Preparation method for gliadin-chitosan condensate

A gliadin and chitosan technology, applied in food science, food ingredients, applications, etc., can solve the problem of protein loading rate and rheological properties not being optimal, and achieve extended application scope and effect, elastic modulus The effect of increasing the volume and viscosity modulus and improving the stability

Inactive Publication Date: 2019-08-16
GUANGZHOU UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0007] In the above patent, when the pH value is 5.0, the protein is not completely adsorbed on the interface, and the loading rate and rheological properties of the protein are not optimal.

Method used

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  • Preparation method for gliadin-chitosan condensate
  • Preparation method for gliadin-chitosan condensate
  • Preparation method for gliadin-chitosan condensate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] The preparation of gliadin-chitosan aggregate (coacervate) comprises the following steps:

[0032] (1) Preparation of gliadin solution (Gliadin): Weigh a certain amount of Gliadin sample, dissolve it in 75% (v / v) ethanol solution, and stir it with a magnetic stirrer at a medium speed at room temperature to make it fully Dissolve, prepare Gliadin stock solution with a concentration of 2% (w / v), and store in cold storage.

[0033] (2) Configuration of chitosan (CS) solution: Weigh a certain mass of CS, dissolve it in 1% (w / v) acetic acid, stir it with a magnetic stirrer at a medium speed for 15 minutes at room temperature, and then put it in the refrigerator to refrigerate Overnight, in order to promote the hydration of CS, a CS stock solution with a concentration of 0.1% (w / v) was prepared and stored in cold storage.

[0034] (3) Mix the two stock solutions: take 10ml of Gliadin solution, place it on a magnetic stirrer and stir (normal temperature, medium speed), then q...

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Abstract

The invention discloses a preparation method for a gliadin-chitosan condensate. The method comprises the following steps: enabling gliadin to be dissolved in alcoholic solution; preparing chitosan acetic acid solution; pouring gliadin alcoholic solution into the chitosan acetic acid solution and homogenizing, wherein a volume ratio of two types of the solution is 1:7, rotary-evaporating the alcoholic solution, centrifuging, and taking supernatant liquid; and adjusting a pH value of the supernatant liquid to be 7.0, after enabling obtained solution to be mixed with grease, shearing and emulsifying, to obtain the gliadin-chitosan condensate. Stability, amphipathic capacity and protein load rate of the gliadin-chitosan condensate are improved. A cross stress, an elasticity modulus and a viscosity modulus are improved too. Application range and effect are greatly extended.

Description

technical field [0001] The invention belongs to the field of protein-polysaccharide complexes, in particular to a preparation method of gliadin-chitosan coacervate, which can be used for embedding food nutrients in the future. Background technique [0002] Protein-polysaccharide interactions were first discovered when studying the interaction of sulfated polysaccharides with plasma lipoproteins. [0003] In recent years, many teams have been working on the biological significance and industrial application potential of protein-glycan interactions. However, no matter animal protein or plant protein has been reported, it is the interaction between hydrophilic protein and hydrophilic polysaccharide. So far, the systematic study and industrial application potential of the interaction of hydrophobic proteins and hydrophilic polysaccharides have been rarely explored. [0004] Alcohol-soluble hydrophobic proteins (take gliadin as an example) can form nano-scale granular aggregate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/12A23L33/125A23L33/185
CPCA23L33/12A23L33/125A23L33/185A23V2002/00A23V2250/5486A23V2250/511
Inventor 袁杨李国燕李梦帆
Owner GUANGZHOU UNIVERSITY
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