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Method for quantitatively and traceably measuring mussel mucoprotein degradation

A mussel mucin, traceable technology, applied in the field of biodegradation of medical biomacromolecules, can solve problems such as insufficient measurement accuracy, inability to obtain degradation data, and inability to measure in situ in real time, achieving rapid evaluation and avoiding the effect of experimental operations

Inactive Publication Date: 2019-05-28
JIANGYIN USUN BIOCHEMICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the above method or measurement accuracy is not enough to obtain the degradation status of the device under real use conditions; or cannot obtain real degradation data due to in-situ measurement in real time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Radioactive iodine-labeled mussel mucin polymer particles are used to study the degradation of mussel mucin in cell culture medium

[0026] Preparation of radioactive iodine-labeled mussel mucin: Iodogen was used as an oxidant to iodine-label mussel mucin, and 125I was directly introduced into the tyrosine residue in the molecule. Before labeling, dissolve lodogen in an organic solvent, apply to the bottom of the reaction tube, and allow to dry. A 1.0 mg / ml mussel mucin solution was placed in a reaction tube and placed in an ice bath. During iodination, the weight ratio of 125I to protein molecules is 1-1.2. Stir gently and continuously for 10 min, transfer the reaction mixture from the reaction tube, and stop the reaction.

[0027] Preparation of mussel mucin polymer particles: Take 10ml of iodine-labeled mussel mucin solution with a concentration of 1.0 mg / ml, and keep stirring for 24 hours under the condition of pH 7.0, so that mussel mucin is cross-link...

Embodiment 2

[0031] Example 2: Radioactive iodine-labeled mussel mucin coated artificial bone particles for studying the degradation of mussel mucin in tissue homogenate

[0032] Preparation of radioactive iodine-labeled mussel mucin: Iodogen was used as an oxidant to iodine-label mussel mucin, and 125I was directly introduced into the tyrosine residue in the molecule. Before labeling, dissolve lodogen in an organic solvent, apply to the bottom of the reaction tube, and allow to dry. A 1.0 mg / ml mussel mucin solution was placed in a reaction tube and placed in an ice bath. During iodination, the weight ratio of 125I to protein molecules is 1-1.2. Stir gently and continuously for 10 min, transfer the reaction mixture from the reaction tube, and stop the reaction.

[0033] Preparation of mussel mucin-coated artificial bone particles: take 5 g of artificial bone particles with a diameter of 0.25-1 mm (Shanghai Hayan Biotechnology Co., Ltd.) and 5 ml of iodine-labeled mussel mucin solution w...

Embodiment 3

[0037] Example 3: Artificial bone particles coated with radioiodine-labeled mussel mucin were used to study the degradation of mussel mucin in mice

[0038] Preparation of radioactive iodine-labeled mussel mucin: Iodogen was used as an oxidant to iodine-label mussel mucin, and 125I was directly introduced into the tyrosine residue in the molecule. Before labeling, dissolve lodogen in an organic solvent, apply to the bottom of the reaction tube, and allow to dry. A 1.0 mg / ml mussel mucin solution was placed in a reaction tube and placed in an ice bath. During iodination, the weight ratio of 125I to protein molecules is 1-1.2. Stir gently and continuously for 10 min, transfer the reaction mixture from the reaction tube, and stop the reaction.

[0039] Preparation of mussel mucin-coated artificial bone particles: take 5 g of artificial bone particles with a diameter of 0.25-1 mm (Shanghai Hayan Biotechnology Co., Ltd.) and 5 ml of iodine-labeled mussel mucin solution with a con...

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Abstract

The invention belongs to the technical field of biology, and relates to a method for quantitatively and traceably studying mussel mucoprotein degradation. The method comprises the steps that 1, aftermussel mucoprotein is combined with radioactive elements or a fluorescent reagent, a polymer is formed through intermolecular cross-linking, or the mussel mucoprotein covers a biomedical material to be cured, and mussel mucoprotein microballoon spheres are formed; 2, an in-vitro model and an in-vivo model are built; 3, by means of the in-vivo or / and in-vitro model, increase of a marker in supernate and reduction of the marker on the mussel mucoprotein polymer / covered microballoon spheres are qualitatively and quantitatively measured, and therefore the degradation characteristic and degradation rate of the mussel mucoprotein in different environment are determined. According to the method, the defect of the biomedical macromolecule degradation research methodology is overcome, and the study method is dynamic and quantitative.

Description

technical field [0001] The invention relates to the field of medical biomacromolecule degradation, and more particularly, relates to a method for measuring mussel mucin degradation polymers and using the polymers to determine mussel mucin degradation. Background technique [0002] Mussel adhesive protein (MAP), also known as Mytilusedulis foot protein (Mefp), is the marine shellfish Mytilus edulis Linnaeus, Mytilus coruscus, A special protein secreted by the emerald mussel (Perna viridis). Mussels are usually attached to the reefs along the coast or the bottom of ships in groups, and have the ability to withstand wave impact in the offshore. In fact, mussels can be extremely firmly attached to almost any material substrate, such as metal, wood, glass, etc. The main reason why mussels have the above characteristics is that their silk glands can produce and store this special mucin, and mussels release mucin to solid surfaces such as rocks through silk to form a water-resist...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/60G01N33/58
Inventor 拉斯·赫尔曼顾铭母瑞红
Owner JIANGYIN USUN BIOCHEMICAL TECH CO LTD
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