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A molecular imprinting electrochemical detection method for ketamine drug

A technology of molecular imprinting and detection method, which is applied in the field of electrochemical detection of molecular imprinting of ketamine drugs, can solve the problems of long detection period, influence on detection results, and inability to complete on-site rapid detection, and achieve short detection time, high detection sensitivity, and high detection efficiency. short cycle effect

Active Publication Date: 2021-09-24
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a molecularly imprinted electrochemical detection method for ketamine drugs, which can be directly used for the detection of human urine without the need for sample pretreatment, so as to solve the problem of interference in the detection of biological samples in existing detection methods As a result, a large amount of pretreatment work is required for the samples, the detection period is long, and the on-site rapid detection cannot be completed

Method used

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  • A molecular imprinting electrochemical detection method for ketamine drug
  • A molecular imprinting electrochemical detection method for ketamine drug
  • A molecular imprinting electrochemical detection method for ketamine drug

Examples

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Embodiment 1

[0029] A molecular imprinting electrochemical detection method for ketamine drugs proposed by the present invention comprises the following steps:

[0030] 1. Preparation of high-purity graphene-loaded MOFs suspension: 1 mg / ml graphene and 1 mg / ml MOFs suspension were ultrasonically treated by an ultrasonic processor for 3-4 hours, so that MOFs were evenly loaded on the graphene sheet.

[0031] 2. Preparation of graphene / MOFs modified electrode: Take 3 μL of high-purity graphene-loaded MOFs suspension, drop-coat it on the surface of the screen-printed electrode twice, and let it dry naturally to obtain a graphene / MOFs modified electrode.

[0032] 3. Take 2 μL of molecularly imprinted polymer solution and drop-coat it on the graphene / MOFs modified electrode, place the UV lamp 22cm directly above the screen-printed electrode in a dark box, and UV-induced polymerization for 180 minutes to obtain the MIM / RGO / MOFs modification electrode.

[0033] Scanning electron microscopy exper...

Embodiment 2

[0041] In order to further optimize the technical scheme in Example 1, the amount of high-purity graphene-supported MOFs suspension in this example is 1.5-4 μL. When the amount of high-purity graphene suspension is less than 3 μL, the peak current increases with the increase of the dosage, and after more than 3 μL, the peak current decreases with the increase of the dosage. It is recommended to choose 3 μL as the optimal dosage.

Embodiment 3

[0043] In order to further optimize the technical scheme in Example 1 and the polymerization conditions of the molecularly imprinted polymeric membrane, in this example, ketamine is selected as the template molecule, MAA is used as the functional monomer and the crosslinking agent EGDMA, and the molar ratio of the three is 1:4 : 60; the removal of template molecules in the polymembrane selects methyl alcohol: acetic acid is 9: 1 (volume ratio) as eluent with the speed elution of 180 revolutions per second 30min, after washing several times with deionized water; The adsorption time is selected as 10min. At this point, the adsorption of ketamine on the blot reached equilibrium. Because the incubation time continues to increase, the peak current basically does not change.

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Abstract

The invention relates to the technical field of drug detection, in particular to a molecular imprinted electrochemical detection method for ketamine drugs. The detection method uses a modified screen-printed electrode as a working electrode, and utilizes cyclic voltammetry or differential pulse voltammetry to test the Ketamine-like drugs were measured, and the screen-printed electrodes were modified by graphene RGO and MOFs composite nanomaterials and molecularly imprinted polymer membranes, respectively, and then washed, and ketamine was added to the buffer solution. The method of the present invention has high sensitivity and good selectivity, and can be directly used for the detection of human urine without sample pretreatment, so as to solve the problem of the influence of interference substances in biological test materials on the detection results in the existing detection methods, a large number of samples need to be carried out The preprocessing work, the detection cycle is long, and the on-site rapid detection cannot be completed.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a molecular imprint electrochemical detection method for ketamine drugs, which is used for detecting whether ketamine is contained in human urine. Background technique [0002] Ketamine, the main component of "K powder", chemical name: 2-(2-chlorophenyl)-2-(methylamino)cyclohexanone, molecular formula: C 13 h 16 CINO, a phencyclidine hydrochloride derivative, is a dissociative anesthetic and a noncompetitive antagonist of the N-methyl-D-aspartate (NMDA) subtype of excitatory amino acid receptors. Ketamine can play an antidepressant role by antagonizing NMDA receptors and regulating AMPA receptor throughput. Sedative drugs originally approved by the Food and Drug Administration for the induction and maintenance of anesthesia in adults have rapid and sustained antidepressant effects in depressed patients, including those with treatment-resistant depression. Clinically use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 白慧萍付开新王世雄张瑞林张艮林柳清菊
Owner YUNNAN UNIV
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