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Pearl powder artificial bone supporting material with multi-stage micro-nano structure and technique for producing the same

A scaffold material and pearl powder technology, applied in medical science, prosthesis, etc., can solve the problems of poor material plasticity and formability, poor porosity and connectivity, and loss of activity of active ingredients, and achieve improved biocompatibility and good Biocompatibility, regeneration-promoting effect

Active Publication Date: 2009-08-05
REGENOVO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pearl powder is used as an artificial bone material, but the key problem to be solved is that the material has poor plasticity and formability, and it is difficult to form a structure with good porosity and connectivity
However, the commonly used methods such as chemical foaming and high-temperature sintering have poor molding effects on pearl powder, and can easily lead to the inactivation of the active ingredients in pearl powder that have osteoinductive effects. Therefore, a suitable and advanced porous scaffold manufacturing process is selected, including auxiliary materials and The choice of molding method is the key issue of whether the pearl powder material can be effectively used in bone repair and regeneration

Method used

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  • Pearl powder artificial bone supporting material with multi-stage micro-nano structure and technique for producing the same
  • Pearl powder artificial bone supporting material with multi-stage micro-nano structure and technique for producing the same
  • Pearl powder artificial bone supporting material with multi-stage micro-nano structure and technique for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take pearl powder (Zhejiang Shanxia Lake Pearl Group), and use a vibrating sieve to sieve it with -2000 mesh and +3000 mesh to obtain pearl powder with a diameter of 1 μm-10 μm, and then degrease the pearl powder with 1:1 chloroform / methanol for 12 hours ; Soak in PBS+NaOH+NaN3 mixed solution for 12 hours at 37°C and rinse with triple distilled water; 200g / L H at 37°C 2 o 2 Soak for 6 hours to partially deproteinize; rinse with triple-distilled water for 6 times, put in a constant temperature drying oven at 37°C to dry; seal the package, and sterilize with cobalt 60 for later use.

[0038] PLGA / PLA (3:1) was dissolved in 1,4-dioxane (Shanghai Chemical Reagent Purchase and Supply Wulian Chemical Factory, analytically pure) organic solvent according to the concentration of 15% (w / v), stirred and dissolved at 40°C, The cloud point of the formed liquid-liquid phase separation system is 28°C; add pearl powder according to the ratio of PLGA / PLA: pearl powder=70 / 30 (w / w) to p...

Embodiment 2

[0042] Take pearl powder (Zhejiang Shanxia Lake Pearl Group), and sieve it with a vibrating sieve with 2000 meshes to obtain pearl powder with a diameter of 3-6 μm, and then degrease the pearl powder with 1:1 chloroform / methanol for 12 hours; 37°C PBS+NaOH +NaN3 mixed solution soaked for 12h and then rinsed with triple distilled water; 37°C 200g / L H 2 o 2 Soak for 24 hours to completely deproteinize; rinse with triple-distilled water for 6 times, put in a constant temperature drying oven at 37°C to dry; seal the package, and sterilize with Co60 irradiation for later use.

[0043] Configuration 1, the mixed solution of 4-dioxane (Shanghai Chemical Reagent Purchase and Supply Wulian Chemical Factory, analytically pure) and water, wherein the content of water in the mixed solution is 5% (V 水 / V 总 ). PLGA / PLA (4:1) was dissolved in the mixed liquid at a concentration of 15% (w / v), stirred and dissolved at 40°C; pearls were added according to the ratio of (PLGA+PLA) / pearl powder...

Embodiment 3

[0047] Get the composite material scaffold prepared in Example 1 and New Zealand white rabbit bone marrow mesenchymal stem cells (MSCs), and co-culture them in a three-dimensional cell / tissue culture system (RCCS TM , NASA), cultured for 10 days, fixed with glutaraldehyde at 4°C, fixed with acetic acid at 4°C for 1h, double stained with uranyl acetate-lead citrate, and observed the structure with a scanning electron microscope (Figure 3). It shows that the cells grow, proliferate, and migrate well in the three-dimensional scaffold structure. The cells stretch in a certain direction in the three-dimensional space, and form a network structure in the pores, leaving channels for nutrients to pass through.

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Abstract

The invention relates to a novel biological scaffold material for bone repair and a preparation method thereof. PLGA and PLA with a mass ratio of 1: 1-10: 1 are dissolved into chloroform, dimethyl sulfoxide, 1, 4-dioxane or a mixed liquid of the 1, 4-dioxane and ultrapure water, and then pearl powder which is subjected to partial or complete deproteinization treatment is added into the obtained product according to the proportion of the PLGA / a PLA mixture to the pearl powder (mass ratio) is 1: 1-10: 1 to obtain forming slurry. A three-dimensional scaffold with high porosity and connectivity rate is designed through a 3D software, and then a low-temperature rapid forming system is utilized to ensure that the three-dimensional scaffold is formed to obtain a scaffold with a microporous structure. An artificial bone biological scaffold material prepared by the method has a three-dimensional scaffold structure that a macroscopic structure has aperture channels with diameters of between 100 and 500mu m and a microstructure has micropores with diameters of between 10 and 20mu m, wherein micron pearl powder is dispersedly distributed on walls of the micropores. The porosity is between 60 and 90 percent, and a macroporous structure is perforated in three directions of X axis, Y axis and Z axis, and has 100 percent of connectivity.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a new bio-scaffold material for bone repair and a preparation method thereof. Background of the invention [0002] At present, there is an urgent and huge market demand for artificial bone tissue with good physiological functions and no rejection for bone defect repair in the world. In the field of artificial bone manufacturing, foreign research institutions and enterprises have invested a lot of resources in recent years and achieved a series of research results. Some artificial bone materials for defect repair have begun to enter clinical applications. At the same time, there are still many problems to be solved in the core issues of bone scaffold materials such as biocompatibility, osteoinductivity, porosity, and mechanical strength. [0003] Pearl powder is an ideal natural bone-forming material, which has the characteristics of biocompatibility and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/56A61L27/02
Inventor 徐铭恩索海瑞王秋君李艳蕾
Owner REGENOVO BIOTECH
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