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An iconic microRNA marker and its application for detecting the passage effect of spermatogenesis disorder

A technology of sperm production and markers, applied in the fields of biotechnology and reproductive medicine, can solve the problems of long observation time, achieve the effects of easy detection, avoid long-term experiments, and improve sensitivity and specificity

Active Publication Date: 2019-04-16
NANJING HANWEI PUBLIC HEALTH RES INST CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If we want to observe the passage effect, we need to observe it for a long time. How to judge the sperm production disorder caused by the environmental change in the early stage is a passage effect, which has not been studied yet.

Method used

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  • An iconic microRNA marker and its application for detecting the passage effect of spermatogenesis disorder
  • An iconic microRNA marker and its application for detecting the passage effect of spermatogenesis disorder
  • An iconic microRNA marker and its application for detecting the passage effect of spermatogenesis disorder

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Embodiment Construction

[0026] The present invention is described in detail as follows:

[0027] The present inventor gave DBP treatment with the standard operating procedure (SOP) in the E8-14 days of rat pregnancy, collected the testicular tissues of the F1-F3 generation, and adopted RT-PCR method, TaqMan miRNA Array, Real-time PCR (TaqMan Probe and dye method) one or more methods for detection.

[0028] The experimental method of the research mainly includes the following parts:

[0029] 1. Rat generation effect model establishment and basic data collection

[0030] 1. Rats were used as animal models, exposed to DBP by intragastric administration on the 8th to 14th day of pregnancy, and corn oil was given as a control to obtain F1 generations, and F2 and F3 generations were obtained after mutual mating of different litters. Offspring were no longer exposed to DBP except during pregnancy.

[0031] 2. Male rats were 90-180 days of adulthood, after euthanasia, the testis tissue was taken, and the ...

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Abstract

The invention relates to a symbolic micro RNA marker for detecting a spermatogenic failure passage effect and application thereof, belongs to the field of biotechnology and reproductive medicine, and discloses a micro RNA (miRNA) marker for detecting a spermatogenic failure passage effect and application thereof. The marker is a plurality selected from a group consisting of rho-miR-1-3p, rho-miR-124-3p and rho-miR-190a-5p. The marker has specificity and sensitivity for monitoring the spermatogenic failure passage effect, provides a reliable detection basis and quick detection method for diagnosis monitoring of the spermatogenic failure passage effect and has important application values.

Description

field of invention [0001] The invention belongs to the fields of biotechnology and reproductive medicine, and relates to a microRNA (miRNA) marker used for detecting the passage effect of spermatogenesis disorder and an application thereof. Background technique [0002] Currently, about 10-15% of couples of reproductive age worldwide suffer from fertility disorders. Due to the large population base in my country, the number of infertility patients among newly married couples is far more than one million, and the infertility caused by male factors is higher than 50%. Impairment of spermatogenesis has become the most common disease of male infertility. Sperm production is a complex process, and many factors including environmental factors regulate and affect the development and maturation of sperm, which can lead to spermatogenesis disorders. For half a century, men's sperm counts have declined considerably. This suggests that the increase in the incidence of spermatogenic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 吴炜袁蓓蕾汤秋勤芮璨居蓉陈丽平夏彦恺王心如
Owner NANJING HANWEI PUBLIC HEALTH RES INST CO LTD
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