A preparation method of hydrogel composite biological patch for repairing abdominal wall defect
A biological patch and hydrogel technology, applied in the field of biomedical tissue engineering, can solve the problem of difficult vascularization and oxygen, nutrient exchange and metabolite discharge, difficult to grow host cells and new tissues, and poor mechanical properties. It can help the growth of cells and tissues, promote the neogenesis and vascularization of abdominal wall tissue, and control the mechanical strength and degradation rate.
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Embodiment example 1
[0022] see Figure 1 to Figure 3 , the preparation method of the hydrogel composite biological patch for repairing abdominal wall defects, the specific preparation steps are as follows:
[0023] a. Design of the mold structure: the mold consists of two parts: the mold base and the jacket. There are evenly distributed vertical cylinders on the base. The distance between adjacent cylinders is 500-800 microns, and the diameter of the cylinders is 500±5 microns. The shape of the mold is and size can be designed according to demand;
[0024] b. Preparation of pouring solution: Weigh a set amount of gelatin in a beaker, add deionized water, stir and dissolve in water at 60 degrees Celsius, and make a gelatin solution with a concentration of 15±1%; then weigh the biological crosslinking agent microorganism The mass ratio of transglutaminase to gelatin is 1:10±0.1; wait for the temperature of the gelatin solution to drop to room temperature, add the cross-linking agent and stir rapi...
Embodiment example 2
[0027] In this example, refer to figure 1 , figure 2 , image 3 , the preparation method of the hydrogel composite biological patch for repairing abdominal wall defects, using porcine dermis decellularized matrix powder and gelatin, through the method of mold casting and enzymatic cross-linking to prepare a hydrogel containing pre-vascularized channels Glue composite biological patch scaffold, the specific preparation steps are as follows:
[0028]a. Preparation of pouring solution: place the beaker on the electronic scale, first adjust to zero and then weigh the set amount of gelatin in the beaker, then add deionized water to the beaker to make the gelatin concentration 15%, and then put Its beaker is placed in the water bath of 60 ℃ and fully stirred, and its gelatin is all dissolved; Then take the biological cross-linking agent microbial transglutaminase of the gelatin of setting amount again, the weight ratio of its amount and gelatin is 1: 10. Put the gelatin solution...
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