oxalacetic transaminaseand gamma-glutamyl transpeptidase in serum in mice after PFOA exposure, reduce the content of MDA in liver after PFOA exposure to the normal level, reduce GAH activity, improve intestinal flora disorder, reduce hepatopathy, improve excrement moisture content and first melena discharge time of constipation patients, relieve constipation, improve proliferation of INS-1 cells and expression of MafA genes under the high glucose action and relieve PFOA related diabetes mellitus.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a multifunctional Lactobacillus buchneri CCFM1053 for alleviating the toxic effect of PFOA, fermented food and applications thereof. Background technique [0002] Perfluorinated compounds have been widely used in various industries due to their hydrophobic and oleophobic properties, as well as good thermal stability, chemical stability and biological stability. Used in ready-to-wear (such as waterproof, stain-resistant outdoor clothing) and home textiles (such as carpets, furniture fabrics, etc.), takeaway food containers, personal care products (such as dental floss), and fire fighting foam. Among them, PFOA, as one of the final transformation products of various fluorine compounds, can be enriched along the food chain. PFOA has been detected in various environmental media around the world, such as water, soil, atmosphere, dust, etc., as well as in animals and huma...

Claims

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Application Information

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IPC IPC(8): A23C9/123A23C13/16A23C19/00A23C20/02A23L33/135A23L19/20C12N1/20C12R1/225
CPCA23C9/123A23C20/025A23C13/16A23C19/00A23L19/20A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2200/32A23V2200/30
Inventor 王刚陈卫梁席赵建新张灏
Owner JIANGNAN UNIV
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