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Automatic control method for separation and purification of ginsenoside Rd

A technology for the separation and purification of ginsenosides, which is applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problems that restrict the scientific research of ginsenoside monomers and the clinical application of medicine, and achieve improved repeatability, The effect of increasing economic value and increasing content

Active Publication Date: 2019-08-23
吉林紫鑫参工堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It can be seen that the deficiency of the method for obtaining high-purity ginsenoside Rd monomer has always been the main reason for restricting the scientific research and clinical application of ginsenoside monomer

Method used

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  • Automatic control method for separation and purification of ginsenoside Rd
  • Automatic control method for separation and purification of ginsenoside Rd
  • Automatic control method for separation and purification of ginsenoside Rd

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0140] Adopt reverse-phase high-efficiency chromatographic column separation and purification of ginsenoside extract, 80mL of sample solution (containing ginsenoside extract 80g, wherein, Rg1 content is 10%, Rb1 content is 9%, Rd content is 22%, raw material spectrogram is as follows image 3Shown, wherein, Rg1 peak time is 34.9min, Rb1 peak time is 44.5min, Rd peak time is 53.1min), the eluting solvent is ethanol / water, the ratio of the volume sum of ethanol and ethanol and water is (10~60): 100 (the volume ratio of ethanol and water is increasing in real time, gradient elution), the elution flow rate is 120mL / min, and the chromatographic column used is a reversed-phase high performance liquid chromatography column, which is a dynamic axial compression column, The inner diameter is 50mm and the height is 600mm. The filler is spherical ODS filler with a particle size of 10 microns. The column is packed by wet method. The actual number of separation plates is 12000; the flow rat...

Embodiment 2

[0149] (1) Set the elution parameters according to the chromatographic column used and the target content of saponins Rg1, Rb1, and Rd in the ginsenoside to be eluted;

[0150] The chromatographic column used is a reverse-phase high-performance liquid chromatography column, which is a dynamic axial compression column with an inner diameter of 50 mm and a height of 600 mm. The filler is a spherical ODS filler with a particle size of 10 microns. The column is packed in a wet method. The actual number of separation plates is 12000;

[0151] Elution sample: 80mL of sample solution (containing ginsenoside extract 80g, Rg1 content is 10%, Rb1 content is 9%, Rd content is 22%), and it is loaded into the chromatographic column;

[0152] The elution target is that the content of ginsenoside Rg1 is higher than 40%, or the content of Rb1 is higher than 40%, or the content of ginsenoside Rd is higher than 80%;

[0153] The elution parameters were set as the elution solvent was a mixture ...

Embodiment 3

[0164] (1) Set the elution parameters according to the chromatographic column used and the target content of each saponin Rg1, Rb1, and Rd in the ginsenoside to be eluted;

[0165] The chromatographic column used is a reverse-phase high-performance liquid chromatography column, which is a dynamic axial compression column with an inner diameter of 50 mm and a height of 600 mm. The filler is a spherical ODS filler with a particle size of 10 microns. The column is packed in a wet method. The actual number of separation plates is 12000;

[0166] Elution sample: 80mL of sample solution (containing ginsenoside extract 80g, Rg1 content is 11%, Rb1 content is 8%, Rd content is 21%), and it is loaded into the chromatographic column;

[0167] The elution target is that the content of ginsenoside Rg1 is higher than 40%, or the content of ginsenoside Rb1 is higher than 40%, or the content of ginsenoside Rd is higher than 80%.

[0168] Set the elution parameters as the elution solvent is ...

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Abstract

The invention relates to an automatic control method for separation and purification of ginsenoside Rd. The method adopts a reversed-phase high performance chromatographic column to separate and purify ginsenoside extract. An elution solvent is formed by mixing of ethanol and water, and the elution mode is constant flow velocity or variable flow velocity stable pressure elution, also the content of ginsenoside in the eluent is detected and an adsorption model is combined to adjust the elution parameters to complete elution separation, thus obtaining high purity ginsenoside Rg1, Rb1 and Rd, especially high purity ginsenoside Rd. The automatic control method for separation and purification of ginsenoside Rd provided by the invention has the characteristics of high ginsenoside separation purity, high separation efficiency, solvent saving, energy saving and consumption reduction, safety and environmental protection, improves the economic value of the product, and is conducive to large-scale production.

Description

technical field [0001] The invention relates to the field of reverse-phase column chromatography separation and purification process control of natural product intermediates, in particular to a reverse-phase high-efficiency column chromatography automatic control separation and purification method for ginsenoside Rd. Background technique [0002] Ginseng is a traditional precious Chinese medicinal material in my country. Ginsenoside is the main active ingredient of ginseng. It has the main physiological activity of ginseng. There are more than 50 kinds of ginsenoside monomers that are clearly known; the content in ginseng accounts for about 4%. , has the effects of promoting substance metabolism, improving human immunity, anti-tumor, anti-fatigue, and anti-aging. [0003] Ginsenoside Rd, as an important saponin in ginseng extract, has the function of specifically blocking receptor-dependent calcium ion channels, and has other monomeric saponins in renal function protection, i...

Claims

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Application Information

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IPC IPC(8): C07J17/00B01D15/32B01D15/42
CPCC07J17/005B01D15/325B01D15/426
Inventor 韩明殷警菲陈玮
Owner 吉林紫鑫参工堂生物科技有限公司
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