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Bacteria and algae collaborated method for efficient treatment of aquaculture sewage

A technology of bacteria and algae synergy and breeding sewage, which is applied in the direction of filtration treatment, sedimentation treatment, special treatment goals, etc., can solve the problems of affecting growth, poor stability of treatment effect, loss of algae cells, etc., to increase the generation of air bubbles and promote microalgae Effects of growth, prolongation of contact time

Active Publication Date: 2019-08-23
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microalgal cells are generally smaller than 30 microns, negatively charged, and have a density close to that of water. These characteristics make algal cells in a stable suspension state in water, and it is difficult to achieve natural separation through gravity sedimentation like activated sludge.
As a result, a large amount of algae cells will be lost with the treated water, which not only pollutes the treated water twice, but also makes it difficult to maintain a large amount of biomass in the reactor (generally only 0.2~0.6g / L)
Low culture density leads to low removal efficiency, making the treatment effect less stable
Furthermore, microalgae treatment of livestock and poultry sewage has low tolerance to ammonia nitrogen, which affects growth, resulting in slower removal efficiency of total nitrogen and total phosphorus, and unsatisfactory removal effect on COD

Method used

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  • Bacteria and algae collaborated method for efficient treatment of aquaculture sewage
  • Bacteria and algae collaborated method for efficient treatment of aquaculture sewage
  • Bacteria and algae collaborated method for efficient treatment of aquaculture sewage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Such as figure 1 As shown, the device for bacteria and algae synergistically efficient treatment of aquaculture sewage includes a composite bacteria aeration tank 3 and an air-lift columnar photobioreactor 4 . The water inlet of the compound bacteria aeration tank 3 is connected to the water outlet of the grille tank 2 through a water pipe, and the water outlet of the compound bacteria aeration tank 3 is connected to the water inlet of the air-lift columnar photobioreactor 4 through a water pipe; the air-lift columnar photobioreactor There are 3 devices 4, which are connected in series; the water outlet of the air-lift columnar photobioreactor 4 at the end is connected with the water inlet of the sedimentation tank 5 through a water pipe; the water outlet of the sedimentation tank 5 is connected with the ozone decolorization tank 6 through a water pipe The water inlet of the ozone decolorization pool 6 is connected with the water inlet of the water outlet pool 7; the wa...

Embodiment 2

[0031] Such as Figure 1~5 As shown, the method of cooperating with bacteria and algae to efficiently treat aquaculture sewage: livestock and poultry sewage flows from the raw water pool 1 into the grid pool 2, and the grid pool 2 performs preliminary purification on it to remove large suspended particles. Then it flows into the complex bacteria aeration tank 3, and the synergistic action of autotrophic ammonia oxidizing bacteria, autotrophic nitrifying bacteria and heterotrophic nitrifying bacteria converts ammonia nitrogen in livestock and poultry sewage into nitrate nitrogen. The treated sewage is passed into the air-lift columnar photobioreactor 4 from the water inlet 22, and the microalgae with the preservation number of CCTCC NO: M 2017461 is used to remove nitrogen and phosphorus and accumulate biomass. The airlift columnar photobioreactor 4 has a built-in membrane module 17 as a microalgae attachment material, and the bottom of the airlift columnar photobioreactor 4 is...

Embodiment 3

[0033]Biomass measurement: Measure the OD value of the microalgae liquid in Example 1 at 680nm, and count microalgae at the same time, count the microalgae less than 10 μm with a hemocytometer, and count the cell density with a 0.1mL phytoplankton counting frame for the algae greater than 10 μm (count at least 3 times). The OD value was plotted against the cell density to obtain the absorbance coefficient. Then use the formula (lg(m)=-1.0465ln(x)+4.255, where m is the biomass and x is the absorptivity) to convert the absorptivity into biomass. The biomass accumulation in the microalgae of Example 1 after 15 days of treatment was measured according to the above method.

[0034] Determination of crude protein content: the crude protein content in the microalgae in Example 1 was measured by micro Kjeldahl method after 15 days of treatment.

[0035] Determination of oil content: the content of oil in microalgae in Example 1 was measured by Sudan Black B staining method after 15 ...

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Abstract

The invention discloses a bacteria and algae collaborated method for efficient treatment of aquaculture sewage. Bacteria and algae are compounded for use in combination to treat livestock and poultrysewage. The method specifically includes: adopting autotrophic ammonia oxidizing bacteria, autotrophic nitrification bacteria and heterotrophic nitrification bacteria to convert ammonia nitrogen in livestock and poultry sewage into nitrate nitrogen, then introducing the treated sewage into an airlift columnar photobioreactor, and utilizing the microalgae with a preservation number of CCTCC NO:M 2017461 to remove nitrogen and phosphorus and perform biomass accumulation. The method has the beneficial effects that: through the synergistic action of microbial functional bacteria and microalgae, the method rapidly reduces the nitrogen, phosphorus and other pollutants in livestock and poultry sewage, shortens the hydraulic retention time, improves the microalgae biomass accumulation speed, and can acquire a large number of renewable energy sources while treating livestock and poultry sewage.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a method for cooperating with bacteria and algae to efficiently treat aquaculture sewage. Background technique [0002] The history of sewage treatment using microalgae has been traced back for a long time. As early as the 1950s, Oswald et al. proposed the idea of ​​using microalgae to treat sewage. The growth process of microalgae needs to absorb a large amount of nutrients such as nitrogen and phosphorus, which can directly reduce II / The content of pollutants such as N and P in the tertiary effluent, through indirect effects such as fixing carbon dioxide, generating oxygen, and increasing pH, microalgae can also create environmental conditions that can effectively remove residual organic matter and pathogenic microorganisms in the water. In addition, microalgae also have the ability to absorb harmful substances such as heavy metals, therefore, microalgae has a good potential to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14
CPCC02F1/001C02F1/78C02F3/322C02F3/34C02F7/00C02F9/00C02F2001/007C02F2303/04
Inventor 孙宏汤江武李园成吴逸飞姚晓红王新沈琦
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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