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Black bean identification method based on thin-layer chromatography

A technology of thin-layer chromatography and black beans, which is applied in the field of detection of pharmaceutical raw materials, can solve the problems of irregular spots, difficulty in meeting the requirements of identification and identification efficiency, and no spots in inspection, so as to improve the first-time success rate, reduce cover-up, and ensure spots rounded effect

Active Publication Date: 2019-06-21
GUANGZHOU BAIYUNSHAN ZHONGYI PHARMA COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Thin-layer chromatography is the main method to evaluate the quality of black soybeans. However, in traditional black soybean TLC analysis, there are often phenomena such as no spots and irregular spots. For this reason, multiple samples and multiple expansions are required to obtain identification results. , so it is difficult to meet the requirements of identification efficiency in actual production

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  • Black bean identification method based on thin-layer chromatography

Examples

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Effect test

Embodiment 1

[0028] The embodiment of the present invention provides a method for identifying black beans based on thin-layer chromatography, comprising the steps of:

[0029] Preparation of the test solution: take 2 g of this product powder, add 20 ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 1 ml of methanol to dissolve the residue, and use it as the test solution;

[0030] Preparation of medicinal material reference solution: take another 2 g of black soybean reference medicinal material, and prepare a reference medicinal material solution in the same way;

[0031] Preparation of pure reference solution: take daidzin reference substance and daidzein reference substance, add methanol to make solutions containing 1mg per 1ml respectively, as the reference substance solution;

[0032] Spotting: According to the TLC (Appendix VIB) test, draw 5 μl of each of the above four solutions, and spot them on the same silica gel G thin-layer plate;

[0033]...

Embodiment 2

[0037] The embodiment of the present invention provides a method for identifying black beans based on thin-layer chromatography, comprising the steps of:

[0038] Preparation of the test solution: take 2 g of this product powder, add 20 ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 1 ml of methanol to dissolve the residue, and use it as the test solution;

[0039] Preparation of medicinal material reference solution: take another 2 g of black soybean reference medicinal material, and prepare a reference medicinal material solution in the same way;

[0040] Preparation of pure reference solution: take daidzin reference substance and daidzein reference substance, add methanol to make solutions containing 1mg per 1ml respectively, as the reference substance solution;

[0041] Spotting: According to the TLC (Appendix VIB) test, draw 5 μl of each of the above four solutions, and spot them on the same silica gel G thin-layer plate;

[0042]...

Embodiment 3

[0046] The embodiment of the present invention provides a method for identifying black beans based on thin-layer chromatography, comprising the steps of:

[0047] Preparation of the test solution: take 2 g of this product powder, add 20 ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 1 ml of methanol to dissolve the residue, and use it as the test solution;

[0048] Preparation of medicinal material reference solution: take another 2 g of black soybean reference medicinal material, and prepare a reference medicinal material solution in the same way;

[0049]Preparation of pure reference solution: take daidzin reference substance and daidzein reference substance, add methanol to make solutions containing 1mg per 1ml respectively, as the reference substance solution;

[0050] Spotting: according to the TLC (Chinese Pharmacopoeia Appendix VIB) test, draw 5 μl of each of the above four solutions, and spot them on the same silica gel G thin-l...

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Abstract

The invention provides a black bean identification method based on thin-layer chromatography. The black bean identification method comprises the following steps: preparing a test solution; preparing amedicinal material control solution; performing thin-layer chromatography; respectively sucking the test solution and the medicinal material control solution and performing sample application on thesame silica gel G thin-layer plate; placing the silica gel G thin-layer plate subjected to sample application in steam of a developing agent for pre-saturation, then placing the silica gel G thin-layer plate in the developing agent for developing, taking out the silica gel G thin-layer plate after developing, airing, heating the aired silica gel G thin-layer plate at the temperature of not lower than 100 DEG C, and then inspecting, so as to obtain the silica gel G thin-layer plate, wherein the developing agent is a mixture of toluene, methanol and formic acid. Compared with the prior art, thedevelopment frequency of black bean thin layer identification is reduced to one time from average 3.3 times traditionally, namely, clear chromatography can be obtained through one-time sample application, one-time development and one-time inspection, the one-time success rate of black bean thin layer identification is remarkably improved, and the requirement on black bean identification efficiencyin actual production is met.

Description

technical field [0001] The invention relates to the field of detection of pharmaceutical raw materials, in particular to a black soybean identification method based on thin-layer chromatography. Background technique [0002] Black beans are one of the main ingredients of Guxian tablets. Its nature and flavor are sweet, flat, and return to the spleen and kidney channels. Beneficial for eyesight, nourishing blood and expelling wind, benefiting water and detoxification. It can be used to treat yin deficiency and polydipsia, dizziness, dizziness, hyperhidrosis due to physical deficiency, low back pain due to kidney deficiency, edema and oliguria, arthralgia and spasm, numbness in hands and feet, and food poisoning. [0003] Thin-layer chromatography (TLC) is a trace, rapid and simple separation and analysis method. Apply the appropriate stationary phase to a glass plate, plastic or aluminum substrate to form a uniform thin layer. After spotting and unfolding, compare the rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/94
Inventor 邝永全叶佩姗卢金玲姚敏菲胡家敏谢晓玲黎炳华刘芮菁
Owner GUANGZHOU BAIYUNSHAN ZHONGYI PHARMA COMPANY
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